The genome sequence of Vine’s Rustic moth, Hoplodrina ambigua (Denis & Schiffermüller, 1775)

We present a genome assembly from an individual female Hoplodrina ambigua (Vine’s Rustic; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 438.1 megabases in span. Most of the assembly is scaffolded into 32 chromosomal pseudomolecules, including the Z and W sex chromosomes. The mitochondrial genome has also been assembled and is 15.39 kilobases in length. Gene annotation of this assembly on Ensembl identified 18,878 protein coding genes.