Effects of microgravity and hypergravity on the longevity of the nematode Caenorhabditis elegans and their association with the DAF-16/FOXO transcription factor

Effects of microgravity and hypergravity on the longevity of the nematode Caenorhabditis elegans and their association with the DAF-16/FOXO transcription factor | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effects of microgravity and hypergravity on the longevity of the nematode Caenorhabditis elegans and their association with the DAF-16/FOXO transcription factor Yoko Honda, Sachiko Yano, Kana Kuriyama, Toko Hashizume, Akira Higashibata, and 14 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7135667/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The effects of altered gravity on longevity remains unknown. We investigated the effects of exposure to space microgravity and hypergravity on the longevity of Caenorhabditis elegans throughout its adult life. Microgravity was demonstrated to shorten lifespan and accelerate the rate of aging compared to artificial 1G conditions in orbit. Conversely, hypergravity extended the lifespan and delayed aging. The lifespan of deletion mutants in the daf-16 gene, which encodes the DAF-16/FOXO transcription factor that regulates stress, sensation, and aging, was shortened by microgravity more than that of the wild type and not extended by hypergravity. We identified several DAF-16 target genes involved in the lifespan extension induced by hypergravity, including cav-1 , hsp-17, acdh-1 , and ZK105.13 , which were upregulated by DAF-16 in hypergravity and tended to be downregulated in microgravity. These findings suggest that gravity influences longevity in C. elegans , at least partly, through the regulation of DAF-16. Molecular Genetics gravity longevity C. elegans lifespan aging DAF-16/FOXO transcription factor Full Text Additional Declarations The authors declare no competing interests. Supplementary Files HONDASupportingInformationFigsTables.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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transcription factor\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":true,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Waseda University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"gravity, longevity, C. elegans, lifespan, aging, DAF-16/FOXO 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We investigated the effects of exposure to space microgravity and hypergravity on the longevity of \u003cem\u003eCaenorhabditis elegans\u003c/em\u003e throughout its adult life. Microgravity was demonstrated to shorten lifespan and accelerate the rate of aging compared to artificial 1G conditions in orbit. Conversely, hypergravity extended the lifespan and delayed aging. The lifespan of deletion mutants in the \u003cem\u003edaf-16\u003c/em\u003e gene, which encodes the DAF-16/FOXO transcription factor that regulates stress, sensation, and aging, was shortened by microgravity more than that of the wild type and not extended by hypergravity. We identified several DAF-16 target genes involved in the lifespan extension induced by hypergravity, including \u003cem\u003ecav-1\u003c/em\u003e, \u003cem\u003ehsp-17, acdh-1\u003c/em\u003e, and \u003cem\u003eZK105.13\u003c/em\u003e, which were upregulated by DAF-16 in hypergravity and tended to be downregulated in microgravity. 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