In vitro evaluation of selective cytotoxic activity of Chaerophyllum macropodum Boiss. on cultured human neuroblastoma SH-SY5Y cells

Neuroblastoma is the most common solid tumor in children. New treatment approaches are needed because of the harmful side effects and costs of the methods used in the treatment of neuroblastoma. Medicinal and aromatic plants are important for new treatment approaches due to their minimal side effects and economic advantages. Therefore, the present study was carried out to examine the cytotoxic effect of Chaerophyllum macropodum extract on human neuroblastoma (SH-SY5Y) and fibroblast (HDFa) cell lines. 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and Lactate dehydrogenase release (LDH) assays were used to determine the cytotoxic effect of Chaerophyllum macropodum . The results revealed that Chaerophyllum macropodum had a significant cytotoxic effect on neuroblast cells at all concentrations used (p < 0.05). But it did not show any effect on human fibroblasts. As a result, the obtained data clearly showed that Chaerophyllum macropodum exerts a selective effect on neuroblastoma cells for the first time.