Non-invasive imaging of salicylic and jasmonic acid activities in planta

2025 · doi:10.1101/2025.02.17.636591
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Abstract

Jasmonic and salicylic acids are the major hormones involved in plant response to pests and pathogens. Here, we engineered autoluminescent plants that report activity of these hormones with up to 53-fold contrast. Using consumer-grade cameras, we imaged reporter Arabidopsis thaliana and Nicotiana benthamiana plants throughout normal development, and in response to attacks of pests and pathogens.
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New Results Non-invasive imaging of salicylic and jasmonic acid activities in planta Anastasia V Balakireva, Tatiana A Karataeva, Michael Karampelias, Tatiana Yu Mitiouchkina, Jan Macháček, Ekaterina S Shakhova, Maxim M Perfilov, Olga A Belozerova, Kseniia A Palkina, Nikola Drazna, Zuzana Vondrakova, View ORCID ProfileAubin Fleiss, Liliia I Fakhranurova, Nadezhda M Markina, Viktor V Morozov, Evgenia N Bugaeva, Galina M Delnova, Vladimir V Choob, Ilia V Yampolsky, Jan Petrášek, View ORCID ProfileAlexander S Mishin, View ORCID ProfileKaren S Sarkisyan doi: https://doi.org/10.1101/2025.02.17.636591 Anastasia V Balakireva 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Tatiana A Karataeva 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Michael Karampelias 3Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02, Prague 6, Czech Republic Tatiana Yu Mitiouchkina 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Jan Macháček 3Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02, Prague 6, Czech Republic Ekaterina S Shakhova 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Maxim M Perfilov 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Olga A Belozerova 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Kseniia A Palkina 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Nikola Drazna 3Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02, Prague 6, Czech Republic Zuzana Vondrakova 3Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02, Prague 6, Czech Republic Aubin Fleiss 4Synthetic Biology Group, MRC London Institute of Medical Sciences, London, UK 5Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK Liliia I Fakhranurova 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Nadezhda M Markina 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Viktor V Morozov 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Evgenia N Bugaeva 1Planta LLC, 121205 Moscow, Russia Galina M Delnova 1Planta LLC, 121205 Moscow, Russia Vladimir V Choob 1Planta LLC, 121205 Moscow, Russia 6Botanical Garden of Lomonosov Moscow State University, Vorobievy Gory 1 b.12, Moscow 119234 Russia Ilia V Yampolsky 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia 7Pirogov Russian National Research Medical University, Ostrovityanova 1, Moscow 117997, Russia 8Light Bio Inc, Ketchum, Idaho, USA Jan Petrášek 3Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02, Prague 6, Czech Republic Alexander S Mishin 1Planta LLC, 121205 Moscow, Russia 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia Karen S Sarkisyan 4Synthetic Biology Group, MRC London Institute of Medical Sciences, London, UK 5Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK 8Light Bio Inc, Ketchum, Idaho, USA Abstract Jasmonic and salicylic acids are the major hormones involved in plant response to pests and pathogens. Here, we engineered autoluminescent plants that report activity of these hormones with up to 53-fold contrast. Using consumer-grade cameras, we imaged reporter Arabidopsis thaliana and Nicotiana benthamiana plants throughout normal development, and in response to attacks of pests and pathogens. Competing Interest Statement This study was partially funded by Planta (planta.bio) and Light Bio (light.bio). Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. Posted February 17, 2025. Non-invasive imaging of salicylic and jasmonic acid activities in planta Anastasia V Balakireva, Tatiana A Karataeva, Michael Karampelias, Tatiana Yu Mitiouchkina, Jan Macháček, Ekaterina S Shakhova, Maxim M Perfilov, Olga A Belozerova, Kseniia A Palkina, Nikola Drazna, Zuzana Vondrakova, Aubin Fleiss, Liliia I Fakhranurova, Nadezhda M Markina, Viktor V Morozov, Evgenia N Bugaeva, Galina M Delnova, Vladimir V Choob, Ilia V Yampolsky, Jan Petrášek, Alexander S Mishin, Karen S Sarkisyan bioRxiv 2025.02.17.636591; doi: https://doi.org/10.1101/2025.02.17.636591 Non-invasive imaging of salicylic and jasmonic acid activities in planta Anastasia V Balakireva, Tatiana A Karataeva, Michael Karampelias, Tatiana Yu Mitiouchkina, Jan Macháček, Ekaterina S Shakhova, Maxim M Perfilov, Olga A Belozerova, Kseniia A Palkina, Nikola Drazna, Zuzana Vondrakova, Aubin Fleiss, Liliia I Fakhranurova, Nadezhda M Markina, Viktor V Morozov, Evgenia N Bugaeva, Galina M Delnova, Vladimir V Choob, Ilia V Yampolsky, Jan Petrášek, Alexander S Mishin, Karen S Sarkisyan bioRxiv 2025.02.17.636591; doi: https://doi.org/10.1101/2025.02.17.636591 Subject Area Subject Areas - Biochemistry (17705) - Bioengineering (13899) - Bioinformatics (41968) - Biophysics (21460) - Cancer Biology (18603) - Cell Biology (25526) - Clinical Trials (138) - Developmental Biology (13385) - Ecology (19910) - Epidemiology (2067) - Evolutionary Biology (24328) - Genetics (15614) - Genomics (22513) - Immunology (17741) - Microbiology (40423) - Molecular Biology (17193) - Neuroscience (88646) - Paleontology (667) - Pathology (2835) - Pharmacology and Toxicology (4827) - Physiology (7647) - Plant Biology (15160) - Synthetic Biology (4302) - Systems Biology (9825) - Zoology (2271)

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