Fluoride ingestion induces formation of unusual macromolecular complexes in gut lumen which retard absorption of essential minerals and trace elements by chelation

Fluoride ingestion induces formation of unusual macromolecular complexes in gut lumen which retard absorption of essential minerals and trace elements by chelation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Fluoride ingestion induces formation of unusual macromolecular complexes in gut lumen which retard absorption of essential minerals and trace elements by chelation Saba Sarwar, Javed Ahsan Quadri, A Shariff This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4655131/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 08 Oct, 2024 Read the published version in Biological Trace Element Research → Version 1 posted 10 You are reading this latest preprint version Abstract This research study aimed to elucidate the impact of chronic fluoride exposure on absorption of essential minerals in gut and to decipher the mechanism of mineral deficiency due to fluoride ingestion. Male Wistar rats were randomly selected and divided in to three groups. Group-1 (G-1) was taken as control (non-fluoride exposed), while group-2 & group-3 (G-2&G-3) were administered a human equivalent dose of fluoride (50 and 100 ppm ad-libitum, HED=5 & 10 ppm in human, respectively) for 75-days. Serum fluoride concentrations were measured. Blood and stool concentration of essential minerals and trace elements were measured by ICP-MS. Chemical compounds present in the stool were also analysed by XRD. The chemical compounds and macromolecular complexes having fluoride and essential minerals were identified and quantified by Match3 software. In the fluoride exposed groups, the blood concentrations of essential minerals were significantly (≤0.05) lower than the control. While excretion of essential elements in stool were significantly higher in fluoride administered groups (≤0.05) compared to control group. The XRD analysis of stool showed formation of various unusual macromolecular chemical complexes in fluoride treated groups and the types and concentrations of these compounds increased with increasing fluoride dosage. The presence of fluoride in stomach, chelates minerals and reduced absorption. The fluoride induces formation of unusual high molecular weight macromolecular chelation complexes change the chemical species in the gut and the absorption of essential minerals reduced. Fluoride toxicity Essential minerals Trace elements Mineral absorption in gut Macromolecular complexes Elemental chelation in the gut lumen Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 08 Oct, 2024 Read the published version in Biological Trace Element Research → Version 1 posted Editorial decision: Revision requested 23 Aug, 2024 Reviews received at journal 13 Aug, 2024 Reviews received at journal 08 Aug, 2024 Reviewers agreed at journal 31 Jul, 2024 Reviewers agreed at journal 30 Jul, 2024 Reviewers agreed at journal 30 Jul, 2024 Reviewers invited by journal 04 Jul, 2024 Editor assigned by journal 30 Jun, 2024 Submission checks completed at journal 30 Jun, 2024 First submitted to journal 28 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4655131","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":324908995,"identity":"e2c2d625-d568-447b-bf57-c028f81e43b5","order_by":0,"name":"Saba Sarwar","email":"","orcid":"","institution":"AIIMS","correspondingAuthor":false,"prefix":"","firstName":"Saba","middleName":"","lastName":"Sarwar","suffix":""},{"id":324908996,"identity":"239ba6a1-5918-4ee7-9b1f-a3c5ee52d547","order_by":1,"name":"Javed Ahsan Quadri","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA20lEQVRIiWNgGAWjYBACxgYgwcMgwcPAwHwAyJSQIUULWwJICw9xVkGU8Rgg2PgAc/sZwwdvaixkdPvPfH51o8aCh4H98NENeB3Wk2NsOOeYBI/Zjdxt1jlABgNPWtoN/H5JS5PmYQNp4d1mnANkMIDYeLX0P0v/zfMPqOz8mWfGOf+I0TIj+RgzbxtQ2YEc5se5bURpeXxYcm4fSFmaGXMukMFGyC+G/YmNH958q7M3O3/48eecb3Vy/OyHj+HX0oBgs0mASXzKQUAeic38gZDqUTAKRsEoGJkAAIukRGduS2eZAAAAAElFTkSuQmCC","orcid":"","institution":"AIIMS","correspondingAuthor":true,"prefix":"","firstName":"Javed","middleName":"Ahsan","lastName":"Quadri","suffix":""},{"id":324908997,"identity":"d4a63b06-928e-4e07-80d8-9a0aa0e4155b","order_by":2,"name":"A Shariff","email":"","orcid":"","institution":"AIIMS","correspondingAuthor":false,"prefix":"","firstName":"A","middleName":"","lastName":"Shariff","suffix":""}],"badges":[],"createdAt":"2024-06-28 13:37:04","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4655131/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4655131/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s12011-024-04403-1","type":"published","date":"2024-10-08T15:57:41+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":66597191,"identity":"b39a0562-8457-48f1-b9f0-fb4fc2bc92e0","added_by":"auto","created_at":"2024-10-14 16:08:14","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2307536,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptGutChemistryFinal.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4655131/v1_covered_bdcee159-7b6c-44b7-9e06-5123a86e464b.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Fluoride ingestion induces formation of unusual macromolecular complexes in gut lumen which retard absorption of essential minerals and trace elements by chelation","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"biological-trace-element-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bter","sideBox":"Learn more about [Biological Trace Element Research](https://www.springer.com/journal/12011)","snPcode":"12011","submissionUrl":"https://submission.nature.com/new-submission/12011/3","title":"Biological Trace Element Research","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Fluoride toxicity, Essential minerals, Trace elements, Mineral absorption in gut, Macromolecular complexes, Elemental chelation in the gut lumen","lastPublishedDoi":"10.21203/rs.3.rs-4655131/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4655131/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis research study aimed to elucidate the impact of chronic fluoride exposure on absorption of essential minerals in gut and to decipher the mechanism of mineral deficiency due to fluoride ingestion. Male Wistar rats were randomly selected and divided in to three groups. Group-1 (G-1) was taken as control (non-fluoride exposed), while group-2 \u0026amp; group-3 (G-2\u0026amp;G-3) were administered a human equivalent dose of fluoride (50 and 100 ppm ad-libitum, HED=5 \u0026amp; 10 ppm in human, respectively) for 75-days. Serum fluoride concentrations were measured. Blood and stool concentration of essential minerals and trace elements were measured by ICP-MS. Chemical compounds present in the stool were also analysed by XRD. The chemical compounds and macromolecular complexes having fluoride and essential minerals were identified and quantified by Match3 software. In the fluoride exposed groups, the blood concentrations of essential minerals were significantly (≤0.05) lower than the control. While excretion of essential elements in stool were significantly higher in fluoride administered groups (≤0.05) compared to control group. 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