Molecular mechanism and biological function of miRNA-155 and its target genes on endometriosis

Objective:To explore molecular mechanism and biological function of miR-155 and its target genes on endometriosis.Methods:The expression of miR-155 in endometriosis(Ems)patient and healthy control was assayed by RT-PCR.After miR-155 mimic and inhibtor were transfected into Ems endometrial cells for 48 h,the viability of cell was detected by MTT assay.Transwell migration and invasion assay were used to detect cell migration and invasion.The expression of cell apoptotic protein Bax and Bcl-2,matrix metalloproteinase(MMP 2)and MMP 9was assayed by western blot.Results:The expression of miR-155 in Ems patient was more than that in health control(P0.01).After miR-155 mimic and inhibitor were transfected into Ems endometrial cells for 48 h,miR-155over-expression could increase cell viability,and promote cell migration and invasion,which was related to down-regulation of Bax along with up-regulation of Bcl-2,MMP 2and MMP 9.Conclusion:These results suggests that miR-155 lower expression can inhibit endometrial cell proliferation and migration of the Ems.