Qualitative and Quantitative Tests on the Ingredients of Mahkota Dewa (Phaleria macrocarpa) Fruit Extract as an Alternative Therapy for Infertility in Endometriosis

Endometriosis is a gynecological disease with complaints of pain, infertility, or both, up to 35-50%. The fruit extract of Mahkota Dewa (Phaleria macrocarpa) contains antioxidants and anti-inflammatories, has anticancer prospects and is expected to have ingredients as an alternative therapy for treating infertility in endometriosis. To conduct qualitative and quantitative tests on the content of Phaleria macrocarpa fruit extract, which is expected to contain substances that act as an alternative to infertility therapy in endometriosis. Mahkota Dewa (Phaleria macrocarpa) was ripened, and its seeds were extracted using 95% ethanol twice. The results of extracts 1 and 2 were combined and then examined by Thin Layer Chromatography (TLC). The research was conducted at the Pharmacognosy-Phytochemical Laboratory, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Qualitative test showed that 95% ethanol extract of Phaleria macrocarpa fruit contains flavonoids, alkaloids, terpenoids, and steroids. Quantitative test using Thin Layer Chromatography (TLC) method obtained a flavonoid content of 131.51-189.89µg/ml, equivalent to quercetin, in 6 repetitions, with a wavelength of 510 nm. Phaleria macrocarpa fruit contained flavonoids, alkaloids, terpenoids and steroids. The level of steroids contained in the fruit extract of Mahkota Dewa (Phaleria macrocarpa) had not been obtained in this study. Cite this article: Ivanna Beru Brahmana, Purwanto, Soetrisno, Bambang Purwanto, Eti Poncorini Pamungkasari. Qualitative and Quantitative Tests on the Ingredients of Mahkota Dewa (Phaleria macrocarpa) Fruit Extract as an Alternative Therapy for Infertility in Endometriosis. Research Journal of Pharmacy and Technology. 2024; 17(8):3814-0. doi: 10.52711/0974-360X.2024.00592 Cite(Electronic): Ivanna Beru Brahmana, Purwanto, Soetrisno, Bambang Purwanto, Eti Poncorini Pamungkasari. Qualitative and Quantitative Tests on the Ingredients of Mahkota Dewa (Phaleria macrocarpa) Fruit Extract as an Alternative Therapy for Infertility in Endometriosis. Research Journal of Pharmacy and Technology. 2024; 17(8):3814-0. doi: 10.52711/0974-360X.2024.00592 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-8-40

1. Meuleman C, Vandenabeele B, Fieuws S, Spiessens C, Timmerman D, D’Hooghe T. High Prevalence of Endometriosis in Infertile Women with Normal Ovulation and Normospermic Partners. Am Soc Reproduvtive Med [Internet]. 2009; 92(1): 68–74. doi.org/10.1016/j.fertnstert.2008.04.056 2. Burney RO, Giudice LC. Pathogenesis and pathophysiology of endometriosis. Fertil Steril [Internet]. 2012; 98(3): 511–9. http://dx.doi.org/10.1016/j.fertnstert.2012.06.029 3. Kitawaki J, Kado N, Ishihara H, Koshiba H, Kitaoka Y, Honjo H. Endometriosis: The Pathophysiology as an Estrogen-dependent Disease. J Steroid Biochem & Mollecular Biol [Internet]. 2003; 83: 149–55. www.elsevier.com/locate/jsbmb 4. Faried A, Bolly H, Septiani L, Kurnia D, Arifin M, Wirakusumah F. Potential of Indonesian Herbal Medicine, Phaleria macrocarpa (Scheff.) Boerl, for Targeting Multiple Malignancy Signaling Pathways: An Introductory Overview. European J Med Plants [Internet]. 2016; 11(2): 1–17. doi.org/10.9734/ejmp/2016/20760 5. Azmir J, Zaidul ISM, Sharif KM, Uddin MS, Jahurul MHA, Jinap S, et al. Supercritical carbon dioxide extraction of highly unsaturated oil from Phaleria macrocarpa seed. Food Res Int [Internet]. 2014; 65(PC): 394–400. http://dx.doi.org/10.1016/j.foodres.2014.06.049 6. Alara O, Olalere O. A Critical Overview on the Extraction of Bioactive Compounds from Phaleria macrocarpa (Thymelaceae). Nat Prod Chem Res [Internet]. 2016; 4(5): 1–4. doi.org/10.4172/2329-6836.1000232 7. Triastuti A, Park HJ, Choi J. Phaleria macrocarpa suppresses oxidative stress in alloxan-induced diabetic rats by enhancing hepatic antioxidant enzyme activity. Nat Prod Sci [Internet]. 2009; 15(1): 37–43. doi.org/G704-001403.2009.15.1.002 8. Fariza IN, Fadzureena J, Zunoliza A, Chuah AL, Pin K., Adawiah I. Anti-inflammatory Activity of the Major Compound from Methanol Extract of Phaleria macrocarpa Leaves. J Appl Sci [Internet]. 2012; 12(11): 1195–8. doi.org/10.3923/jas.2012.1195.1198 9. Hendra P. Review: peluang mahkota dewa sebagai antikanker. J Farm Sains dan Komunitas [Internet]. 2012; 9(2): 104–7. https://doi.org/10.24071/jpsc.0078 10. Hanif M, Yuandani, Harahap U. Evaluation of Toxic Effect of Phaleria macrocarpa (Scheff.) Boerl Leaf Extract on Hematological Parameters. Asian J Pharm Res Dev [Internet]. 2020; 8(3): 01–4. http://ajprd.com 11. Hanifah RS, Novitarani NA, Harmen F, Tedjo A, Azizah NN, Putrianingsih R, et al. The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line. Res J Pharm Technol [Internet]. 2019; 12(6): 2902–6. doi.org/10.5958/0974-360x.2019.00489.x 12. Annadurai P. Extraction and Isolation of Bioactive Compounds from Lantana camara Leaves by Column Chromatographic Techniques. Res J Pharm Technol [Internet]. 2021; 14(3): 1607–11. doi.org/10.5958/0974-360X.2021.00285.7 13. Devgun M, Nanda A, Ansari SH, Swamy SK. Recent Techniques for Extraction of Natural Products. Res J Pharm Tech [Internet]. 2010; 3(3): 644–9. www.rjptonline.org 14. Thomas J, Gopakumar A, Narendrakumar G, Preethi T V., Chellaiyan V. Extraction, phytochemical screening and antibacterial activity Wattakaka volubilis (Linn F) Stapf. Res J Pharm Technol [Internet]. 2016; 9(4): 355–60. doi.org/10.5958/0974-360X.2016.00063.9 15. Mohamed AA, Ali SI, Sameeh MY, Abd El-Razik TM. Effect of solvents extraction on HPLC profile of phenolic compounds, antioxidant and anticoagulant properties of Origanum vulgare. Res J Pharm Technol [Internet]. 2016; 9(11): 2009–16. doi.org/10.5958/0974-360X.2016.00410.8 16. Chen GL, Chen SG, Xiao Y, Fu NL. Antioxidant capacities and total phenolic contents of 30 flowers. Ind Crops Prod [Internet]. 2018; 111(June 2017): 430–45. https://doi.org/10.1016/j.indcrop.2017.10.051 17. Mehmood A, Ishaq M, Zhao L, Yaqoob S, Safdar B, Nadeem M, et al. Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). Ultrason Sonochem [Internet]. 2019; 51(2019): 12–9. https://doi.org/10.1016/j.ultsonch.2018.10.013 18. Zam W, Ali A, Hasan R. Parameters Influencing Phenolic compounds extraction from Pistacia palaestina fruits during different stages of Maturity. Res J Pharm Technol [Internet]. 2019; 12(12): 6133–7. doi.org/10.5958/0974-360X.2019.01065.5 19. Suganya J, Viswanathan T, Radha M, Rathisre PR, Marimuthu N. Comparative quantitative screening of secondary phytoconstituents from the leaves extract of Sterculia foetida Linn. Res J Pharm Technol [Internet]. 2017; 10(9): 2907–12. doi.org/10.5958/0974-360X.2017.00513.3 20. Gini EJ, Sivakkumar T, Kuppuswami S. Determination of antioxidant activity of various extracts of pajanelia longifolia (Willd.) K. Schum, isolation and characterization of flavonoid from ethanol extract by column chromatography. Res J Pharm Technol [Internet]. 2017; 10(10): 3391–7. doi.org/10.5958/0974-360X.2017.00603.5 21. Maria MS, Sujuti H, Kurnia D, Widjanarko SB. Methanol and ethyl acetate extracts of red fruit (Pandanus conoideus Lam.) short-red showed high of phenolic and radicals scavenging activities. Res J Pharm Technol [Internet]. 2020; 13(9): 4158–64. doi.org/10.5958/0974-360x.2020.00734.9 22. Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutr Sci [Internet]. 2016; 5(e47): 1–15. doi.org/10.1017/jns.2016.41 23. Ullah A, Munir S, Badshah SL, Khan N, Ghani L, Poulson BG, et al. Important Flavonoids and Their Role as a Therapeutic Agent. Molecules [Internet]. 2020; 25(5243): 1–39. doi.org/10.3390/molecules25225243 24. Ozgen S, Kilinc OK, Selamoglu Z. Antioxidant Activity of Quercetin: A Mechanistic Review. Turkish J Agric -Food Sci Technol [Internet]. 2016; 4(12): 1134–8. www.agrifoodscience.com, 25. Ma’ruf MT, Setiawan, Putra BPD. Aktivitas Antibakteri Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) terhadap Staphylococcus aureus. Interdental J Kedokt Gigi [Internet]. 2017;13(2):16–23. https://doi.org/10.46862/interdental.v13i2.360 26. Mak P, Leung YK, Tang WY, Harwood C, Ho SM. Apigenin suppresses cancer cell growth through ERβ. Neoplasia [Internet]. 2006; 8(11): 896–904. doi.org/10.1593/neo.06538 27. Tjandrawinata RR, Nofiarny D, Susanto LW, Hendri P, Clarissa A. Symptomatic treatment of premenstrual syndrome and/or primary dysmenorrhea with DLBS1442, a bioactive extract of Phaleria macrocarpa. Int J Gen Med [Internet]. 2011; 4: 465. doi.org/10.2147/ijgm.s21053 28. Aini HAN, Laksmi DNDI, Setiasih NLE, Purbantoro SD. Pemberian Ekstrak Oncom Hitam dan Merah Memperpanjang Siklus Estrus dan Mempertebal Endometrium Tikus Putih. J Vet [Internet]. 2020; 21(4): 558–64. http://ojs.unud.ac.id/index.php/jvet 29. Widyawaty ED. Pengaruh Meniran Dosis Bertingkat Terhadap Ekspresi IGF-1 Dan Ketebalan Endometrium Pada Mencit Betina Model Endometriosis. J Biosains Pascasarj [Internet]. 2018; 20(1): 9. https://doi.org/10.20473/jbp.v20i1.2018.9-21 30. Lay MM, Karsani SA, Mohajer S, Abd Malek SN. Phytochemical constituents, nutritional values, phenolics, flavonols, flavonoids, antioxidant and cytotoxicity studies on Phaleria macrocarpa (Scheff.) Boerl fruits. BMC Complement Altern Med [Internet]. 2014; 14(152): 1–12. http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=reference&D=emed13&NEWS=N&AN=24885709 31. Balumbi M, Fachruddin F, Risman M. Morfometri Ovarium setelah Pemberian Ekstrak Daun Kelor (Moringa oleifera LAM). Acta Vet Indones [Internet]. 2021; 9(1): 44–52. doi.org/10.29244/avi.9.1.44-52 32. Andjani N, Sujuti H, Winarsih S. Efek Ekstrak Etanol Daun Kelor (Moringa oleifera) terhadap Nuclear Factor Kappa Beta (NF-kB) Aktif dan Apoptosis Cell Line Kanker MCF-7. Maj Kesehat FKUB [Internet]. 2016; 3(4): 204–12. doi.org/10.21776/ub.majalahkesehatan.003.04.6 33. Lay MM, Karsani SA, Mohajer S, Abd Malek SN. Phytochemical constituents, nutritional values, phenolics, flavonols, flavonoids, antioxidant and cytotoxicity studies on Phaleria macrocarpa (Scheff.) Boerl fruits. BMC Complement Altern Med [Internet]. 2014; 14(152): 1–12. doi.org/10.1186/1472-6882-14-152 34. Astriyani W, Surjowardojo P, Susilorini TE. Daya hambat ekstrak buah mahkota dewa (Phaleria macrocarpa L.) dengan pelarut ethanol dan aquades terhadap bakteri staphylococcus aureus penyebab mastitis pada sapi perah. TERNAK Trop J Trop Anim Prod [Internet]. 2017; 18(2): 8–13. https://doi.org/10.21776/ub.jtapro.2017.018.02.2 35. Francis P, Suseem SR. Phytochemical Analysis and Anti-inflammatory Screening of Strychnos colubrina Linn. Res J Pharm Technol [Internet]. 2016; 9(2): 165–9. doi.org/10.5958/0974-360X.2016.00029.9 36. Chak P, Melda S, Chaudhary D, Bisht A, Dwivedi J. Phytochemical and Antioxidant Studies of M. oleifera and A. sativum Flower Extracts. Res J Pharm Technol [Internet]. 2020; 13(10): 4579–84. doi.org/10.5958/0974-360x.2020.00806.9 Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>> RNI: CHHENG00387/33/1/2008-TCDOI: 10.52711/0974-360X 1.3 2021CiteScore 56th percentile Powered by