Bioinformatics analysis and identification of transcriptional regulation of human FOXG1 gene

Forkhead box G1 (FOXG1) is a transcriptional repressor with high expression in the nervous system. Most research focused on the biological function of FOXG1, however, the transcriptional regulation of the FOXG1 gene was rarely studied. Herein, we analyzed and cloned 2.3kb of the 5’-upstream sequences of the FOXG1 gene to better understand the regulation of the FOXG1 gene expression. The CpG islands and putative transcription factor binding sites were analyzed by bioinformatics methods. Then we defined a small region in the upstream 350bp area which was required for the promoter activity by constructing truncated mutants.