In vivo toxicity of industrial biocide containing 2,2-Dibromo-3-nitrilopropionamide in adult and zebrafish larvae

This experimental study examined the effects of a biocide containing 2,2-dibromo-3-nitrilopropionamide (DBNPA) on 120 fertilized eggs and 77 adult zebrafish. The embryos and adults were exposed to a control group and incremental concentrations of DBNPA. The LC50 was determined after exposure and the data were analyzed by probit analysis. In the 5 and 10 ppm dilutions, some symptoms such as spinal deformity, lack of eye formation, and lack of heartbeat indicated the developmental alterations induced by DBNPA in zebrafish larvae. At a dilution of 100 ppm, all eggs died, showing the high toxicity of this concentration. However, no adverse effects on the growth and health of the larvae were observed in lower dilutions. Regarding hatchability, there was no difference between the 0.1 ppm dilution and the control group, but the hatching rate of eggs decreased with increasing DBNPA concentration.