A CRC–MYB-mediated local chromatin loop at the MAB4 locus determines auxin-mediated nectary size in Arabidopsis thaliana

A CRC–MYB-mediated local chromatin loop at the MAB4 locus determines auxin-mediated nectary size in Arabidopsis thaliana | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article A CRC–MYB-mediated local chromatin loop at the MAB4 locus determines auxin-mediated nectary size in Arabidopsis thaliana Hideaki Iimura, Makoto Takahashi, Ryosuke Ohta, Asumi Nagamori, and 11 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7421252/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract The nectaries of flowers in angiosperms are typically located at the base of the stamens and secrete nectar, composed primarily of sugars and various secondary metabolites, either actively or passively. Although nectaries develop in specific locations and perform specialized functions, the regulatory mechanisms governing their formation are unclear. Through a pseudotime trajectory analysis based on single-nucleus RNA-seq data and newly performed spatial transcriptomics, we uncover a spatiotemporal regulatory network governing nectary development. We establish CRABS CLAW (CRC) as a master regulator that integrates temporal transcriptional regulation with spatial auxin transport, orchestrating nectary development through phytohormone signaling, sugar transport and terpene biosynthesis pathways. CRC interacts to MYB21, MYB24 and MYB57, with the CRC–MYB57 complex binding the 5′ and 3′ regulatory regions of the MACCHI-BOU 4 ( MAB4 ) locus, facilitating the formation of a chromatin loop that enhances MAB4 expression. This loop is necessary for the development of properly sized nectaries. MAB4 reinforces PIN-FORMED 1 (PIN1)-mediated auxin transport to establish auxin maxima at the nectary tip and regulates PIN6 to maintain auxin gradients. These findings reveal how CRC integrates transcriptional regulation and auxin transport to coordinate nectary development, linking metabolic output with organogenesis. Biological sciences/Plant sciences/Plant development/Plant morphogenesis Biological sciences/Plant sciences/Plant reproduction/Fruiting Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupplementaryData2.xlsx Supplementary Data 2 SupplementaryData5.xlsx Supplementary Data 5 SupplementaryData4.xlsx Supplementary Data 4 SupplementaryData10.xlsx Supplementary Data 10 SupplementaryData6.xlsx Supplementary Data 6 SupplementaryData9.xlsx Supplementary Data 9 SupplementaryData7.xlsx Supplementary Data 7 SupplementaryData8.xlsx Supplementary Data 8 SupplementaryData3.xlsx Supplementary Data 3 3.SupplementaryFig0821825iimura.pdf Supplemental Figures SupplementaryData1.xlsx Supplementary Data 1 Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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thaliana","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-7421252/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7421252/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"The nectaries of flowers in angiosperms are typically located at the base of the stamens and secrete nectar, composed primarily of sugars and various secondary metabolites, either actively or passively. Although nectaries develop in specific locations and perform specialized functions, the regulatory mechanisms governing their formation are unclear. Through a pseudotime trajectory analysis based on single-nucleus RNA-seq data and newly performed spatial transcriptomics, we uncover a spatiotemporal regulatory network governing nectary development. We establish CRABS CLAW (CRC) as a master regulator that integrates temporal transcriptional regulation with spatial auxin transport, orchestrating nectary development through phytohormone signaling, sugar transport and terpene biosynthesis pathways. CRC interacts to MYB21, MYB24 and MYB57, with the CRC–MYB57 complex binding the 5′ and 3′ regulatory regions of the \u003ci\u003eMACCHI-BOU 4\u003c/i\u003e (\u003ci\u003eMAB4\u003c/i\u003e) locus, facilitating the formation of a chromatin loop that enhances \u003ci\u003eMAB4\u003c/i\u003e expression. This loop is necessary for the development of properly sized nectaries. MAB4 reinforces PIN-FORMED 1 (PIN1)-mediated auxin transport to establish auxin maxima at the nectary tip and regulates PIN6 to maintain auxin gradients. 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