Importance of Leifsonia xyli subsp. xyli and thermotherapy for the Brazilian sugarcane industry

Importance of Leifsonia xyli subsp. xyli and thermotherapy for the Brazilian sugarcane industry | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Importance of Leifsonia xyli subsp. xyli and thermotherapy for the Brazilian sugarcane industry Alfredo Seitti Urashima, Nathália Coraini, Otávio Teixeira, Gilberto Liska This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8855239/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 5 You are reading this latest preprint version Abstract Sugarcane sett is the primary inoculum source of ratoon stunt (RSD). RSD-free setts and thermotherapy are measures to control it, but they are not disseminated in Brazil due to little information on the importance of RSD on modern cultivars. Thus, this study aimed to examine the effect of RSD on RB966928, RB867515, and RB92579 and the effectiveness of thermotherapies. Young canes were submitted to four treatments: RSD-free, RSD-positive, 50°C/2h, 52°C/30min. Plots of twenty seedcanes were planted in 5-meter row, 0.5m between plants and 1.6m between rows in 5 replications totaling 60 plots under completely factorial block design. Data on total stool weight, number of non-millable stools, and weight of millable stools were collected from the first to fourth ratoon and submitted to analysis of variance, Scott-Knott, and the regression model with a negative binominal response. Results showed significant yield loss (15 to 37.4%) by RSD of all cultivars and harvest whereas thermotherapy significantly increased weight (8.8 to 65.4%). RSD also caused increased non-millable stools, from the second ratoon on all cultivars/harvests (6.4 to 33 times) compared to RSD-positive counterpart. Benefit of thermotherapies was cultivar dependent since the short combination did not reduce non-millable stools for RB966928, while it did for RB92579. The effect of RSD on weight of millable stools was also cultivar dependent as healthy and HWT stools of RB966928 significantly differed from the diseased counterparts whereas no difference occurred for RB92579. Saccharum control hot water treatment non-millable canes ratoon stunting disease yield loss Full Text Cite Share Download PDF Status: Under Review Version 1 posted Reviewers agreed at journal 20 Feb, 2026 Reviewers invited by journal 18 Feb, 2026 Editor invited by journal 17 Feb, 2026 Editor assigned by journal 17 Feb, 2026 First submitted to journal 11 Feb, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8855239","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":593406452,"identity":"10eb3b48-724b-48ae-b4b3-c6de17aca038","order_by":0,"name":"Alfredo Seitti Urashima","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA40lEQVRIie3QPQrCMBiA4S98kC5K17q0V0hw7WHi0i4KTuIkgtAugmtFD2FvUAnYJfQMFVeHji6CqZ0cjLo55B0SAnnID4DN9o85pAag4Her8DlSM0Fk7Z5ht4p+IKPl18RdIW2aWRhvUlljTywCd1fQy9xAPIm4zapokqmIaSJ5VgmHK9Mx0pXYT+Tk4AHD/q0QoIAOlgYR6FPwnsiYeU7TXkwEnwhrCUmkYF6vvRgK9olwTci6inimxtPjXr8lVyTlJuKXK4TbLAzctMzrq/4xX+HpbCIvFd1EvgY2m81me9MDex5Dz3CJAIoAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0002-1311-0853","institution":"UFSCar","correspondingAuthor":true,"prefix":"","firstName":"Alfredo","middleName":"Seitti","lastName":"Urashima","suffix":""},{"id":593406453,"identity":"1cf04a6f-676b-454e-b638-2e4ce435f704","order_by":1,"name":"Nathália Coraini","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Nathália","middleName":"","lastName":"Coraini","suffix":""},{"id":593406454,"identity":"041c9f46-e466-4fe2-b926-1b417a94cb33","order_by":2,"name":"Otávio Teixeira","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Otávio","middleName":"","lastName":"Teixeira","suffix":""},{"id":593406455,"identity":"dc44827b-1eee-4ae7-bbac-56e1598e8e59","order_by":3,"name":"Gilberto Liska","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Gilberto","middleName":"","lastName":"Liska","suffix":""}],"badges":[],"createdAt":"2026-02-11 19:52:34","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8855239/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8855239/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":103504666,"identity":"9005af8b-263d-48fe-a770-ec4281dd2e1a","added_by":"auto","created_at":"2026-02-26 13:21:00","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1195508,"visible":true,"origin":"","legend":"","description":"","filename":"Urashimaetal2026Feb11.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8855239/v1_covered_c279a1c8-33f2-4696-93fe-85b1cae99fca.pdf"}],"financialInterests":"","formattedTitle":"Importance of Leifsonia xyli subsp. xyli and thermotherapy for the Brazilian sugarcane industry","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"tropical-plant-pathology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"tppa","sideBox":"Learn more about [Tropical Plant Pathology](https://www.springer.com/journal/40858)","snPcode":"40858","submissionUrl":"https://www.editorialmanager.com/tppa","title":"Tropical Plant Pathology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Saccharum, control, hot water treatment, non-millable canes, ratoon stunting disease, yield loss","lastPublishedDoi":"10.21203/rs.3.rs-8855239/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8855239/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSugarcane sett is the primary inoculum source of ratoon stunt (RSD). RSD-free setts and thermotherapy are measures to control it, but they are not disseminated in Brazil due to little information on the importance of RSD on modern cultivars. Thus, this study aimed to examine the effect of RSD on RB966928, RB867515, and RB92579 and the effectiveness of thermotherapies. Young canes were submitted to four treatments: RSD-free, RSD-positive, 50\u0026deg;C/2h, 52\u0026deg;C/30min. Plots of twenty seedcanes were planted in 5-meter row, 0.5m between plants and 1.6m between rows in 5 replications totaling 60 plots under completely factorial block design. Data on total stool weight, number of non-millable stools, and weight of millable stools were collected from the first to fourth ratoon and submitted to analysis of variance, Scott-Knott, and the regression model with a negative binominal response. Results showed significant yield loss (15 to 37.4%) by RSD of all cultivars and harvest whereas thermotherapy significantly increased weight (8.8 to 65.4%). 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