Boosting effects of MiR-4262 on acute myeloid leukemia advancement via governing KLF6

Abstract Background: Purpose of this study was to explore the influence of miR-4262 on the progression of acute myeloid leukemia (AML) and its molecular mechanism.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to assess the expression of miR-4262 in AML serum and cell lines. MTT, Transwell assays and flow cytometry were adopted to investigate the effect of miR-4262 on cell proliferation, invasion, migration and apoptosis abilities of HL-60 cells respectively. Luciferase reporter assay was conducted to reveal the target relationship of miR-4262 and KLF6. Western blot analysis was utilized to evaluate the expression level of proteins.Results: Relative expression of miR-4262 was up-regulated in AML serum and cell lines (P<0.05). miR-4262 expression was closely related to FAB classification (P=0.002) of AML patients. miR-4262 mimics could promotes the proliferation, invasion and migration of HL-60 cells, while miR-4262 inhibitor is obviously weakened these biological behaviors. Luciferase assay illustrated that miR-4262 can directly interact with KLF6 3’UTR. Up-regulation of miR-4262 could decrease KLF6 level, and increase EGFR level, while the down-regulation of miR-4262 showed the opposite results. Moreover, KLF6 gene knockdown reversed the results caused by miR-4262 inhibitor (P<0.05). Inhibition of KLF6 could significantly promoted the proliferation, invasion and migration of HL-60 cells which is caused by miR-4262 inhibitor.Conclusions: miR-4262 was obviously increased in AML serum and cells, it promotes the progression of AML by regulating KLF6.