Characterization of Acoerela AcoRL-600 Reference Liposomes using Analytical Ultracentrifugation

Characterization of Acoerela AcoRL-600 Reference Liposomes using Analytical Ultracentrifugation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Characterization of Acoerela AcoRL-600 Reference Liposomes using Analytical Ultracentrifugation Reece Anthony Martin, Borries Demeler This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9003923/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The increasing use of liposomes and lipid nanoparticles (LNPs) for drug delivery requires comprehensive characterization of LNP properties in a physiological solution environment to ensure efficacy and patient safety. This study focuses on commercially available Acoerela AcoRL-600 ™ reference liposomes , which are used to to optimize instrument settings and provide a reliable reference for fluorescence-based analyses of LNPs such as extracellular vesicles (Evs) and lipid-based therapeutics. Analytical ultracentrifugation (AUC) experiments were performed using three detection modes: UV/visible absorbance optics in intensity mode, Rayleigh interference and fluorescence emission. The results show that caution must be exercised when interpreting fluorescence data acquired for liposomes as the smaller particles may not be properly represented due to the effects of quenching. This finding applies not just to AUC experiments, but should also be considered for flow cytometry using fluorescent dyes to label LNPs, liposomes and extracellular vesicles. Analytical ultracentrifugation UltraScan liposomes reference liposomes Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementalAcoerelaManuscriptv827FEB26.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9003923","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":604923242,"identity":"041a4d21-23ea-487f-868d-b0a3bc449f3f","order_by":0,"name":"Reece Anthony Martin","email":"","orcid":"","institution":"University of Lethbridge","correspondingAuthor":false,"prefix":"","firstName":"Reece","middleName":"Anthony","lastName":"Martin","suffix":""},{"id":604923244,"identity":"faf28105-163b-49c8-b947-89e4021e4963","order_by":1,"name":"Borries Demeler","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAxklEQVRIiWNgGAWjYDCCA0D8gIFBjoGdgYGZeC0JDAzGQPWMzSRpSWwgWgvf8e7EBwkV99I3HGY+/riAwU6eoBbJM2c3GyScKc7dcJgtsXkGQ7JhAyEtBjdyt0kktiUAtfAYNvMwHGAkrOX+2+0/Ev8lpBsc5v8I0mJPhC2824B+T0gwOMzDCNKSSFCL5JnczRIJxxIMZx5mM5zNY5CcTFAL3/GzGz98qEmQ5zve/OAzT4WdLUEt6O4kUf0oGAWjYBSMAuwAAGw5QPFDvISBAAAAAElFTkSuQmCC","orcid":"","institution":"University of Lethbridge","correspondingAuthor":true,"prefix":"","firstName":"Borries","middleName":"","lastName":"Demeler","suffix":""}],"badges":[],"createdAt":"2026-03-01 21:25:53","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-9003923/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9003923/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":104783280,"identity":"3c20ca0c-370f-4c4f-b0bd-452517e3b4c1","added_by":"auto","created_at":"2026-03-17 07:58:31","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":464775,"visible":true,"origin":"","legend":"","description":"","filename":"AcoerelaManuscriptsubmitted.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9003923/v1_covered_c59f3e5d-bde8-4435-8027-aad4c5bc0892.pdf"},{"id":104585838,"identity":"bac36b9e-db5d-466b-9c77-0c72733845ef","added_by":"auto","created_at":"2026-03-13 15:47:13","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":608579,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementalAcoerelaManuscriptv827FEB26.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9003923/v1/3642e5e73ae746be1421f674.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Characterization of Acoerela AcoRL-600 Reference Liposomes using Analytical Ultracentrifugation","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Analytical ultracentrifugation, UltraScan, liposomes, reference liposomes","lastPublishedDoi":"10.21203/rs.3.rs-9003923/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9003923/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e \u003cspan type=\"SmallCaps\" class=\"SmallCaps\" name=\"Emphasis\"\u003eThe increasing use of liposomes and lipid nanoparticles (LNPs) for drug delivery requires comprehensive characterization of LNP properties in a physiological solution environment to ensure efficacy and patient safety. This study focuses on commercially available Acoerela AcoRL-600\u003c/span\u003e \u003csup\u003e \u003cspan type=\"SmallCaps\" class=\"SmallCaps\" name=\"Emphasis\"\u003e\u0026trade;\u003c/span\u003e \u003c/sup\u003e \u003cspan type=\"SmallCaps\" class=\"SmallCaps\" name=\"Emphasis\"\u003ereference liposomes\u003c/span\u003e, which are used to to optimize instrument settings and provide a reliable reference for fluorescence-based analyses of LNPs such as extracellular vesicles (Evs) and lipid-based therapeutics. \u003cspan type=\"SmallCaps\" class=\"SmallCaps\" name=\"Emphasis\"\u003eAnalytical ultracentrifugation (AUC) experiments were performed using three detection modes: UV/visible absorbance optics in intensity mode, Rayleigh interference and fluorescence emission. The results show that caution must be exercised when interpreting fluorescence data acquired for liposomes as the smaller particles may not be properly represented due to the effects of quenching. 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