Multi-Lineage Human Endometrial Organoids on Acellular Amniotic Membrane for Endometrium Regeneration

Yuhui Xu, Shuyan Cai, Qian Wang, Minzhang Cheng, Xianrui Hui, Emmanuel Enoch Dzakah, Bing Zhao, Xiaojun Chen
In: Cell Transplantation · 2023 · vol. 32 , pp. 9636897231218408 · doi:10.1177/09636897231218408 · PMID:38097275 · PMC10725651 · W4389751586
article OA: gold CC0 ⤵ 4 in-corpus citations
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study developed multi-lineage human endometrial organoids on acellular amniotic membrane, demonstrating efficacy in promoting endometrial angiogenesis, regeneration, and improving pregnancy outcomes in a rat injury model.

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Abstract

Asherman's syndrome is an endometrial regeneration disorder resulting from injury to the endometrial basal layer, causing the formation of scar tissue in the uterus and cervix. This usually leads to uterine infertility, menstrual disorders, and placental abnormalities. While stem cell therapy has shown extensive progress in repairing the damaged endometrium and preventing intrauterine adhesion, issues of low engraftment rates, rapid senescence, and the risk of tumorigenesis remain to be resolved for efficient and effective application of this technology in endometrial repair. This study addressed these challenges by developing a co-culture system to generate multi-lineage endometrial organoids (MLEOs) comprising endometrial epithelium organoids (EEOs) and endometrial mesenchymal stem cells (eMSCs). The efficacy of these MLEOs was investigated by seeding them on a biocompatible scaffold, the human acellular amniotic membrane (HAAM), to create a biological graft patch, which was subsequently transplanted into an injury model of the endometrium in rats. The results indicated that the MLEOs on the HAAM patch facilitated endometrial angiogenesis, regeneration, and improved pregnancy outcomes. The MLEOs on the HAAM patch could serve as a promising strategy for treating endometrial injury and preventing Asherman's syndrome.

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infertility

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europepmc
last seen: 2026-06-11T06:19:48.454388+00:00
openalex
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License: CC0 · commercial use OK