The immunogenicity identification and adherence characterization analysis of acetate kinase of Mycoplasma synoviae

Abstract Background: Mycoplasma synoviae (MS) is a major pathogen that leads to a significant economic loss to the world’s industry of poultry. Acetate kinase (ACK) is a member of the key pathway for acetyl-CoA synthesis in bacteria, but its role in MS maintains unknown. Results: The immunogenicity assay and complement-dependent mycoplasmacidal assay identified recombinant protein MS ACK (rMS ACK) as an immunogenic protein. The distribution of ACK was localized to both the cytoplasm and membrane of MS detected by Western blot and ELISA. Meanwhile, adherence and adherence inhibition assay indicated that the rMS ACK could directly adhere to DF-1 cells, and anti-rMS ACK serum could effectively inhibit the adherence of MS to DF-1 cells. Conclusion: The immunogenicity and adhesion characterization of ACK membrane protein were analyzed for the first time, enhancing the understanding of the pathogenicity of MS infection.