Confocal laser scanning platform combined with in situ high-resolution quantitative phase imaging

Confocal laser scanning platform combined with in situ high-resolution quantitative phase imaging | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Journal of Biophotonics This is a preprint and has not been peer reviewed. Data may be preliminary. 9 January 2025 V1 Latest version Share on Confocal laser scanning platform combined with in situ high-resolution quantitative phase imaging Authors : Wenjing Feng , Hongfei Suo , ying ma 0000-0002-3105-375X [email protected] , Hanwei Cheng , Peng Gao , Yunze Lei , Sha An , Juanjuan Zheng , and Min Liu Authors Info & Affiliations https://doi.org/10.22541/au.173640928.87823222/v1 Published Journal of Biophotonics Version of record Peer review timeline 332 views 191 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract This paper introduces an advanced dual-modality imaging platform by integrating a confocal laser scanning/imaging module with flat-fielding quantitative phase contrast microscopy (FF-QPCM). The platform enables phase/fluorescence dual-modality imaging of the same samples with high resolution and contrast. Using this platform, we observed that confocal fluorescence excitation causes minimal damage to cells, whereas wide-field fluorescence excitation results in significant damage, particularly mitochondrial fragmentation and notable alterations in phase values. Additionally, in situ quantitative phase imaging was conducted on live COS7 cells with a specific region irradiated by a confocal laser. The results demonstrated that 5-minute continuous confocal laser irradiation affects only COS7 cells with fluorescently labeled mitochondria, causing mitochondrial dysfunction throughout the cell. These findings suggest that integrating FF-QPCM with a confocal laser scanning/imaging module offers a highly efficient, sensitive, and information-rich tool for cell biology research, with broad application potential. Supplementary Material File (confocal laser scanning platform combined with in situ high-resolution quantitative phase imaging.docx) Download 5.11 MB Information & Authors Information Version history V1 Version 1 09 January 2025 Peer review timeline Published Journal of Biophotonics Version of Record 7 Mar 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Journal of Biophotonics Keywords confocal laser dual-modality imaging in situ detection live cell quantitative phase Authors Affiliations Wenjing Feng Xidian University View all articles by this author Hongfei Suo Xidian University View all articles by this author ying ma 0000-0002-3105-375X [email protected] Xidian University View all articles by this author Hanwei Cheng Xidian University View all articles by this author Peng Gao Xidian University View all articles by this author Yunze Lei Xidian University View all articles by this author Sha An Xidian University View all articles by this author Juanjuan Zheng Xidian University View all articles by this author Min Liu Xidian University View all articles by this author Metrics & Citations Metrics Article Usage 332 views 191 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Wenjing Feng, Hongfei Suo, ying ma, et al. Confocal laser scanning platform combined with in situ high-resolution quantitative phase imaging. Authorea . 09 January 2025. DOI: https://doi.org/10.22541/au.173640928.87823222/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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