Analysis of 5mC and 5hmC in endometriosis lesions and stroma cells.

Epigenetic Alterations Affecting Transcription Factors and Signaling Pathways in Stromal Cells of Endometriosis Fig 3 Analysis of 5mC and 5hmC in endometriosis lesions and stroma cells. A) Immunofluorescence analysis of the levels of expression of 5mC (green) and 5hmC (red) in tissue samples of women with endometriosis and controls, showing disease-dependent loss of 5hmC in the epithelial but not in the stromal cell compartment. Representative photos from a total number of n = 2 control and n = 2 endometriotic tissue are shown. B) The results of the standard and oxidative bis-seq for BDNF in endometriotic and control stromal cells represented as QUMA plots (left) and as bar graph (right). The QUMA plot and bar-graph show the percent methylation assessed by standard bis-seq (which scores indistinctly 5hmC and 5mC) and by modified bis-seq (which scores 5mC only) in control stroma and endometriotic stroma cells. The contribution of 5hmC in endometriotic and control stroma is inferred from the difference between the percent methylation at each CpG in the standard bis-seq reactions (5hmC + 5mC) and the percent methylation in the modified bis-seq reactions (5mC). C) The data of JAZF1 standard and oxidative bis-seq in endometriotic and control stromal cells represented as QUMA plots (left) and bar-graph (right) are shown.