Effects of Differently Incubated Cupric Oxide Nanoparticles on the Granulosa Cells of Caprine Ovary in Vitro

Abstract Present study was aimed to investigate the effect of temperature on the shape and size of nanoparticles and related cytotoxicity of these particles on ovarian granulosa cells. Cupric oxide nanoparticles (CuONPs) were synthesized using a simple, efficient, and reproducible precipitation method involving reduction of Cu metal salt with sodium hydroxide and then incubation of the precipitates at 70oC for 5 hrs. Subsequently, this prepared sample was divided into 3 subsamples and incubated at 3 different temperatures i.e. 70oC, 150oC, and 350oC for a time duration of 5 hrs. to study the effect of temperature on the particles. The products were characterized by XRD, FTIR, HRTEM, and FESEM. Characterization of the particles revealed that all particles were monoclinic crystalline in nature and had a size range from 9 nm - 60 nm. Particles were of different shapes; spherical, needle, and capsule. Toxicity of each particle was determined on granulosa cells by exposing them for 24 hrs. at 2 different doses. Toxicological results showed the size and shape-related toxicity of nanoparticles; particles which were spherical shape were significantly more toxic than capsule-shaped particles.