Phytochemical analysis, antioxidant and antimicrobial activity in leaves samples of Bacopa monnieri (L.) Pennell from different geographical regions in India

Background: To determine quantitative analysis of total phenolic compound and flavonoids content and evaluate antioxidant and antibacterial activity in leaves samples of Bacopa monnieri (L.) from four different geographical regions in India. Result: Phytochemical analysis was performed on all four leaves samples and estimation of Total phenolic and flavonoid content were carried out. Evaluation of antioxidant activities such as DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity, hydrogen peroxide (H 2 O 2 ) scavenging activity, ferric reducing power and antibacterial activity in different solvent extracts of leaves samples from four different geographical regions in India were performed. The highest content of total phenolics and flavonoids were observed in leaves sample of Jammu region. Highest DPPH radical scavenging activity, H 2 O 2 scavenging activity and ferric reducing power were observed in leaves samples of Jammu. Maximum zone of inhibition against bacteria Staphylococcus aureus was reported with ethanol extract of leaves sample of Jammu. Maximum zone of inhibition against bacteria Bacillus subtilis was reported with chloroform extract of leaves sample of Kerala. Maximum zone of inhibition against bacteria Pseudomonas aeruginosa was reported with ethanol extract of leaves sample of Jammu. Maximum zone of inhibition against bacteria Escherichia coli was reported with ethanol extract of leaves sample of Kerala. Conclusion: Excellent antioxidant activity was given by extract of leaves samples of Jammu. So they can be used as natural antioxidants after isolation and purification. Excellent antibacterial activity was reported in leaves samples of Jammu and Kerala.