Genome Characterization of the Novel Lytic Genome Sequence of Phage YUEEL01 of the Myoviridae Family

Abstract Antimicrobial resistance is a global concern due to its rapid emergence in the environment and the associated high risk to human and animal health. Municipal wastewater, including urban, hospital, and pharmaceutical effluent, is the main source of antibiotic and antibiotic resistant bacteria (ARB) contamination, and biological processes are commonly used for wastewater treatment. Biologically based strategies seem to be a promising approach to effective integrated ARB control since they can focus on the bases of antibiotic resistance. To develop an effective bacteriophage against multi-drug resistance (MDR) microbes in municipal wastewater, phage YUEEL01 was isolated from a livestock farm, and its genome sequenced. Phenotypic characterization suggested that the phage is a member of the Myoviridae family, and that it encodes 168,266 bp with an overall GC content of 35.4% had 259 putative protein-coding genes, 11 tRNA and six hypothetical genes. A number of putative DNA replication and regulation, DNA packing and structure and host lysis genes were identified. Further, whole genome phylogenetic analysis demonstrated that phage YUEEL01 is closely related to phage slur03. Elucidation of the YUEEL01 phage might be helpful when developing antibacterial tools for controlling MDR bacteria in wastewater treatment systems.