L1 Gene Molecular Variation of Human Papilloma Virus Type 16 from Cervical Cancer Patient

Background: Cervical cancer is the second most cancer in the world after breast cancer, this cancer is caused by infection of high risk Human Papillomavirus (HPV) type 16. It is often found in cervical cancer whose genome structure is composed of L1 proteins. L1 protein makes up the viral capsid that has an important role to infect the cervical epithelium. Several studies have found the differences in HPV nucleotides variants that lead to changes in amino acids that can disrupt the structure, the nature function of the virus itself, and ultimately lead to changes in biological functions including host immunological recognition. Variation of the L1 gene will also affect the effectiveness of existing vaccines. Methods: : This research is a descriptive study conducted at the laboratory of microbiology at the Faculty of Medicine at Andalas Padang University from February to August 2018 which aims to look at the molecular variations of the L1 HPV type 16 gene and see phylogenic kinship. Results: : This study obtained SNPs (Single Nucleotide Polymorphism) on all HPV 16 samples in the form of C / G (6240), A / G (6432), T / G (6686), C / T (6823) and insertion of nucleotide bases ACT (6901) ) and followed by GAT base deletions (6953) variations occurring along the observed sample isolate sequences. Conclusion: There are molecular variations of the L1 HPV type 16 gene which can cause different host immune responses. Phylogenic kinship of HPV type 16 isolate in Riau is close to Asian-American isolate.