Acidification and Nitrification Inhibition of Manure Alters Greenhouse Gas Emissions and Nitrogen Cycling in Diverse Agricultural Soils

Acidification and Nitrification Inhibition of Manure Alters Greenhouse Gas Emissions and Nitrogen Cycling in Diverse Agricultural Soils | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 25 August 2025 V1 Latest version Share on Acidification and Nitrification Inhibition of Manure Alters Greenhouse Gas Emissions and Nitrogen Cycling in Diverse Agricultural Soils Authors : Mitch Wodrich 0009-0008-0967-8471 , Steven J. Hall 0000-0002-7841-2019 , and Xia Zhu-Barker 0000-0001-9050-3696 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175615710.08972894/v1 Published Journal of Environmental Quality Version of record Peer review timeline 140 views 101 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Manure amendments are widely used in agriculture to enhance crop productivity and maintain soil organic matter, but they also contribute to greenhouse gas (GHG) emissions and reactive nitrogen (N) losses. Manure acidification and nitrification inhibitors (NIs) have been proposed as mitigation strategies, but their effectiveness likely varies with soil characteristics. To evaluate the influence of acidified and NI-treated manure on N cycling across different soil types, we conducted a 28-day laboratory incubation using soils collected from major agricultural regions in Wisconsin. We measured emissions of nitrous oxide (N2O), nitric oxide (NO), carbon dioxide (CO2), and methane (CH4), along with soil ammonium (NH4+) and nitrate (NO3-) dynamics. Acidified manure enhanced short-term NH4⁺ retention in all soils but increased cumulative N2O emissions in the sandy soil compared to untreated manure, likely due to pH-driven disruption of nitrification and incomplete denitrification. In contrast, the NI dicyandiamide (DCD) consistently suppressed nitrification early in the incubation, resulting in significantly lower N2O and NO emissions, often approaching levels observed in the no-manure controls, particularly in the sandy soil. Manure amendments increased CO2 fluxes relative to the no-manure controls, but acidified manure emitted less CO2 than DCD-treated manure, likely due to temporary suppression of microbial respiration. CH4 emissions were minimal and largely unaffected by treatments. NIs offer consistent benefits in reducing N2O and NO losses while acidification can increase these emissions in certain soil conditions, highlighting the importance of tailoring management practices to specific soil characteristics. Supplementary Material File (mitch_incubation_manuscript_8.17.25_final.docx) Download 672.52 KB Information & Authors Information Version history V1 Version 1 25 August 2025 Peer review timeline Published Journal of Environmental Quality Version of Record 14 Dec 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords ghg manure mineralization nitrogen soil properties Authors Affiliations Mitch Wodrich 0009-0008-0967-8471 University of Wisconsin-Madison View all articles by this author Steven J. Hall 0000-0002-7841-2019 University of Wisconsin-Madison View all articles by this author Xia Zhu-Barker 0000-0001-9050-3696 [email protected] University of Wisconsin-Madison View all articles by this author Funding Information Wisconsin Department of Natural Resources Xia Zhu-Barker Metrics & Citations Metrics Article Usage 140 views 101 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Mitch Wodrich, Steven J. Hall, Xia Zhu-Barker. Acidification and Nitrification Inhibition of Manure Alters Greenhouse Gas Emissions and Nitrogen Cycling in Diverse Agricultural Soils. Authorea . 25 August 2025. DOI: https://doi.org/10.22541/au.175615710.08972894/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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