Isolation, identification and herbicidal potential of Fusarium avenaceum HY-041 from the Qing-Tibetan Plateau

AbstractIn order to screen a biocontrol strain with broad-spectrum and high herbicidal activity, potential herbicidal strain HY-041 isolated from the leaves of naturally diseased Cephalanoplos setosum was tested. Based on the morphological characteristics and gene sequence analysis, the fungus was identified asFusarium avenaceum. Herbicidal activity to 9 target weeds were tested, the results showed that the pathogenicity of strain HY-041 to different weeds invitrowas as follows:Polygonum lapathifolium>Malva verticillata>Avena fatua>Chenopodium album>Polygonum aviculare>Elsholtzia densa>Amaranthus retroflexus>Thlaspi arvense>Galium aparine, the pot experiment showed that the fermentation filtrate was highly pathogenic toC. album,E. densaandA. fatuaby spray inoculation. The safety evaluation results showed that the strain was moderately susceptible toBrassica napusL., slightly infectedTriticum aestivumL. andHordeum vulgare, relatively safe toPisum sativumL. andVicia fabaL.. The scanning electron microscope observation showed that strain HY-041 hyphae destroyed the epidermis tissue, invadedA. fatuatissue from the stoma and parasites, and propagated in the tissue to produce spores. The results showed that strain HY-041 had the potential to develop microbial biocontrol herbicide.