Annotation of Helicobacter pylori mass peaks detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry

Annotation of Helicobacter pylori mass peaks detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Short Report Annotation of Helicobacter pylori mass peaks detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry Wenfa Ng This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-2106372/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Helicobacter pylori is an important human gastric pathogen that has been studied by the relatively new technique of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This work attempts to annotate the mass peaks of two strains of H. pylori (J99 and ATCC 26695) that has been reported in the literature in 2010 out of pure curiosity. Reference proteome used in this work was H. pylori proteome deposited in UniProt, and the annotation process taken was aided by an in-house MATLAB mass peak annotation software. Briefly, the mass peaks reported were compared to the molecular weight of each protein in the H. pylori proteome to help find a match of lowest error in molecular weight. Results revealed that most of the annotated mass peaks were from ribosomal proteins, especially those in the large ribosome subunit. Other annotated proteins were uncharacterised proteins, which suggest that important facet of this important H. pylori pathogen remains unknown to the biology and biomedical community. Overall, annotation of the mass peaks of two strains of H. pylori should provide a useful resource for other works trying to understand the origins of the mass peaks and their physiological roles in the organism and in human pathogenesis. Applied & Industrial Microbiology General Biochemistry Bioinformatics General Cell Biology & Physiology Analytical Biochemistry Helicobacter pylori MALDI-TOF MS annotation of mass peaks smallest error physiology Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-2106372","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Short Report","associatedPublications":[],"authors":[{"id":139815665,"identity":"bf79720e-dabb-41d7-a38f-6b95c9a3478d","order_by":0,"name":"Wenfa Ng","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA70lEQVRIiWNgGAWjYNACG4b6/YeZj4HZbOxEaUljYGw4zpbGwJAA1MJMtJbzPGZgLQyEtJjzrzF8XJBgx8zYzPPtwccf2+T5mBkYP3zMwa3FcsYbY+MZCclszMy82w1nJNw2bGNmYJacuQ23FoMbZ7dJ8/5g5mFj5t0mzZNwmxGoBcTGq2X7b56EegkeZp5nIC32hLWc793GzJNw2EACaBFISyIRtvB/Bqo8nmDAzGYmOSPtdnIbMCTw++X8scTPPAnVCQb8h59JfLC5bTu/vfngh494tDBIJGAIMTbgUQ8E/Afwy4+CUTAKRsEoYAAABdpKCXP4Qb0AAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0001-7629-2140","institution":"National University of Singapore","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Wenfa","middleName":"","lastName":"Ng","suffix":""}],"badges":[],"createdAt":"2022-09-27 01:11:53","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":true,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false,"coiExplicitlySet":false},"doi":"10.21203/rs.3.rs-2106372/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-2106372/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":27049447,"identity":"5da183b8-7a21-4088-b520-4d3cde08fdd4","added_by":"auto","created_at":"2022-09-27 20:51:46","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":194716,"visible":true,"origin":"","legend":"","description":"","filename":"AnnotationofHelicobacterpylorimasspeaks26Sep2022.pdf","url":"https://assets-eu.researchsquare.com/files/rs-2106372/v1_covered.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eAnnotation of Helicobacter pylori mass peaks detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-2106372/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"National University of Singapore","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Helicobacter pylori, MALDI-TOF MS, annotation of mass peaks, smallest error, physiology,","lastPublishedDoi":"10.21203/rs.3.rs-2106372/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-2106372/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cem\u003eHelicobacter pylori\u003c/em\u003e is an important human gastric pathogen that has been studied by the relatively new technique of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This work attempts to annotate the mass peaks of two strains of \u003cem\u003eH. pylori\u003c/em\u003e (J99 and ATCC 26695) that has been reported in the literature in 2010 out of pure curiosity. Reference proteome used in this work was \u003cem\u003eH. pylori\u003c/em\u003e proteome deposited in UniProt, and the annotation process taken was aided by an in-house MATLAB mass peak annotation software. Briefly, the mass peaks reported were compared to the molecular weight of each protein in the \u003cem\u003eH. pylori\u003c/em\u003e proteome to help find a match of lowest error in molecular weight. Results revealed that most of the annotated mass peaks were from ribosomal proteins, especially those in the large ribosome subunit. Other annotated proteins were uncharacterised proteins, which suggest that important facet of this important \u003cem\u003eH. pylori\u003c/em\u003e pathogen remains unknown to the biology and biomedical community. Overall, annotation of the mass peaks of two strains of \u003cem\u003eH. pylori\u003c/em\u003e should provide a useful resource for other works trying to understand the origins of the mass peaks and their physiological roles in the organism and in human pathogenesis.\u003c/p\u003e","manuscriptTitle":"Annotation of Helicobacter pylori mass peaks detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-09-27 20:51:31","doi":"10.21203/rs.3.rs-2106372/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b0f9d151-19fb-4f7e-a1ce-e1de2492aad1","owner":[],"postedDate":"September 27th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":15818263,"name":"Applied \u0026 Industrial Microbiology"},{"id":15818264,"name":"General Biochemistry"},{"id":15818265,"name":"Bioinformatics"},{"id":15818266,"name":"General Cell Biology \u0026 Physiology"},{"id":15818267,"name":"Analytical Biochemistry"}],"tags":[],"updatedAt":"2022-09-27T20:51:31+00:00","versionOfRecord":[],"versionCreatedAt":"2022-09-27 20:51:31","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-2106372","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-2106372","identity":"rs-2106372","version":["v1"]},"buildId":"-HB7Z8yhvgn0wM9Nzuekk","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}