De Novo Transcriptome Assembly Reveals Insights into Osmoregulation and Oxidative Stress Response in the Gills of the Southern King Crab (Lithodes santolla)

De Novo Transcriptome Assembly Reveals Insights into Osmoregulation and Oxidative Stress Response in the Gills of the Southern King Crab (Lithodes santolla) | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 25 April 2025 V1 Latest version Share on De Novo Transcriptome Assembly Reveals Insights into Osmoregulation and Oxidative Stress Response in the Gills of the Southern King Crab (Lithodes santolla) Authors : Alexandra Brante 0009-0007-8645-6314 , Paulina Bustos , Claudio Ortega Muñoz , Eliana Paola Acuña Gomez , Vicenzo Brante , and Rodolfo Farlora [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174558973.34867524/v1 Published Ecology and Evolution Version of record Peer review timeline 208 views 109 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Understanding the molecular mechanisms underlying physiological adaptations in marine species is crucial for assessing their resilience to environmental stressors. The Southern King Crab (Lithodes santolla), an ecologically and commercially important species in sub-Antarctic waters, inhabits dynamic fjord ecosystems characterized by fluctuating salinity and oxygen levels. However, the molecular basis of its adaptive responses remains largely unexplored. In this study, we assembled the first de novo transcriptome of L. santolla to characterize gene expression related to osmoregulation and oxidative stress. Gill samples from individuals collected at two sites: Ballena Sound and Choiseul Bay in the Strait of Magellan (Punta Arenas, Chile), exhibiting different environmental conditions. Sequencing yielded 731,879,912 clean reads, which were assembled into 210,093 transcripts, of which 56,064 contigs successfully annotated. Differential expression analysis identified 4,474 differentially expressed genes (DEGs), with individuals from Ballena Sound exhibiting upregulation of heat shock proteins (HSPs) and Na+/K+-ATPase (NKA), suggesting an osmotic stress response. Conversely, individuals from Choiseul Bay showed increased expression of Na+/H+ exchanger (NHE), V-type proton ATPase (VPA), and antioxidant enzymes, indicating regulatory strategies for salinity adaptation. Gene Ontology and KEGG enrichment analyses further highlighted key pathways involved in ion transport, oxidative stress mitigation, and energy metabolism. This study enhances our understanding of L. santolla’s physiological plasticity and provides a valuable molecular framework for future research on environmental resilience and conservation of sub-Antarctic marine species. Supplementary Material File (brante_et_al_manuscript_me.docx) Download 2.03 MB Information & Authors Information Version history V1 Version 1 25 April 2025 Peer review timeline Published Ecology and Evolution Version of Record 5 Nov 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords lithodes santolla osmoregulation oxidative stress sub-antarctic environment transcriptomics Authors Affiliations Alexandra Brante 0009-0007-8645-6314 Universidad de Valparaíso View all articles by this author Paulina Bustos Universidad de Valparaíso View all articles by this author Claudio Ortega Muñoz Universidad de Valparaíso View all articles by this author Eliana Paola Acuña Gomez Centro de Estudios del Cuaternario de Fuego Patagonia y Antartica View all articles by this author Vicenzo Brante Universidad de Valparaíso View all articles by this author Rodolfo Farlora [email protected] Universidad de Valparaíso View all articles by this author Metrics & Citations Metrics Article Usage 208 views 109 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Alexandra Brante, Paulina Bustos, Claudio Ortega Muñoz, et al. De Novo Transcriptome Assembly Reveals Insights into Osmoregulation and Oxidative Stress Response in the Gills of the Southern King Crab (Lithodes santolla). Authorea . 25 April 2025. DOI: https://doi.org/10.22541/au.174558973.34867524/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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