Evaluating Experimental Design to Sample Mosquitoes

Evaluating Experimental Design to Sample Mosquitoes | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Evaluating Experimental Design to Sample Mosquitoes Heloise Rozier, Pierre Gloaguen, François Septier, Sébastien Boyer This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6704646/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Dec, 2025 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Mosquito study is crucial for public health, research, and vector control. We evaluated four understudiedaspects of experimental design: (i) global efficiency of trapping devices - BG-Sentinel (BG) vs. CDC lighttraps (LT)), (ii) temporal efficiency over a three-day sampling period, (iii) the impact of sampling durationon vector presence and abundance assessments, and (iv) site visit frequency for biodiversity surveys. Bothtraps were deployed across 10 Cambodian provinces, with collections conducted every 24 hours over three-dayperiods from 2019 to 2021. A total of 1,992 collections yielded 181,798 mosquitoes spanning 153 identifiedspecies. Using a hurdle generalized linear mixed model to address overdispersion and zero inflation, we foundthat trap type significantly influenced 21 of 54 studied species: LT captured 15 species more efficiently, while6 species preferred BG. Anthropophilic vectors such as Aedes aegypti and Aedes albopictus were moreattracted to BG, whereas LT captured higher species richness and zoophilic vectors. Capture efficiencydeclined over time for 6 of 13 primary vector species. However, annual trends in presence and abundanceremained consistent regardless of whether sampling lasted one, two, or three days. Finally, we quantifiedthe probability of collecting a new species during subsequent missions, showing that this probability declineswith visit sampling effort at rates dependent on trap type and sampling days duration. These findingsprovide empirical guidelines for optimizing mosquito surveillance strategies based on study objectives. Earth and environmental sciences/Ecology/Population dynamics Earth and environmental sciences/Environmental sciences Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 10 Dec, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 05 Aug, 2025 Reviews received at journal 30 Jun, 2025 Reviews received at journal 30 Jun, 2025 Reviewers agreed at journal 04 Jun, 2025 Reviewers agreed at journal 04 Jun, 2025 Reviewers invited by journal 03 Jun, 2025 Editor assigned by journal 03 Jun, 2025 Editor invited by journal 02 Jun, 2025 Submission checks completed at journal 27 May, 2025 First submitted to journal 27 May, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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