Perspectives of Lipid Extraction By Biomass Stages of Microalgae Aphanothece Halophytica Developed in Business Scale

Worldwide, people are accepting a sustainable, renewable and economical source for biofuel production without affecting the environmental concerns. Microalgae can overcome problems with reasonable solutions along with higher lipid content, carbon neutrality, biomass productivity and utilization of feed supplements. Picking a distinctive strain is vital and the value depends on its micro, macro nutrients and its photosynthesis efficiency. Extracting oil from the different phases of microalgal biomass is a tedious process. Presently, halophilic Aphanothece halophytica was mass cultivated in raceway ponds using organic and inorganic nutrients. Microalgal biomass was harvested using novel organic flocculant and oil extraction was done through wet and dry basis based on solvent polarity. The process parameters were optimized for maximum lipid productivity using RSM and the empirical model was significantly analysed by analysis of variance. In wet basis, maximum lipid yield was 29.3%, where the reaction temperature, reaction time, biomass-to-solvent ratio and mixing intensity are 68ºC, 190 min, 9:1 and 300 rpm respectively. In dry basis, lipid yield 27.5% was achieved using 12:1 biomass-to-solvent ratio and mixing intensity was 300 rpm for 190 min at 68ºC. Then, the lipid was characterized by GCMS to identify the fatty acid composition to identify the right combination of fatty acid profile for further studies.