Simultaneous determination of C4-C9 alkylphenols and bisphenol A in environmental water at the Yellow River Estuary Area in China by gas chromatography-mass spectrometry

Simultaneous determination of C4-C9 alkylphenols and bisphenol A in environmental water at the Yellow River Estuary Area in China by gas chromatography-mass spectrometry | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Simultaneous determination of C4-C9 alkylphenols and bisphenol A in environmental water at the Yellow River Estuary Area in China by gas chromatography-mass spectrometry hailliang yin, Tongna ZHOU, ziqi guo, xinliang liu, jian ZHAO This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-195989/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A liquid-liquid extraction combined with derivatization and gas chromatography-mass spectrometry (GC-MS) method was developed for the rapid determination of C4-C9 alkylphenols (4-tert-butylphenol, 4-n-butylphenol, 4-n-pentylphenol, 4-n-hexylphenol, 4-n-heptylphenol, 4-n-octylphenol, 4-tert-octylphenol, 4-n-nonylphenol and 4-nonylphenol) and bisphenol A in the environmental water. The extraction solvent, extraction time, solvent volume, acidity, salt content and the distillation degree were optimized. After drying and dehydration, the extract was derivatized, detected by GC-MS and quantified by internal standard method. After the dehydration, the extract was derivatized with BSTFA, detected by GC-MS and quantified by internal standard method. The results showed that the relative standard deviations of the relative response factors of the target compounds were less than 20%, the method detection limits were 0.002 µg/Lཞ0.006 µg/L, the relative standard deviations of the three spiked levels were 0.67%ཞ13.7%, and the average recoveries of the actual water samples were 68.0%ཞ122%. The method precision and accuracy were good. The method is simple, rapid, accurate, stable and reliable, which is suitable for the detection of target in water. The contents of C4-C9 alkylphenols and bisphenol A in groundwater, surface water, sewage, waste water and sea water samples in Dongying City located at the Yellow River Estuary were determined by this method. The results showed that the contents of 4-nonylphenol and bisphenol A in these samples were lower than the home and abroad requirements. Environmental Engineering Environmental water C4-C9 alkylphenol Bisphenol A Gas chromatography-mass spectrometry Liquid-liquid extraction Yellow River Estuary area Figures Figure 1 Introduction As the typical environmental endocrine disruptors, alkylphenols and bisphenol A have been the focus of attention (Wen et al. 2020; Abu-Alsoud and Bottaro 2021; Liu and Zhao 2020; Murray et al. 2017). There is no domestic standard method to monitor the alkylphenols and bisphenol A in water in China. ASTM, ISO and the Japanese government have developed the corresponding monitoring standard methods (ISO 18857-2 2009; ASTM D7065 2011; ASTM D7574 2009; JIS K 0450-10-10 2006). However, these above monitoring methods were developed early and the research objects are single, which are limited to one or several compounds of 4-tert-octylphenol, nonylphenol and bisphenol A. This is related to the early people's understanding of the use of alkylphenols. The plastic industry developed early, and the nonylphenol and octylphenol were the most widely used in the plastic industry. As a result, the proportion of nonylphenol and octylphenol in the environmental water was high, and the other alkylphenols were not detected or the detection value was very low, which did not attract people's attention. There are many detection methods of alkylphenols and bisphenol A in water, among which the liquid-liquid extraction combined GC-MS method is a more studied method, but the research objects are also concentrated on one or several compounds of 4-tert-octylphenol, nonylphenol and bisphenol A (Selvaraj et al. 2014; Shen et al. 2015; Santhi et al. 2012; Dong et al. 2015; Wang et al. 2013; Martinez and Peñuela 2013). However, with the development of new plastics and liquid crystal products, many new C4-C9 alkylphenols have been widely used. The 4-tert-butylphenol is an important raw material for the production of p-tert-butylphenol formaldehyde resin. The 4-n-pentylphenol is an effective component of disinfectant, food preservative and deodorant. The 4-n-butylphenol, 4-n-hexylphenol and 4-n-heptylphenol are often used as liquid crystal raw materials and intermediates. The wide application of C4-C9 alkylphenols leads to the continuous entry of these new alkylphenols into the environmental water. These C4-C9 alkylphenols are also the endocrine disruptors, which can also affect the performance of human endocrine system. In 1998, the Ministry of environment of Japan listed 4-pentyl phenol and other alkyl phenols as the endocrine disruptors (Ministry of the Environment of Japan 1998). However, there is no standard method for the monitoring of these C4-C9 alkylphenol and bisphenol A in water in China, and there is no standard method developed by the authoritative institutions abroad. The alkylphenol is a combination of phenol and alkyl chain. Due to the different length of alkyl chain, the physical and chemical properties of these C4-C9 alkylphenols are different, such as nonpolarity, adsorption capacity on the material surface and partition coefficient between aqueous phase and organic phase. The greater the difference of alkyl chain length, the greater the difference of these properties. The quantitative determination of these C4-C9 alkylphenols and bisphenol A in groundwater, surface water, sewage and wastewater, seawater and other actual water samples is an analytical technique for trace or ultra trace mixed components in complex matrix, which is difficult to extract, enrich, separate and detect. Liquid-liquid extraction has the advantages of good separation effect, simple operation and high repeatability, which is the most widely used organic matter extraction and enrichment technology (Wang et al. 2020; Deng et al. 2018). GC-MS is the most advanced and reliable detection technology (Subuhi et al. 2020; Azzouz et al. 2020). After the derivatization, the polarity and boiling point of these alkylphenols and bisphenol A decreased, the volatility and stability increased, the anti-interference ability increased, and the samples could be stored for a long time for retest (Liu et al. 2019; Tan et al. 2019). In this study, a liquid-liquid extraction combined with derivatization and GC-MS method with low detection limit, high recovery, good reproducibility and rapid analysis was established. Nine C4-C9 alkylphenols (4-tert-butyl phenol, 4-n-butyl phenol, 4-n-pentyl phenol, 4-hexyl phenol, 4-heptyl phenol, 4-octyl phenol, 4-tert-octyl phenol, 4-nonylphenol and 4-nonylphenol) and bisphenol A in water were analyzed. The accurate concentrations of target substances in groundwater, surface water, sewage, waste water and sea water were determined. Materials And Methods 1.1 Materials and reagents The standard solutions of C4-C9 alkylphenols and bisphenol A (including 4-tert-butyl phenol, 4-n-butyl phenol, 4-n-pentyl phenol, 4-n-hexyl phenol, 4-n-heptyl phenol, 4-n-octyl phenol, 4-tert-octyl phenol, 4-n-nonylphenol, 4-nonylphenol and bisphenol A, with the concentration of 1000 μg/ml), deuterated bisphenol A-d 16 standard solution (1000 μg/ml), deuterated naphthalene-d 8 standard solution (200 μg/ml), deuterated phenanthrene-d 10 standard solution (500 μg/ml), and deuterated pyrene-d 10 standard solution (500 μg/ml), were purchased from Drehrenstorfer, Germany. Dichloromethane, acetone and n-hexane are pesticide residues, purchased from Merda company of USA. The derivatization reagent BSTFA (containing 1% TMCs) was purchased from Drehrenstorfer, Germany. Hydrochloric acid, sodium chloride and anhydrous sodium sulfate are of high grade purity purchased from Sinopharm group of China. Sodium chloride and anhydrous sodium sulfate are roasted at 450 ℃ . Alkylphenols and bisphenol A working solution (1 μg/ml), substitute solution (1 μg/ml of deuterated bisphenol A-d 16 ), and internal standard (1 μg/ml of deuterated naphthalene-d 8 , deuterated phenanthrene-d 10 , and deuterated pyrene-d 10 , respectively) were diluted from the corresponding standard solutions with acetone. 1.2 Instruments and equipment Agilent 7890B gas chromatograph, Agilent 5977B mass spectrometer, Heidolph rotary evaporator. 1.3 Solution preparation and standard curve Water treatment steps: Measured 500 ml water sample into the separating funnel, added 100 µl substitute working solution and mixed well. Then added 10 g sodium chloride, shaked to complete dissolution, added 30 ml dichloromethane, shaked violently for 10 min, standed for 5-10 min, separated layers, collected organic phase, extracted again for 1-2 times, combined organic phase and dehydrated with anhydrous sodium sulfate, and concentrated organic phase to 0.5 ml. Transfered the concentrated sample to a 1 ml volumetric flask, washed the concentrated flask with a small amount of dichloromethane, and combined the washing solution into the volumetric flask. Added 100 µl of internal standard working solution and 100 µl of derivatization reagent in turn, and diluted to 1 ml with dichloromethane. The samples were obtained by derivatization at room temperature for 1 h. Standard curve preparation: Added 100 μl internal standard working solution and 100 μl derivatization reagent respectively, and used dichloromethane to make volume to 1 ml. The low concentration calibration series are 0 μg/L, 5.0 μg/L, 10.0 μg/L, 20.0 μg/L, 40.0 μg/L, 60.0 μg/L and 80.0 μg/L, while the high concentration calibration series are 60.0 μg/L, 100 μg/L, 200 μg/L, 500 μg/L, 1000 μg/L and 2000 μg/L. After derivatization at room temperature for 1 h, the target compounds were determined from low concentration to high concentration according to the chromatographic reference conditions. The retention time and quantitative ion response values of the standard series of target compounds and corresponding internal standards were recorded. The average relative response factor was used to draw the calibration curve, and the relative standard deviation (RSD) of the relative response factor (RRF) of the standard series of target compounds should not be more than 20%. 1.4 Actual water sample collection According to the relevant environmental protection standards (HJ 91.1 2019; HJ/T 164 2020; HJ/T 493 2009), the sewage, river water, the drainage of wastewater treatment plant and Bohai Sea water were collected in Dongying City. The hydrochloric acid solution was added to adjust the pH of the water sample to be less than or equal to 2. The water sample should be filled with the sample bottle and sealed. It should be stored under 4 ℃ in dark and refrigerated. The extraction and derivation should be completed within 10 days, and the analysis should be completed within 5 days after the extraction. 1.5 Establishment of analytical method (1) Gas chromatographic conditions Injection port temperature: 300 ℃, no split injection. He gas pressure: 40 kPa for 5 min, 2 kPa/min to 70 kPa, 5 min; column temperature: 50 ℃, 2 min, 20 ℃/min to 100 ℃, 10 ℃/min to 200 ℃, 20 ℃/min to 300 ℃, 5 min; injection volume: 1 μl. The chromatographic column was hp-5ms capillary column, 30 m long, 0.25 mm inner diameter and 0.25 μm film thickness. (2) Mass spectrometry conditions Transmission line temperature: 280 ℃. Quadrupole temperature: 150 ℃. Ion source temperature: 230 ℃. Electron energy of ion source: 70 ev. Mass range: 35-400 amu. Data acquisition mode: SIM. The quantitative and qualitative ions are shown in Table 1. Table 1 Quantitative ion and qualitative ion of target compounds Compound Name CAS number Quantitative ion Qualitative ion Note deuterated naphthalene-d 8 （IS 1） 1146-65-2 136 — — 4-tert-butyl phenol 98-54-4 207 222 IS 1 4-n-butyl phenol 1638-22-8 179 222 IS 1 4-n-pentyl phenol 14938-35-3 179 236 IS 1 4-n-hexyl phenol 2446-69-7 179 250 IS 1 4-tert-octyl phenol 140-66-9 207 278 IS 1 4-n-heptyl phenol 1987-50-4 179 264 IS 2 4-nonylphenol 84852-15-3 207 221、193 IS 2 deuterated phenanthrene-d 10 （IS 2） 1517-22-2 188 — — 4-n-octyl phenol 1806-26-4 179 278 IS 2 4-n-nonylphenol 104-40-5 179 292 IS 2 deuterated pyrene-d 10 （IS 3） 1718-52-1 212 — — deuterated bisphenol A-d 16 （Surrogate） 96210-87-6 368 386 IS 3 bisphenol A 80-05-7 357 372 IS 3 Results And Discussion 2.1 Influence of extraction solvent 500 ml of laboratory water was added into the separating funnel, and then alkylphenol and bisphenol A working solution were added to make the concentration of target substance in the water sample 0.20 µg/L. The effects of different extraction solvents on the recovery of target compounds are shown in Table 2. According to the data in the table, dichloromethane has the best extraction effect. Table 2 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction solvents Extraction solvent Recovery rate % 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Dichloromethane 104 101 115 101 98.7 110 95.4 109 120 98.8 Hexane 118 13.5 83. 94.1 108 97.6 96.5 107 87.5 106 Ethyl acetate 91.2 84.2 83.7 65.8 72.1 80.6 65.4 82.9 66.5 76.2 Toluene 65.3 88.9 65.0 78.5 72.0 83.6 79.4 88.1 75.4 55.1 2.2 Influence of extraction times The effects of different extraction times of dichloromethane on the recovery of target compounds are shown in Table 3. It can be seen from the table that C4-C9 alkylphenols can be extracted completely after one extraction, and bisphenol A can be extracted mainly after two extraction. When dichloromethane is selected as the extraction solvent, the second extraction can meet the requirements. Table 3 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction times Extraction times Recovery rate % 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Surrogate 1 97.8 102 113 109 119 117 96.9 117 103 76.3 62.6 2 1.9 2.0 n.d. n.d. n.d. n.d. n.d. n.d. 1.0 24.9 27.4 3 n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. 4.6 5.2 4 n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. 2.3 Influence of extraction time The influence of different extraction time on the recovery rate of target compounds is shown in Table 4. It can be seen that the extraction time has a slight effect on the extraction efficiency. With the extension of the extraction time, the recovery rate of the target substance increases slightly. It can be seen from the table that the extraction time of 5 minutes is enough to fully recover the target substance in the water sample. Table 4 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction time Extraction time（min） Recovery rate % 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Surrogate 5 98.9 101 109 101 118 112 95.6 115 106 98.7 91.0 10 99.7 104 113 109 119 117 96.9 117 104 101 90.0 15 104 109 118 117 122 127 98.1 123 106 101 92.3 2.4 Influence of acidity in water The effect of acidity in water on the recovery rate of target compounds is shown in Table 5. It can be seen from the table that when the pH value is higher than 6, the recoveries of 4-nonylphenol and 4-n-octyl phenol decrease, but the recoveries of other target compounds are not affected. Considering that acidic conditions are conducive to inhibiting bacteria in water and preventing bacteria from consuming alkylphenol organics, the pH value of water sample is usually less than 2. Table 5 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different pH value pH 值 <1 1-2 2-3 3-4 4-5 6-7 4-tert-butyl phenol 107 98.6 107 102 99.0 100 4-n-butyl phenol 110 99 104 101 99.4 96.8 4-n-pentyl phenol 117 111 113 110 109 106 4-n-hexyl phenol 117 109 112 108 106 102 4-tert-octyl phenol 122 106 115 110 109 110 4-n-heptyl phenol 125 115 120 114 113 105 4-nonylphenol 105 97.3 105 101 97.4 91.3 4-n-octyl phenol 116 106 112 107 107 97.7 4-n-nonylphenol 121 110 121 115 120 108 bisphenol A 110 115 116 108 114 113 Surrogate 117 110 121 115 114 113 2.5 Influence of extraction solvent volume The effects of different extraction solvent volumes on the recovery of target compounds are shown in Table 6. It can be seen from the table that 20 ml of dichloromethane can meet the extraction requirements. Table 6 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction solvent volume Extraction solvent volume（ml） Recovery rate % 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Surrogate 20 95.2 99.7 111 97.0 104 116 95.2 96.7 99.0 98.4 100 30 104 109 116 100 104 118 95.2 96.3 97.6 101 103 40 107 111 118 101 103 117 98.3 97.9 97.1 103 104 50 108 113 121 104 106 122 100 101 103 104 104 2.6 Influence of salt content Phenols are water-soluble and need salting out to improve extraction efficiency. The effect of different salt amount on the recovery rate of target substance is shown in Table 7. It can be seen from the table that adding sodium chloride can indeed improve the recovery rate of the target substance, but the improvement effect is not significant. The main reason is that the recovery rate data is high enough without adding salt. After comprehensive consideration, 5g sodium chloride can be added in water sample extraction. Table 7 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different salt content NaCl Dosage (g) 0 5 10 20 30 4-tert-butyl phenol 97.5 100 103 103 102 4-n-butyl phenol 97.3 102 103 106 107 4-n-pentyl phenol 107 110 112 115 114 4-n-hexyl phenol 100 106 108 111 112 4-tert-octyl phenol 107 110 110 114 110 4-n-heptyl phenol 102 110 112 115 115 4-nonylphenol 93.9 104 103 105 105 4-n-octyl phenol 95.6 103 109 107 108 4-n-nonylphenol 99.1 107 113 112 111 bisphenol A 107 108 115 110 114 Surrogate 97.8 101 106 109 108 2.7 Effect of drying time during concentration process In the standard of organic matter determination in water, when liquid-liquid extraction is used, it is related to whether the extraction liquid is dried during the concentration process. For C4-C9 alkylphenols, some of the target compounds are liquid with vapor pressure, and volatilization occurs when the vacuum is appropriate. Some of the solid targets are powder or velvet with low density, and they also have drift loss when the vacuum is appropriate. The influence of organic phase concentration to 0.5 ml or concentration to dryness and continuous rotary evaporation for different time on the recovery of target substance is shown in Table 8. It can be seen from the table that drying can significantly reduce the detection results, especially for some alkylphenols (4-tert-butylphenyl, etc.) which are liquid at room temperature. The experimental results are consistent with the above analysis. Finally, in the process of concentration, the treatment mode without drying was selected. Table 8 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different drying process Drying time（min） Condense to 0.5 ml 1 5 10 4-tert-butyl phenol 102 40.1 21.5 20.9 4-n-butyl phenol 100 54.0 22.3 18.8 4-n-pentyl phenol 104 73.2 32.3 23.2 4-n-hexyl phenol 102 84.6 56.1 44.4 4-tert-octyl phenol 103 88.1 60.7 53.7 4-n-heptyl phenol 104 90.9 79.4 74.4 4-nonylphenol 100 92.3 82.5 74.9 4-n-octyl phenol 99.6 90.5 87.8 84.7 4-n-nonylphenol 104 95.4 94.1 92.6 bisphenol A 102 93.6 94.6 92.1 Surrogate 101 95.1 96.0 92.9 2.8 Spectrogram The chromatogram of the standard sample of the target analytes is shown in Fig 1. It can be seen from the figure that under the conditions of reference gas chromatography-mass spectrometry, the peaks of each compound are independent and clear. Due to the use of derivatization treatment, there is basically no tailing phenomenon of phenolic compounds. 4-nonylphenol is a mixed peak of one group of isomers, and other compounds are single peaks. 2.9 Standard curve The linear correlation coefficient of the standard curve of the object to be measured is shown in Table 9. It can be seen from the table that the standard curves of the target compounds are linear. Table 9 Linear correlation coefficient, detection limit and lower limit of determination of C4-C9 alkylphenols and bisphenol A RSD of RRF 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Low concentration curve 12.2% 6.02% 6.21% 6.30% 5.85% 6.71% 8.00% 4.04% 4.31% 3.05% High concentration curve 1.71% 3.42% 4.87% 7.03% 5.36% 10.7% 3.18% 9.64% 9.64% 6.47% detection limit(μg/L) 0.002 0.002 0.002 0.002 0.002 0.002 0.006 0.002 0.003 0.003 Lower limit of determination(μg/L) 0.008 0.008 0.008 0.008 0.008 0.008 0.024 0.008 0.012 0.012 2.10 Method detection limit The detection limit of the method was confirmed according to the relevant environmental protection standard method (HJ 168 2020), and seven laboratory blank samples with or without the target objects working solution were analyzed continuously. Because 4-tert-butylphenyl, 4-tert-octylphenyl, 4-nonylphenol and bisphenol A were detected in the laboratory blank, the detection limits of these target substances were determined by the method of laboratory blank without standard addition and the detection limits of the remaining analytes were determined by the method of laboratory blank with standard addition. The concentration of these remaining analytes in the laboratory blank sample with standard addition is all 0.010 μg/L. The lower limit of determination is 4 times of the detection limit and both of which are listed in Table 9. These detection limits of the target compounds changes from 0.002 μg/L to 0.006 μg/L and the lower limit of determination from 0.008 μg/L to 0.024 μg/L. 2.11 Recovery and precision Taking the laboratory water without the target analytes as the blank matrix, C4-C9 alkylphenols and bisphenol A standard solutions with three different concentration levels were added respectively. The pretreatment and test were carried out according to the method steps. Each concentration level was determined for six times in parallel. The relative standard deviation results are shown in table 10. Take surface water, sewage and wastewater as sample matrix, add C4-C9 alkylphenols and bisphenol A standard solution with matrix concentration level of 0.5-3 times, repeat the above laboratory test, and the average recovery results are shown in Table 11. The relative standard deviations of the target samples ranged from 0.67% to 13.7%, and the average recovery ranged from 68.0% to 122%, which indicates that the precision and accuracy of the experiment are good. Table 10 Precision datas Spiked concentration (µg/L) Relative standard deviation% 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A 0.02 9.52 4.62 3.93 2.90 2.68 4.69 10.1 10.2 12.3 7.08 0.50 3.20 2.15 1.88 1.92 1.96 1.79 2.49 2.29 3.12 2.44 2.0 2.05 2.42 2.12 2.19 1.51 1.74 5.32 7.20 13.7 0.67 Table 11 Recovery datas Water sample Spiked concentration(µg/L) Recovery rate of standard addition % 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Sewage 0.05 106 96.0 111 104 102 101 90.7 112 99.1 107 Surface water 0.50 99.5 102 110 116 121 122 72.4 79.1 88.2 68.0 Wastewater 2.0 95.9 102 104 107 104 111 97.9 102 98.6 91.4 2.12 Determination of typical representative water samples The contents of target analytes in groundwater, surface water, sewage, wastewater and seawater samples are shown in Table 12. It can be seen from the table that the contents of 4-nonylphenol and bisphenol A in the environmental water in Dongying City are below the requirements of relevant directives at home and abroad (GB 31572 2015; GB 31571 2015; U.S. EPA 2005; European Commission EC 2003), and the contents of target substances in groundwater, surface water and seawater are very low. Table 12 The measured value of target analytes in actual water sample（µg/L） 4-tert-butyl phenol 4-n-butyl phenol 4-n-pentyl phenol 4-n-hexyl phenol 4-tert-octyl phenol 4-n-heptyl phenol 4-nonylphenol 4-n-octyl phenol 4-n-nonylphenol bisphenol A Surrogate Recovery Groundwater n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. 0.016 124% Surface water 1 n.d. n.d. n.d. n.d. 0.003 n.d. 0.049 n.d. n.d. 0.023 125% Surface water 2 n.d. n.d. n.d. n.d. n.d. n.d. 0.026 n.d. n.d. 0.032 78.6% Surface water 3 0.031 0.007 n.d. n.d. 0.008 0.002 0.139 0.002 0.003 0.068 92.3% Surface water 4 n.d. n.d. n.d. n.d. n.d. n.d. 0.228 n.d. n.d. 0.226 77.4% Sewage 1 0.004 0.008 n.d. n.d. 0.019 0.003 0.965 0.003 n.d. 0.024 110% Sewage 2 n.d. n.d. n.d. n.d. n.d. n.d. 0.344 n.d. n.d. 0.156 88.5% Wastewater 1 0.002 n.d. n.d. n.d. 0.007 n.d. 1.37 n.d. 0.001 0.200 115% Wastewater 2 n.d. n.d. n.d. n.d. n.d. n.d. 0.207 n.d. n.d. 0.029 81.9% Wastewater 3 n.d. n.d. n.d. n.d. n.d. n.d. 1.81 n.d. n.d. 2.30 70.5% Wastewater 4 0.006 0.004 0.005 0.007 0.016 0.017 0.218 0.064 0.094 0.870 97.9% Wastewater 5 0.004 n.d. n.d. n.d. 0.009 n.d. 0.114 0.026 0.035 32.8 87.3% Seawater n.d. 0.003 0.001 n.d. n.d. 0.002 0.077 0.008 n.d. 0.011 104% Note: surface water 1 is drinking water, surface water 2 is yellow river water, surface water 3 is Guangli River water, surface water 4 is Xiaoqing River water, sewage 1 is outlet water of a sewage treatment plant in Dongying City, sewage 2 is outlet water of a sewage treatment plant in Guangrao County, Dongying City, wastewater 1 is outlet water of an industrial wastewater treatment plant in Dongying City, and wastewater 2 is outlet water of an oil processing enterprise in Dongying City, wastewater 3 is from the workshop of an oil processing enterprise in Dongying City, wastewater 4 is from the workshop of a bisphenol A production enterprise in Dongying City, and wastewater 5 is from the workshop of a bisphenol A production enterprise in Dongying City. Conclusions (1) Aiming to monitor of C4-C9 alkylphenols and bisphenol A in water, A liquid-liquid extraction combined with derivatization and gas chromatography-mass spectrometry (GC-MS) method was used to optimize the extraction solvent, extraction times, extraction time, extraction solvent volume, acidity, salt content, rotary distillation concentration degree and other conditions. After optimization, dichloromethane was used as the extraction solvent, the extraction time was 5 min, the extraction solvent was 30 ml, the pH value was less than 2, and the amount of salt was 5 g. (2) The method has the advantages of high sensitivity, low detection limit, accurate quantification and good precision, which can meet the needs of the determination of C4-C9 alkylphenols and bisphenol A in water. (3) The method was used to detect the groundwater, surface water, sewage, wastewater and sea water samples in Dongying City located at the Yellow River Estuary. The results showed that the contents of 4-nonylphenol and bisphenol A in the samples were lower than the requirements of relevant directives at home and abroad. Declarations Acknowledgments We appreciate the help of the Ecological and Environmental Monitoring Center of Dongying Municipal of Shandong Province in sample testing. Authors’ contributions Ziqi Guo, Xinliang Liu and Jian Zhao carried out the experiment. Hailiang Yin and Tongna Zhou put forward the idea. All authors have read and approved the final manuscript. Funding information This work was supported by Standard Development and Revision Project of Ministry of Ecological Environment of China (Grant Nos. 2014-31) , Shandong Provincial Natural Science Foundation, Chian（ZR2019MB022）and the Fundamental Research Funds for the Central Universities (19CX02063A). Availability of data and materials Not applicable. Compliance with ethical standards Ethics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests. References Abu-Alsoud GF, Bottaro CS (2021) Porous thin-film molecularly imprinted polymer device for simultaneous determination of phenol, alkylphenol and chlorophenol compounds in water. Talanta 223:121727 ASTM D7065 (2011) Standard Test Method for Determination of Nonylphenol, Bisphenol A, p-tert- Octylphenol, Nonylphenol Monoethoxylate and. Nonylphenol Diethoxylate in Environmental Waters by Gas Chromatography Mass Spectrometry. ASTM D7574 (2009) Standard Test Method for Determination of Bisphenol A in Environmental Waters by Liquid Chromatography/Tandem Mass Spectrometry. Azzouz A, Colón LP, Hejji L, Ballesteros E (2020) Determination of alkylphenols, phenylphenols, bisphenol A, parabens, organophosphorus pesticides and triclosan in different cereal-based foodstuffs by gas chromatography-mass spectrometry. Anal Bioanal Chem 412:2621-2631 Deng HL, Su XJ, Wang HB (2018) Simultaneous Determination of Aflatoxin B1, Bisphenol A, and 4-Nonylphenol in Peanut Oils by Liquid-Liquid Extraction Combined with Solid-Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. Food Anal Method 11:1303-1311 Dong CD, Chen CW, Chen CF (2015) Seasonal and spatial distribution of 4-nonylphenol and 4-tert-octylphenol in the sediment of Kaohsiung Harbor, Taiwan. Chemosphere 134:588-597 European Commission EC (2003) Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy, Official journal of the european communities, OJ L 327, 22.12.2000, pp 1-73 GB 31572 (2015) Emission standard of pollutants for synthetic resin industry. Beijing. GB 31571 (2015) Emission standard of pollutants for petroleum chemistry industry. Beijing. HJ 168 (2020) Technical guideline for the development of environmental monitoring analytical method standards. Beijing. HJ 91.1 (2019) Technical specifications for wastewater monitoring. Beijing. HJ/T 164 (2020) Technical specifications for environmental monitoring of groundwater. Beijing. HJ/T 493 (2009) Water quality sampling - technical regulation of the preservation and handling of samples. Beijing. ISO 18857-2 (2009) Water quality-Determination of selected alkylphenols-Part 2: Gas chromatographic-mass spectrometric determination of alkylphenols, their ethoxylates and bisphenol A in non-filtered samples following solid-phase extraction and derivatisation. JIS K 0450-10-10 (2006) Testing method for bisphenol A in industrial water and waste water. Liu LY, Zhao Q (2020) A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer . J Environ Sci 32(11):19-24 Liu ZR, Li YY, Sun LW, Yang H, Zheng X, Wang LB (2019) Investigation of diazo‐derivatization of bisphenol A and its applicability for quantitation in food safety inspections using high‐performance liquid chromatography. Biomedical Chromatography 33:e4419 Martinez M, Peñuela GA (2013) Analysis of triclosan and 4-n‐nonylphenol in Colombian reservoir water by gas chromatography‐mass spectrometry. Water Environ J 27:387-395 Ministry of the Environment of Japan (1998) Environment Agency's Basic Policy on Environmental Endocrine Disruptors, Strategic Programs on Environmental Endocrine Disruptors SPEED'98. Tokyo. Murray A, Örmeci B, Lai EPC (2017) Use of sub-micron sized resin particles for removal of endocrine disrupting compounds and pharmaceuticals from water and wastewater . J Environ Sci 29(1):256-264. Santhi VA, Hairin T, Mustafa AM (2012) Simultaneous determination of organochlorine pesticides and bisphenol A in edible marine biota by GC-MS. Chemosphere 86(10):1066-1071 Selvaraj KK, Shanmugam G, Sampath S, Larsson DGJ, Ramaswamy BR (2014) GC-MS determination of bisphenol A and alkylphenol ethoxylates in river water from India and their ecotoxicological risk assessment. Ecotox Environ Safe 99:13-20 Shen G, Yu G, Cai ZX, Zhang ZL (2005) Development of an analytical method to determine phenolic endocrine disrupting chemicals in sewage and sludge by GC/MS. Chinese Sci Bull 50:2681-2687 Subuhi NEAM, Saad SM, Zain NNM, Lim V, Miskam M, Kamaruzaman S, Raoov M, Yahaya N (2020) An efficient biosorption-based dispersive liquid-liquid microextraction with extractant removal by magnetic nanoparticles for quantification of bisphenol A in water samples by gas chromatography-mass spectrometry detection. J Sep Sci 43(16):3294-3303 U.S. EPA (2005) Aquatic Life Ambient Water Quality Criteria Nonylphenol FINAL, Washington DC, EPA-822-R-05-005. Wang DX, Wang XC, Hu QJ, Zhang CX, Li F (2020) Salting-Out Assisted Liquid-Liquid Extraction Coupled to Dispersive Liquid-Liquid Microextraction for the Determination of Bisphenol A and Six Analogs (B, E, F, S, BADGE, BFDGE) in Canned Coffee Drinks by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. Food Anal Method https://doi.org/10.1007/S12161-020-01879-0 Wang SY, Liu F, Liu YL, Chen L (2013) Determination of 12 Isomers of p-Nonylphenol in Groundwater by Gas Chromatography-Mass Spectrometry. Chinese J Anal Chem 43(11):1699-1703 Wen HJ, Chang TC, Ding WH, Tsai SF, Hsiung CA, Wang S L (2020) Exposure to endocrine disruptor alkylphenols and the occurrence of endometrial cancer. Environ Pollut 267:115475 Tan DQ, Jin J, Wang LX, He XL, Guo CC, Lu XB, Chen JP (2019) Quantification of bisphenol A and its selected analogs in serum using pre‐column derivatization with high‐performance liquid chromatography and tandem mass spectrometry. J Sep Sci 42(5):991-998 Supplementary Files GraphicalAbstract.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-195989","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":17783217,"identity":"2cb2b3be-d418-4e0e-b948-cefeb4c01e09","order_by":0,"name":"hailliang yin","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAsUlEQVRIiWNgGAWjYDACZgY2hgcVELYE8VoSzpCkhQGoJbGNFC0Gx3mPPUicdziPv4H54G0eBrs8wloO86UbJG47XCxxgC3ZmochuZgILTxmEkAtiRsYeMykeRgOJDYQp2UOSAv/N1K0NIBtYSNOiyRIS8Kx9GKJw2zGlnMMkglr4Tt/xkziQ411Hn9788MbbyrsCGtROAChE4BxCnInIfVAIN8A0zIKRsEoGAWjABcAALpdNz6ToD/YAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-8049-7138","institution":"China University of Petroleum Huadong","correspondingAuthor":true,"prefix":"","firstName":"hailliang","middleName":"","lastName":"yin","suffix":""},{"id":17783218,"identity":"a811a2d0-a4e0-4a25-a2ce-e40e4e9175cc","order_by":1,"name":"Tongna ZHOU","email":"","orcid":"","institution":"Ecological and environmental monitoring center of dongying of shandong province","correspondingAuthor":false,"prefix":"","firstName":"Tongna","middleName":"","lastName":"ZHOU","suffix":""},{"id":17783219,"identity":"344b67e2-22f4-416c-bb93-e808bb59543b","order_by":2,"name":"ziqi guo","email":"","orcid":"","institution":"China University of Petroleum Huadong","correspondingAuthor":false,"prefix":"","firstName":"ziqi","middleName":"","lastName":"guo","suffix":""},{"id":17783220,"identity":"02117efb-5aa1-4045-9e10-997e6202086d","order_by":3,"name":"xinliang liu","email":"","orcid":"","institution":"China University of Petroleum Huadong","correspondingAuthor":false,"prefix":"","firstName":"xinliang","middleName":"","lastName":"liu","suffix":""},{"id":17783221,"identity":"a392a005-761f-4547-9663-19928ad27cde","order_by":4,"name":"jian ZHAO","email":"","orcid":"","institution":"China University of Petroleum Huadong","correspondingAuthor":false,"prefix":"","firstName":"jian","middleName":"","lastName":"ZHAO","suffix":""}],"badges":[],"createdAt":"2021-02-01 02:51:24","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-195989/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-195989/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":7234654,"identity":"80d5c2cd-a40c-47d0-99e3-61ef1c6d4734","added_by":"auto","created_at":"2021-03-22 19:00:43","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":27870,"visible":true,"origin":"","legend":"Total ion flow diagram of C4-C9 alkylphenols and bisphenol A (SIM)\n1-IS1，2-4-tert-butyl phenol，3-4-n-butyl phenol，4-4-n-pentyl phenol，5-4-n-hexyl phenol，6-4-tert-octyl phenol，7-4-n-heptyl phenol，8-4-nonylphenol，9-IS2，10-4-n-octyl phenol，11-4-n-nonylphenol，12-IS3，13-Surrogate，14-bisphenol A\n","description":"","filename":"f1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-195989/v1/8f2042587e38a29400ac3375.jpg"},{"id":13682934,"identity":"a43e1c7f-09b4-43c3-9672-d89e98a2ad6a","added_by":"auto","created_at":"2021-09-17 11:59:46","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":407424,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-195989/v1/f1244ff7-fce4-4b9c-a7f4-95d0af2da6af.pdf"},{"id":7234737,"identity":"6c689e84-60b6-48df-9e25-183a3695d81e","added_by":"auto","created_at":"2021-03-22 19:03:44","extension":"docx","order_by":5,"title":"","display":"","copyAsset":false,"role":"supplement","size":349053,"visible":true,"origin":"","legend":"","description":"","filename":"GraphicalAbstract.docx","url":"https://assets-eu.researchsquare.com/files/rs-195989/v1/696a21cb943af4ca1f135ef9.docx"}],"financialInterests":"","formattedTitle":"Simultaneous determination of C4-C9 alkylphenols and bisphenol A in environmental water at the Yellow River Estuary Area in China by gas chromatography-mass spectrometry","fulltext":[{"header":"Introduction","content":"\u003cp\u003eAs the typical environmental endocrine disruptors, alkylphenols and bisphenol A have been the focus of attention (Wen et al. 2020; Abu-Alsoud and Bottaro 2021; Liu and Zhao 2020; Murray et al. 2017). There is no domestic standard method to monitor the alkylphenols and bisphenol A in water in China. ASTM, ISO and the Japanese government have developed the corresponding monitoring standard methods (ISO 18857-2 2009; ASTM D7065 2011; ASTM D7574 2009; JIS K 0450-10-10 2006). However, these above monitoring methods were developed early and the research objects are single, which are limited to one or several compounds of 4-tert-octylphenol, nonylphenol and bisphenol A. This is related to the early people's understanding of the use of alkylphenols. The plastic industry developed early, and the nonylphenol and octylphenol were the most widely used in the plastic industry. As a result, the proportion of nonylphenol and octylphenol in the environmental water was high, and the other alkylphenols were not detected or the detection value was very low, which did not attract people's attention. There are many detection methods of alkylphenols and bisphenol A in water, among which the liquid-liquid extraction combined GC-MS method is a more studied method, but the research objects are also concentrated on one or several compounds of 4-tert-octylphenol, nonylphenol and bisphenol A (Selvaraj et al. 2014; Shen et al. 2015; \u003ca href=\"https://www.sciencedirect.com/science/article/abs/pii/S0045653511013555#!\"\u003eSanthi\u003c/a\u003e et al. 2012; Dong et al. 2015; Wang et al. 2013; Martinez and Pe\u0026ntilde;uela 2013). However, with the development of new plastics and liquid crystal products, many new C4-C9 alkylphenols have been widely used. The 4-tert-butylphenol is an important raw material for the production of p-tert-butylphenol formaldehyde resin. The 4-n-pentylphenol is an effective component of disinfectant, food preservative and deodorant. The 4-n-butylphenol, 4-n-hexylphenol and 4-n-heptylphenol are often used as liquid crystal raw materials and intermediates. The wide application of C4-C9 alkylphenols leads to the continuous entry of these new alkylphenols into the environmental water. These C4-C9 alkylphenols are also the endocrine disruptors, which can also affect the performance of human endocrine system. In 1998, the Ministry of environment of Japan listed 4-pentyl phenol and other alkyl phenols as the endocrine disruptors (Ministry of the Environment of Japan 1998). However, there is no standard method for the monitoring of these C4-C9 alkylphenol and bisphenol A in water in China, and there is no standard method developed by the authoritative institutions abroad.\u003c/p\u003e\n\u003cp\u003eThe alkylphenol is a combination of phenol and alkyl chain. Due to the different length of alkyl chain, the physical and chemical properties of these C4-C9 alkylphenols are different, such as nonpolarity, adsorption capacity on the material surface and partition coefficient between aqueous phase and organic phase. The greater the difference of alkyl chain length, the greater the difference of these properties. The quantitative determination of these C4-C9 alkylphenols and bisphenol A in groundwater, surface water, sewage and wastewater, seawater and other actual water samples is an analytical technique for trace or ultra trace mixed components in complex matrix, which is difficult to extract, enrich, separate and detect. Liquid-liquid extraction has the advantages of good separation effect, simple operation and high repeatability, which is the most widely used organic matter extraction and enrichment technology (Wang et al. 2020; Deng et al. 2018). GC-MS is the most advanced and reliable detection technology (Subuhi et al. 2020; Azzouz et al. 2020). After the derivatization, the polarity and boiling point of these alkylphenols and bisphenol A decreased, the volatility and stability increased, the anti-interference ability increased, and the samples could be stored for a long time for retest (Liu et al. 2019; Tan et al. 2019).\u003c/p\u003e\n\u003cp\u003eIn this study, a liquid-liquid extraction combined with derivatization and GC-MS method with low detection limit, high recovery, good reproducibility and rapid analysis was established. Nine C4-C9 alkylphenols (4-tert-butyl phenol, 4-n-butyl phenol, 4-n-pentyl phenol, 4-hexyl phenol, 4-heptyl phenol, 4-octyl phenol, 4-tert-octyl phenol, 4-nonylphenol and 4-nonylphenol) and bisphenol A in water were analyzed. The accurate concentrations of target substances in groundwater, surface water, sewage, waste water and sea water were determined.\u003c/p\u003e"},{"header":"Materials And Methods","content":"\u003cp\u003e\u003cstrong\u003e1.1 Materials and reagents\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe standard solutions of C4-C9 alkylphenols and bisphenol A (including 4-tert-butyl phenol, 4-n-butyl phenol, 4-n-pentyl phenol, 4-n-hexyl phenol, 4-n-heptyl phenol, 4-n-octyl phenol, 4-tert-octyl phenol, 4-n-nonylphenol, 4-nonylphenol and bisphenol A, with the concentration of 1000 \u0026mu;g/ml), deuterated bisphenol A-d\u003csub\u003e16\u003c/sub\u003e standard solution (1000 \u0026mu;g/ml), deuterated naphthalene-d\u003csub\u003e8\u003c/sub\u003e standard solution (200 \u0026mu;g/ml), deuterated phenanthrene-d\u003csub\u003e10 \u003c/sub\u003estandard solution (500 \u0026mu;g/ml), and deuterated pyrene-d\u003csub\u003e10\u003c/sub\u003e standard solution (500 \u0026mu;g/ml), were purchased from Drehrenstorfer, Germany. Dichloromethane, acetone and n-hexane are pesticide residues, purchased from Merda company of USA. The derivatization reagent BSTFA (containing 1% TMCs) was purchased from Drehrenstorfer, Germany. Hydrochloric acid, sodium chloride and anhydrous sodium sulfate are of high grade purity purchased from Sinopharm group of China. Sodium chloride and anhydrous sodium sulfate are roasted at 450 ℃ .\u003c/p\u003e\n\u003cp\u003eAlkylphenols and bisphenol A working solution (1 \u0026mu;g/ml), substitute solution (1 \u0026mu;g/ml of deuterated bisphenol A-d\u003csub\u003e16\u003c/sub\u003e), and internal standard (1 \u0026mu;g/ml of deuterated naphthalene-d\u003csub\u003e8\u003c/sub\u003e, deuterated phenanthrene-d\u003csub\u003e10\u003c/sub\u003e, and deuterated pyrene-d\u003csub\u003e10\u003c/sub\u003e, respectively) were diluted from the corresponding standard solutions with acetone.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.2 Instruments and equipment\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAgilent 7890B gas chromatograph, Agilent 5977B mass spectrometer, Heidolph rotary evaporator.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.3 Solution preparation and standard curve\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWater treatment steps: Measured 500 ml water sample into the separating funnel, added 100 \u0026micro;l substitute working solution and mixed well. Then added 10 g sodium chloride, shaked to complete dissolution, added 30 ml dichloromethane, shaked violently for 10 min, standed for 5-10 min, separated layers, collected organic phase, extracted again for 1-2 times, combined organic phase and dehydrated with anhydrous sodium sulfate, and concentrated organic phase to 0.5 ml. Transfered the concentrated sample to a 1 ml volumetric flask, washed the concentrated flask with a small amount of dichloromethane, and combined the washing solution into the volumetric flask. Added 100 \u0026micro;l of internal standard working solution and 100 \u0026micro;l of derivatization reagent in turn, and diluted to 1 ml with dichloromethane. The samples were obtained by derivatization at room temperature for 1 h.\u003c/p\u003e\n\u003cp\u003eStandard curve preparation: Added 100 \u0026mu;l internal standard working solution and 100 \u0026mu;l derivatization reagent respectively, and used dichloromethane to make volume to 1 ml. The low concentration calibration series are 0 \u0026mu;g/L, 5.0 \u0026mu;g/L, 10.0 \u0026mu;g/L, 20.0 \u0026mu;g/L, 40.0 \u0026mu;g/L, 60.0 \u0026mu;g/L and 80.0 \u0026mu;g/L, while the high concentration calibration series are 60.0 \u0026mu;g/L, 100 \u0026mu;g/L, 200 \u0026mu;g/L, 500 \u0026mu;g/L, 1000 \u0026mu;g/L and 2000 \u0026mu;g/L. After derivatization at room temperature for 1 h, the target compounds were determined from low concentration to high concentration according to the chromatographic reference conditions. The retention time and quantitative ion response values of the standard series of target compounds and corresponding internal standards were recorded. The average relative response factor was used to draw the calibration curve, and the relative standard deviation (RSD) of the relative response factor (RRF) of the standard series of target compounds should not be more than 20%.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.4 Actual water sample collection\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAccording to the relevant environmental protection standards (HJ 91.1 2019; HJ/T 164 2020; HJ/T 493 2009), the sewage, river water, the drainage of wastewater treatment plant and Bohai Sea water were collected in Dongying City. The hydrochloric acid solution was added to adjust the pH of the water sample to be less than or equal to 2. The water sample should be filled with the sample bottle and sealed. It should be stored under 4 ℃ in dark and refrigerated. The extraction and derivation should be completed within 10 days, and the analysis should be completed within 5 days after the extraction.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e1.5 Establishment of analytical method\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e(1) Gas chromatographic conditions\u003c/p\u003e\n\u003cp\u003eInjection port temperature: 300 ℃, no split injection. He gas pressure: 40 kPa for 5 min, 2 kPa/min to 70 kPa, 5 min; column temperature: 50 ℃, 2 min, 20 ℃/min to 100 ℃, 10 ℃/min to 200 ℃, 20 ℃/min to 300 ℃, 5 min; injection volume: 1 \u0026mu;l. The chromatographic column was hp-5ms capillary column, 30 m long, 0.25 mm inner diameter and 0.25 \u0026mu;m film thickness.\u003c/p\u003e\n\u003cp\u003e(2) Mass spectrometry conditions\u003c/p\u003e\n\u003cp\u003eTransmission line temperature: 280 ℃. Quadrupole temperature: 150 ℃. Ion source temperature: 230 ℃. Electron energy of ion source: 70 ev. Mass range: 35-400 amu. Data acquisition mode: SIM. The quantitative and qualitative ions are shown in Table 1.\u003c/p\u003e\n\u003cp\u003eTable 1 Quantitative ion and qualitative ion of target compounds\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026nbsp;Compound Name\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eCAS number\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eQuantitative ion\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eQualitative ion\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eNote\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003edeuterated naphthalene-d\u003csub\u003e8\u003c/sub\u003e（IS 1）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1146-65-2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e136\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026mdash;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026mdash;\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98-54-4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e207\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e222\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1638-22-8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e179\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e222\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e14938-35-3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e179\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e236\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2446-69-7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e179\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e250\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e140-66-9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e207\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e278\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1987-50-4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e179\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e264\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e84852-15-3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e207\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e221、193\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003edeuterated phenanthrene-d\u003csub\u003e10\u003c/sub\u003e（IS 2）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1517-22-2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e188\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026mdash;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026mdash;\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1806-26-4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e179\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e278\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104-40-5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e179\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e292\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003edeuterated pyrene-d\u003csub\u003e10\u003c/sub\u003e （IS 3）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1718-52-1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e212\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026mdash;\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026mdash;\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003edeuterated bisphenol A-d\u003csub\u003e16\u003c/sub\u003e（Surrogate）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96210-87-6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e368\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e386\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 3\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e80-05-7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e357\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e372\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eIS 3\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e"},{"header":"Results And Discussion","content":"\u003cp\u003e\u003cstrong\u003e2.1 Influence of extraction solvent\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e500 ml of laboratory water was added into the separating funnel, and then alkylphenol and bisphenol A working solution were added to make the concentration of target substance in the water sample 0.20 \u0026micro;g/L. The effects of different extraction solvents on the recovery of target compounds are shown in Table 2. According to the data in the table, dichloromethane has the best extraction effect.\u003c/p\u003e\n\u003cp\u003eTable 2 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction solvents\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eExtraction solvent\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRecovery rate %\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDichloromethane\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e120\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.8\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eHexane\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e118\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e13.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e83.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e94.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e87.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eEthyl acetate\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e91.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e84.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e83.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e65.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e72.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e80.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e65.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e82.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e66.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e76.2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eToluene\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e65.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e88.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e65.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e78.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e72.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e83.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e79.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e88.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e75.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e55.1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.2 Influence of extraction times\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe effects of different extraction times of dichloromethane on the recovery of target compounds are shown in Table 3. It can be seen from the table that C4-C9 alkylphenols can be extracted completely after one extraction, and bisphenol A can be extracted mainly after two extraction. When dichloromethane is selected as the extraction solvent, the second extraction can meet the requirements.\u003c/p\u003e\n\u003cp\u003eTable 3 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction times\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026nbsp;Extraction times\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRecovery rate %\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e119\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e76.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e62.6\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e24.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e27.4\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5.2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 Influence of extraction time\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe influence of different extraction time on the recovery rate of target compounds is shown in Table 4. It can be seen that the extraction time has a slight effect on the extraction efficiency. With the extension of the extraction time, the recovery rate of the target substance increases slightly. It can be seen from the table that the extraction time of 5 minutes is enough to fully recover the target substance in the water sample.\u003c/p\u003e\n\u003cp\u003eTable 4 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction time\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eExtraction time（min）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRecovery rate %\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e118\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e91.0\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e10\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e119\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e90.0\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e15\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e118\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e122\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e127\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e123\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e92.3\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.4 Influence of acidity in water\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe effect of acidity in water on the recovery rate of target compounds is shown in Table 5. It can be seen from the table that when the pH value is higher than 6, the recoveries of 4-nonylphenol and 4-n-octyl phenol decrease, but the recoveries of other target compounds are not affected. Considering that acidic conditions are conducive to inhibiting bacteria in water and preventing bacteria from consuming alkylphenol organics, the pH value of water sample is usually less than 2.\u003c/p\u003e\n\u003cp\u003eTable 5 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different pH value\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003epH 值\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026lt;1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1-2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2-3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3-4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e6-7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.8\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e122\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e125\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e120\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e114\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e105\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e105\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e105\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e91.3\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e116\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e121\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e121\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e120\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e116\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e114\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e121\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e114\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.5 Influence of extraction solvent volume\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe effects of different extraction solvent volumes on the recovery of target compounds are shown in Table 6. It can be seen from the table that 20 ml of dichloromethane can meet the extraction requirements.\u003c/p\u003e\n\u003cp\u003eTable 6 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different extraction solvent volume\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eExtraction solvent volume（ml）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRecovery rate %\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e116\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e30\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e116\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e118\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e40\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e118\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e117\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e50\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e121\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e122\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.6 Influence of salt content\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003ePhenols are water-soluble and need salting out to improve extraction efficiency. The effect of different salt amount on the recovery rate of target substance is shown in Table 7. It can be seen from the table that adding sodium chloride can indeed improve the recovery rate of the target substance, but the improvement effect is not significant. The main reason is that the recovery rate data is high enough without adding salt. After comprehensive consideration, 5g sodium chloride can be added in water sample extraction.\u003c/p\u003e\n\u003cp\u003eTable 7 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different salt content\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eNaCl Dosage (g)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e30\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e114\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e114\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e93.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e105\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e105\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e113\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e114\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e109\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e108\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.7 Effect of drying time during concentration process\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn the standard of organic matter determination in water, when liquid-liquid extraction is used, it is related to whether the extraction liquid is dried during the concentration process. For C4-C9 alkylphenols, some of the target compounds are liquid with vapor pressure, and volatilization occurs when the vacuum is appropriate. Some of the solid targets are powder or velvet with low density, and they also have drift loss when the vacuum is appropriate. The influence of organic phase concentration to 0.5 ml or concentration to dryness and continuous rotary evaporation for different time on the recovery of target substance is shown in Table 8. It can be seen from the table that drying can significantly reduce the detection results, especially for some alkylphenols (4-tert-butylphenyl, etc.) which are liquid at room temperature. The experimental results are consistent with the above analysis. Finally, in the process of concentration, the treatment mode without drying was selected.\u003c/p\u003e\n\u003cp\u003eTable 8 Average extraction efficiency of C4-C9 alkylphenols and bisphenol A for different drying process\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDrying time（min）\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eCondense to 0.5 ml\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e40.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e21.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e20.9\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e54.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e22.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e18.8\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e73.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e32.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e23.2\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e84.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e56.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e44.4\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e103\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e88.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e60.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e53.7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e90.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e79.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e74.4\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e92.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e82.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e74.9\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e90.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e87.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e84.7\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e94.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e92.6\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e93.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e94.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e92.1\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e92.9\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.8 Spectrogram\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe chromatogram of the standard sample of the target analytes is shown in Fig 1. It can be seen from the figure that under the conditions of reference gas chromatography-mass spectrometry, the peaks of each compound are independent and clear. Due to the use of derivatization treatment, there is basically no tailing phenomenon of phenolic compounds. 4-nonylphenol is a mixed peak of one group of isomers, and other compounds are single peaks.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.9 Standard curve\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe linear correlation coefficient of the standard curve of the object to be measured is shown in Table 9. It can be seen from the table that the standard curves of the target compounds are linear.\u003c/p\u003e\n\u003cp\u003eTable 9 Linear correlation coefficient, detection limit and lower limit of determination of C4-C9 alkylphenols and bisphenol A\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eRSD of RRF\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eLow concentration curve\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e12.2%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e6.02%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e6.21%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e6.30%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5.85%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e6.71%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e8.00%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4.04%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4.31%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3.05%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eHigh concentration curve\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.71%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3.42%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4.87%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e7.03%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5.36%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10.7%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3.18%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e9.64%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e9.64%\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e6.47%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003edetection limit(\u0026mu;g/L)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.006\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eLower limit of determination(\u0026mu;g/L)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.024\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.012\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.012\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.10 Method detection limit\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe detection limit of the method was confirmed according to the relevant environmental protection standard method (HJ 168 2020), and seven laboratory blank samples with or without the target objects working solution were analyzed continuously. Because 4-tert-butylphenyl, 4-tert-octylphenyl, 4-nonylphenol and bisphenol A were detected in the laboratory blank, the detection limits of these target substances were determined by the method of laboratory blank without standard addition and the detection limits of the remaining analytes were determined by the method of laboratory blank with standard addition. The concentration of these remaining analytes in the laboratory blank sample with standard addition is all 0.010 \u0026mu;g/L. The lower limit of determination is 4 times of the detection limit and both of which are listed in Table 9. These detection limits of the target compounds changes from 0.002 \u0026mu;g/L to 0.006 \u0026mu;g/L and the lower limit of determination from 0.008 \u0026mu;g/L to 0.024 \u0026mu;g/L.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.11 Recovery and precision\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTaking the laboratory water without the target analytes as the blank matrix, C4-C9 alkylphenols and bisphenol A standard solutions with three different concentration levels were added respectively. The pretreatment and test were carried out according to the method steps. Each concentration level was determined for six times in parallel. The relative standard deviation results are shown in table 10. Take surface water, sewage and wastewater as sample matrix, add C4-C9 alkylphenols and bisphenol A standard solution with matrix concentration level of 0.5-3 times, repeat the above laboratory test, and the average recovery results are shown in Table 11. The relative standard deviations of the target samples ranged from 0.67% to 13.7%, and the average recovery ranged from 68.0% to 122%, which indicates that the precision and accuracy of the experiment are good.\u003c/p\u003e\n\u003cp\u003eTable 10 Precision datas\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSpiked concentration\u003c/p\u003e\n\u003cp\u003e(\u0026micro;g/L)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRelative standard deviation%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e0.02\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e9.52\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4.62\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3.93\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.90\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.68\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4.69\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e12.3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e7.08\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e0.50\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3.20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.15\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.88\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.92\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.96\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.79\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.49\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.29\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e3.12\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.44\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e2.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.05\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.42\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.12\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.19\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.51\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.74\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5.32\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e7.20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e13.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.67\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eTable 11 Recovery datas\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWater sample\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSpiked concentration(\u0026micro;g/L)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRecovery rate of standard addition %\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSewage\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.05\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e106\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e96.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e101\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e90.7\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e112\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurface water\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.50\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e99.5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e116\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e121\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e122\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e72.4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e79.1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e88.2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e68.0\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWastewater\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e95.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e107\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e111\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.9\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e102\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e98.6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e91.4\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003e2.12 Determination of typical representative water samples\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe contents of target analytes in groundwater, surface water, sewage, wastewater and seawater samples are shown in Table 12. It can be seen from the table that the contents of 4-nonylphenol and bisphenol A in the environmental water in Dongying City are below the requirements of relevant directives at home and abroad (GB 31572 2015; GB 31571 2015; U.S. EPA 2005; \u003ca href=\"https://www.researchgate.net/scientific-contributions/European-Commission-EC-2112771433\"\u003eEuropean Commission EC\u003c/a\u003e 2003), and the contents of target substances in groundwater, surface water and seawater are very low.\u003c/p\u003e\n\u003cp\u003eTable 12 The measured value of target analytes in actual water sample（\u0026micro;g/L）\u003c/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-butyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-pentyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-hexyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-tert-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-heptyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-octyl phenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e4-n-nonylphenol\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003ebisphenol A\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSurrogate\u003c/p\u003e\n\u003cp\u003eRecovery\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eGroundwater\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.016\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e124%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurface water 1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.049\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.023\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e125%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurface water 2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.026\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.032\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e78.6%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurface water 3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.031\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.007\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.139\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.068\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e92.3%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSurface water 4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.228\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.226\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e77.4%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSewage 1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.004\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.019\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.965\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.024\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e110%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSewage 2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.344\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.156\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e88.5%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWastewater 1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.007\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.37\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.200\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e115%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWastewater 2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.207\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.029\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e81.9%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWastewater 3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1.81\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e2.30\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e70.5%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWastewater 4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.006\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.004\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.005\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.007\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.016\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.017\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.218\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.064\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.094\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.870\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e97.9%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eWastewater 5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.004\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.009\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.114\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.026\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.035\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e32.8\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e87.3%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eSeawater\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.003\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.077\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003en.d.\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.011\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd\u003e\n\u003cp\u003e104%\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003eNote: surface water 1 is drinking water, \u003c/sup\u003e\u003csup\u003esurface water 2 is yellow river water, surface water 3 is Guangli River water, surface water 4 is Xiaoqing River water, sewage 1 is outlet water of a sewage treatment plant in Dongying City, sewage 2 is outlet water of a sewage treatment plant in Guangrao County, Dongying City, wastewater 1 is outlet water of an industrial wastewater treatment plant in Dongying City, and wastewater 2 is outlet water of an oil processing enterprise in Dongying City, wastewater 3 is from the workshop of an oil processing enterprise in Dongying City, wastewater 4 is from the workshop of a bisphenol A production enterprise in Dongying City, and wastewater 5 is from the workshop of a bisphenol A production enterprise in Dongying City.\u003c/sup\u003e\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003e(1) Aiming to monitor of C4-C9 alkylphenols and bisphenol A in water, A liquid-liquid extraction combined with derivatization and gas chromatography-mass spectrometry (GC-MS) method was used to optimize the extraction solvent, extraction times, extraction time, extraction solvent volume, acidity, salt content, rotary distillation concentration degree and other conditions. After optimization, dichloromethane was used as the extraction solvent, the extraction time was 5 min, the extraction solvent was 30 ml, the pH value was less than 2, and the amount of salt was 5 g.\u003c/p\u003e\n\u003cp\u003e(2) The method has the advantages of high sensitivity, low detection limit, accurate quantification and good precision, which can meet the needs of the determination of C4-C9 alkylphenols and bisphenol A in water.\u003c/p\u003e\n\u003cp\u003e(3) The method was used to detect the groundwater, surface water, sewage, wastewater and sea water samples in Dongying City located at the Yellow River Estuary. The results showed that the contents of 4-nonylphenol and bisphenol A in the samples were lower than the requirements of relevant directives at home and abroad.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgments \u003c/strong\u003eWe appreciate the help of the Ecological and Environmental Monitoring Center of Dongying Municipal of Shandong Province in sample testing.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; contributions \u003c/strong\u003eZiqi Guo, Xinliang Liu and Jian Zhao carried out the experiment. Hailiang Yin and Tongna Zhou put forward the idea. All authors have read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding information \u003c/strong\u003eThis work was supported by Standard Development and Revision Project of Ministry of Ecological Environment of China (Grant Nos. 2014-31) , Shandong Provincial Natural Science Foundation, Chian（ZR2019MB022）and the Fundamental Research Funds for the Central Universities (19CX02063A).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials \u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompliance with ethical standards\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate \u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication \u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests \u003c/strong\u003eThe authors declare that they have no competing interests.\u003c/p\u003e"},{"header":"References","content":"\u003cp\u003eAbu-Alsoud GF, Bottaro CS (2021) Porous thin-film molecularly imprinted polymer device for simultaneous determination of phenol, alkylphenol and chlorophenol compounds in water. 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J Sep Sci 42(5):991-998\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Environmental water, C4-C9 alkylphenol, Bisphenol A, Gas chromatography-mass spectrometry, Liquid-liquid extraction, Yellow River Estuary area","lastPublishedDoi":"10.21203/rs.3.rs-195989/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-195989/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eA liquid-liquid extraction combined with derivatization and gas chromatography-mass spectrometry (GC-MS) method was developed for the rapid determination of C4-C9 alkylphenols (4-tert-butylphenol, 4-n-butylphenol, 4-n-pentylphenol, 4-n-hexylphenol, 4-n-heptylphenol, 4-n-octylphenol, 4-tert-octylphenol, 4-n-nonylphenol and 4-nonylphenol) and bisphenol A in the environmental water. The extraction solvent, extraction time, solvent volume, acidity, salt content and the distillation degree were optimized. After drying and dehydration, the extract was derivatized, detected by GC-MS and quantified by internal standard method. After the dehydration, the extract was derivatized with BSTFA, detected by GC-MS and quantified by internal standard method. The results showed that the relative standard deviations of the relative response factors of the target compounds were less than 20%, the method detection limits were 0.002 \u0026micro;g/Lཞ0.006 \u0026micro;g/L, the relative standard deviations of the three spiked levels were 0.67%ཞ13.7%, and the average recoveries of the actual water samples were 68.0%ཞ122%. The method precision and accuracy were good. The method is simple, rapid, accurate, stable and reliable, which is suitable for the detection of target in water. The contents of C4-C9 alkylphenols and bisphenol A in groundwater, surface water, sewage, waste water and sea water samples in Dongying City located at the Yellow River Estuary were determined by this method. The results showed that the contents of 4-nonylphenol and bisphenol A in these samples were lower than the home and abroad requirements.\u003c/p\u003e","manuscriptTitle":"Simultaneous determination of C4-C9 alkylphenols and bisphenol A in environmental water at the Yellow River Estuary Area in China by gas chromatography-mass spectrometry","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-03-22 19:00:41","doi":"10.21203/rs.3.rs-195989/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"0af0bff3-b888-495f-9bd7-d969850e04a4","owner":[],"postedDate":"March 22nd, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":3144361,"name":"Environmental Engineering"}],"tags":[],"updatedAt":"2021-04-21T08:42:02+00:00","versionOfRecord":[],"versionCreatedAt":"2021-03-22 19:00:41","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-195989","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-195989","identity":"rs-195989","version":["v1"]},"buildId":"WvIrzKhiLBfengagbw6Ux","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}