An Enduring In vitro Wound Healing Phase Recipient by Bioactive Glass- Graphene Oxide Nanocomposites

Bioactive glass (BG) is intensely interested in soft tissue engineering because of its biocompatibility and bonding potential to increase fibroblast cell proliferation, growth factors, and granulation tissue development. The proposed BG with and without sodium (Na) was prepared using the sol-gel method for wound healing studies. The BG/graphene oxide (GO) and BG (Na-free)/GO nanocomposites were investigated against fibroblast L929 cells in vitro ; the 45S5 BG nanocomposites exhibited desired cell viability (80%), cell proliferation (30%), cell migration (25%), metabolic activity, and wound contraction due to extracellular matrix (ECM) production and enhanced protein release by fibroblast cells. Additionally, the antioxidant assays for BG, BG (Na-free), GO, and BG/GO, BG (Na-free)/GO were evaluated for effective wound healing with reduced inflammation sites on the wound area, which was assessed by the (2,2-diphenyl-1-picryl-hydrazyl-hydrate) (DPPH) assay with 80% radical scavenging activity, confirming their anti-inflammation and improved wound healing properties.