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by claude@2026-07, 2026-07-04
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This cross-sectional study investigated genotypic antibiotic-resistance genes in cultured bacterial pathogens isolated from 294 at-risk hospitalized neonates with sepsis in Lagos, using agar culture with identification (including MICROBACT), Kirby-Bauer disc diffusion, and molecular methods (PCR, RAPD-PCR, sequencing) plus docking. Among 110 culture-positive samples, predominant organisms included multiple Gram-negative bacteria (e.g., Klebsiella pneumoniae, Enterobacter gergoviae, Serratia rubidiae, Cronobacter sakazakii) and some Gram-positive species, and most isolates showed resistance to more than three antibiotic classes. Resistance-associated beta-lactam genes bla-CTX, bla-SHV, and bla-TEM were detected in Serratia rubidiae and Enterobacter gergoviae, described as an unusual etiology for newborn sepsis, and docking supported meropenem’s antibacterial effectiveness. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.
Abstract
ABSTRACT High-risk neonatal sepsis resulting in up to four million infants’ mortality and organ damages during the first 28 days of life predominates in low-income countries. We investigated genotypic antibiotic-resistant genes in emerging bacterial pathogens from newborn sepsis cases in Lagos. A cross-sectional study of 294 at-risk hospitalised newborns in prominent paediatrics hospitals throughout three senatorial districts of Lagos utilised the socio-demographic data of their mothers and informed consents. We aseptically collected approximately 3 mL of venous blood from neonates, subsequently isolated and identified the associated bacteria using agar culture on Mueller Hinton agar. To evaluate the antibiotic resistance genes, we used Kirby-Bauer disc diffusion technique; MICROBACT TM Identification System and molecular techniques including PCR, RAPD-PCR, sequencing and docking. From 294 patient samples, 110 (37.4%) were cultured positive, with men (47%), females (53%), ages ≤ 72 hours (71.8%), and ages > 72 hours (28.1%). Klebsiella pneumoniae, K. oxytoca, Enterobacter gergoviae, Serratia rubidiae, Escherichia coli, and Cronobacter sakazakii were the involved Gram- negative bacteria, whereas Coagulase-negative Staphylococci , Staphylococcus aureus , and Enterococcus faecalis were the involved Gram-positive bacteria. Fourteen (12.7%) positive bacteria were resistant to only three antibiotic classes, whereas 73 (66.4%) were resistant to over three antibiotics. Antibiotic- resistant genes bla-CTX, bla-SHV, and bla-TEM were found in Serratia rubidiae and Enterobacter gergoviae , unusual aetiology of newborn sepsis. Molecular docking proved Meropenem’s antibacterial effectiveness. The identified antimicrobial-resistant genes are crucial for improved patient management, as high- resistance strains of ampicillin, gentamicin, and cefotaxime remain ineffective against bacterial sepsis, leaving Meropenem as the sole feasible therapeutic option.
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ABSTRACT
High-risk neonatal sepsis resulting in up to four million infants’ mortality and organ damages during the first 28 days of life predominates in low-income countries. We investigated genotypic antibiotic-resistant genes in emerging bacterial pathogens from newborn sepsis cases in Lagos.
A cross-sectional study of 294 at-risk hospitalised newborns in prominent paediatrics hospitals throughout three senatorial districts of Lagos utilised the socio-demographic data of their mothers and informed consents. We aseptically collected approximately 3 mL of venous blood from neonates, subsequently isolated and identified the associated bacteria using agar culture on Mueller Hinton agar. To evaluate the antibiotic resistance genes, we used Kirby-Bauer disc diffusion technique; MICROBACTTM Identification System and molecular techniques including PCR, RAPD-PCR, sequencing and docking.
From 294 patient samples, 110 (37.4%) were cultured positive, with men (47%), females (53%), ages ≤ 72 hours (71.8%), and ages > 72 hours (28.1%). Klebsiella pneumoniae, K. oxytoca, Enterobacter gergoviae, Serratia rubidiae, Escherichia coli, and Cronobacter sakazakii were the involved Gram- negative bacteria, whereas Coagulase-negative Staphylococci, Staphylococcus aureus, and Enterococcus faecalis were the involved Gram-positive bacteria. Fourteen (12.7%) positive bacteria were resistant to only three antibiotic classes, whereas 73 (66.4%) were resistant to over three antibiotics. Antibiotic- resistant genes bla-CTX, bla-SHV, and bla-TEM were found in Serratia rubidiae and Enterobacter gergoviae, unusual aetiology of newborn sepsis. Molecular docking proved Meropenem’s antibacterial effectiveness.
The identified antimicrobial-resistant genes are crucial for improved patient management, as high- resistance strains of ampicillin, gentamicin, and cefotaxime remain ineffective against bacterial sepsis, leaving Meropenem as the sole feasible therapeutic option.
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