The Appropriate Acquisition Time Interval Following Injection of 99mTc-Sestamibi with Water Protocol in Single Photon Emission Computed Tomography Myocardial Perfusion Imaging: First Experience in Indonesia

Background: According to EANM/ESC guideline, image acquisitions in stress test should be begun at 30-60 minutes after tracer administration. Our center is a referral hospital for nuclear medicine imaging with many patients but limited number of gamma camera. The shorter time between injection of radiopharmaceutical and imaging acquisition will lead to more examination can be done.The aim of this study was to evaluate the appropriate acquisition time interval with water protocol in 99m Tc-Sestamibi SPECT myocardial perfusion imaging. Methods: Patients who were referred to undergo stress MPI between October 2020 to December 2020 were included in this study. Cardiac stress procedure was performed using treadmill with modified Bruce Protocol. Subjects should drink a total of 330 mL water following 99m Tc-Sestamibi injection. Image acquisitions were performed 10 and 30 minutes afterwards. Quantitative assessment was done by calculated target background ratio (TBR). Statistical analysis was performed using student t-test with Microsoft Excel version 2019. P -value < 0.05 was considered to be statistically significance. Results: Thirty out of 35 subjects were included in this study. Sixteen of them are male and 14 are female with mean of age was 48.7 years old (28 – 80). Mean target background uptake ratio (TBR) in 10- and 30-minutes images were 0.67 (0.44 – 1.11) and 0.76 (0.43 – 1.18) respectively ( p- value = 0.15). Conclusion: There was no significant difference of target to background ratio between acquisition time 10- and 30-minutes following injection of 99m Tc-Sestamibi with water protocol in myocardial perfusion imaging. Trial registration Not applicable.