Evaluating symbiotic N fixation in a hairy vetch breeding program: the importance of phenology and developmental stage

Evaluating symbiotic N fixation in a hairy vetch breeding program: the importance of phenology and developmental stage | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 29 April 2025 V1 Latest version Share on Evaluating symbiotic N fixation in a hairy vetch breeding program: the importance of phenology and developmental stage Authors : Katherine Muller 0000-0001-9144-3867 [email protected] , Lisa Kissing-Kucek , and Laurie Drinkwater Authors Info & Affiliations https://doi.org/10.22541/au.174595468.85912282/v1 186 views 135 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Hairy vetch (Vicia villosa Roth) is an over-wintering annual legume valued for its ability to provide N through symbiotic N fixation (SNF). Although SNF is a high priority trait for farmers, evaluating SNF in a breeding program is a challenging endeavor. Our objective was to evaluate how SNF varies within and among hairy vetch genotypes, and how SNF covaries with traits directly targeted by breeders (i.e., vigor and flowering time). We evaluated SNF in two hairy vetch breeding nurseries (2017 and 2019) based on the proportion of N derived from SNF (Ndfa) measured using the δ15N natural abundance technique. We also considered plant size (vigor) and flowering stage. In 2017 we sampled plants on the same day, at varying developmental stages. In 2019 we sampled plants at a common developmental stage (early flowering) on different days. The 2017 data could not be confidently interpreted because of confounding effects between developmental stage and genotype. The 2019 data collection controlled for developmental stage effects and showed a positive relationship between vigor and Ndfa that appeared to be driven by flowering time. In other words, genotypes with delayed anthesis produced greater biomass and newly fixed N. All three traits showed discernable variation among genotypes and likely reflect cold late winter/early spring conditions that limited growth and N-fixation of early-flowering plants. Taken together, our study shows that developmental stage and community composition are important considerations for evaluating SNF in annual legume breeding programs. Supplementary Material File (bbpaperdraftintemplate_v07.pdf) Download 1.25 MB File (supplement_bbpaper_v05.pdf) Download 1.08 MB Information & Authors Information Version history V1 Version 1 29 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords 15n natural abundance green manure legume nitrogen open pollinated Authors Affiliations Katherine Muller 0000-0001-9144-3867 [email protected] USDA-ARS Northeast Area View all articles by this author Lisa Kissing-Kucek USDA-ARS US Dairy Forage Research Center View all articles by this author Laurie Drinkwater Cornell University CALS View all articles by this author Metrics & Citations Metrics Article Usage 186 views 135 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Katherine Muller, Lisa Kissing-Kucek, Laurie Drinkwater. Evaluating symbiotic N fixation in a hairy vetch breeding program: the importance of phenology and developmental stage. Authorea . 29 April 2025. DOI: https://doi.org/10.22541/au.174595468.85912282/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); Cited by Atsushi Matsumura, Moe Otani, Hiroyuki Daimon, Relay intercropping of hairy vetch green manure into broccoli–sweet corn cropping system: Fertilizer effects on sweet corn growth, Agronomy Journal, 118 , 2, (2026). https://doi.org/10.1002/agj2.70361 Crossref Loading... View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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