ALG3 drives UCEC progression via MYC-dependent fatty acid metabolism

ALG3 drives UCEC progression via MYC-dependent fatty acid metabolism | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article ALG3 drives UCEC progression via MYC-dependent fatty acid metabolism Qian Wu, Tingting Zhang, Mengdie Shi, Qiufen Guo This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7103928/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Uterine corpus endometrial carcinoma (UCEC) is one of the most common tumors in the female reproductive system, which is an important cause of death in women. ALG3 is high expression in UCEC, but it’s molecular mechanism is still unclear. Methods The ALG3 expression in UCEC was analyzed by TCGA-UCEC database. The cell proliferation was detected with CCK-8, EdU and colony formation assays. Cells apoptosis was analyzed by flow cytometry. Wound healing and transwell assays were performed to detect cell migration. The protein levels were analyzed via western blot. The intracellular triglycerides and cholesterol were analyzed by microplate reader. Xenograft mouse models were used to explore the effect of ALG3 on UCEC in vivo . Results ALG3 was highly expressed in UCEC. Patients with high ALG3 expression have poor prognosis. Additionally, the silence of ALG3 inhibited the proliferation and migration of UCEC cells, while promoted apoptosis. However, overexpression of ALG3 produced opposite results. ALG3 knockdown suppressed the MYC level and fatty acid metabolism in UCEC cells. Subsequently, the fatty acid metabolism, cell proliferation, cell migration and cell anti-apoptosis were promoted with MYC overexpressed in UCEC cells. In vivo , the ALG3 knockdown suppressed the progression of UCEC and fatty acid metabolism. Conclusion ALG3 enhanced MYC-mediated fatty acid metabolism to promote UCEC growth and metastasis, and inhibit cell apoptosis. ALG3 could be used as a new therapeutic target for UCEC. UCEC ALG3 MYC Fatty acid metabolism proliferation and migration Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7103928","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":496143637,"identity":"f5e0a9ed-6954-41e4-8593-6651e2a986e3","order_by":0,"name":"Qian Wu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAy0lEQVRIiWNgGAWjYJCCAwwMNvX97A0gBvFa0hhn9hwgQQsQHGbccCOBSLUGN5I3Hvi5I41Zcubzh4cLahjk+cUIWGZwI63gYO8ZGzZ+6RyDwzOOMRjOnE3AOrMbOQYHeNvSeCRn5zAc5mFjSDC4TYSWg3/bDksY3Dz+4DDPPyK1HOZtO2xgcIMBxCBCi/2ZZwWHZdvSEiR7QHr7JAj7RbI9efPHt202Cfzsxx9/5vlmI88vTUALEBggcyQIKsfQMgpGwSgYBaMAEwAAs0VKMGezAbwAAAAASUVORK5CYII=","orcid":"","institution":"Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences","correspondingAuthor":true,"prefix":"","firstName":"Qian","middleName":"","lastName":"Wu","suffix":""},{"id":496143638,"identity":"f52f3863-5f86-4a07-8764-90ca04c95768","order_by":1,"name":"Tingting Zhang","email":"","orcid":"","institution":"Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Tingting","middleName":"","lastName":"Zhang","suffix":""},{"id":496143639,"identity":"ae5fee83-8a0a-4d7a-a920-400f03ff123c","order_by":2,"name":"Mengdie Shi","email":"","orcid":"","institution":"The Affiliated Suzhou Hospital of Nanjing Medical University","correspondingAuthor":false,"prefix":"","firstName":"Mengdie","middleName":"","lastName":"Shi","suffix":""},{"id":496143640,"identity":"f1013c55-99df-4c78-8ca3-dafa5e08f37e","order_by":3,"name":"Qiufen Guo","email":"","orcid":"","institution":"Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Qiufen","middleName":"","lastName":"Guo","suffix":""}],"badges":[],"createdAt":"2025-07-11 18:23:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7103928/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7103928/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":90139301,"identity":"7790a419-16a8-4edb-8494-443ea037337e","added_by":"auto","created_at":"2025-08-29 03:16:53","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1640874,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript2025.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7103928/v1_covered_c9f9f5fc-7955-4119-a3ef-17c74392ed3f.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"ALG3 drives UCEC progression via MYC-dependent fatty acid metabolism","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"UCEC, ALG3, MYC, Fatty acid metabolism, proliferation and migration","lastPublishedDoi":"10.21203/rs.3.rs-7103928/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7103928/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e\u003cp\u003eUterine corpus endometrial carcinoma (UCEC) is one of the most common tumors in the female reproductive system, which is an important cause of death in women. ALG3 is high expression in UCEC, but it\u0026rsquo;s molecular mechanism is still unclear.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e\u003cp\u003eThe ALG3 expression in UCEC was analyzed by TCGA-UCEC database. The cell proliferation was detected with CCK-8, EdU and colony formation assays. Cells apoptosis was analyzed by flow cytometry. Wound healing and transwell assays were performed to detect cell migration. The protein levels were analyzed via western blot. The intracellular triglycerides and cholesterol were analyzed by microplate reader. Xenograft mouse models were used to explore the effect of ALG3 on UCEC \u003cem\u003ein vivo\u003c/em\u003e.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e\u003cp\u003eALG3 was highly expressed in UCEC. Patients with high ALG3 expression have poor prognosis. Additionally, the silence of ALG3 inhibited the proliferation and migration of UCEC cells, while promoted apoptosis. However, overexpression of ALG3 produced opposite results. ALG3 knockdown suppressed the MYC level and fatty acid metabolism in UCEC cells. Subsequently, the fatty acid metabolism, cell proliferation, cell migration and cell anti-apoptosis were promoted with MYC overexpressed in UCEC cells. \u003cem\u003eIn vivo\u003c/em\u003e, the ALG3 knockdown suppressed the progression of UCEC and fatty acid metabolism.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e\u003cp\u003eALG3 enhanced MYC-mediated fatty acid metabolism to promote UCEC growth and metastasis, and inhibit cell apoptosis. ALG3 could be used as a new therapeutic target for UCEC.\u003c/p\u003e","manuscriptTitle":"ALG3 drives UCEC progression via MYC-dependent fatty acid metabolism","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-08-08 20:51:36","doi":"10.21203/rs.3.rs-7103928/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"bf7529d9-efc3-41a2-891e-4a595ef161ae","owner":[],"postedDate":"August 8th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-08-29T03:08:46+00:00","versionOfRecord":[],"versionCreatedAt":"2025-08-08 20:51:36","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7103928","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7103928","identity":"rs-7103928","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}