A universal microscope add-on for snapshot quantitative phase imaging by spectral encoding

A universal microscope add-on for snapshot quantitative phase imaging by spectral encoding | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article A universal microscope add-on for snapshot quantitative phase imaging by spectral encoding Jinli Suo, Weihang Zhang, Xin Liu, Wenxuan Miao, Hai Qi, Qionghai Dai This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4420203/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Observing the dynamics of colorless or transparent samples quantitatively is vital in cellular-level biomedical research. Among existing techniques, fluorescent microscopy suffers from phototoxicity and photobleaching issues, while snapshot quantitative phase imaging (QPI) demands a complicated setup. Thus, an easily accessible tool allowing for long-period, high-contrast observation of transparent samples remains still lacking. To tackle this problem, we propose a one-shot QPI approach compatible with both off-the-shelf and customized microscopes, which encodes the phase informative cues into a snapshot using a thin, flat optical device and decodes the phase information through an advanced deep network algorithm. The encoding involves overlaying defocused images obtained through a wafer-thin highly-dispersive glass with dual-bandpass coating, acting as an ``add-on'' module located between the specimen and objective. The successive phase retrieval is conducted with a well-designed network structure and the high-quality training data supports the phase reconstruction at high axial resolution. By experimenting on T cell migration, T cell activation, and real-time live-cell drug screening on systems with various magnifications, we demonstrate high-precision phase retrieval of our method under different experimental settings and on various platforms, which is expected to obtain a wide range of potential applications in studies of clustered cell morphology, cell counting, and cell migration tracking. Full Text Additional Declarations (Not answered) Supplementary Files SupplDoc.pdf Tcellmigration.avi Supplementary Video 1 Tcellactivation.avi Supplementary Video 2 drugscreening.avi Supplementary Video 3 Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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