Deposition of Daldinia starbaeckii (ELF) functionalized silver nanoparticles on Urinary catheter tube using chitosan polymer to prevent bacterial biofilms formation during UTI infection

Catheter-associated urinary tract infections (CAUTI) are the most common healthcare problem in hospitals. In this study, we isolated and the Daldinia starbaeckii (An endolichenic fungus from Roccella montagnie ) and its biomass extract was used to simultaneously synthesize and deposit DSFAgNPs on the inner and outer surfaces of the catheter tube using chitosan biopolymer via In-Situ deposition method. Perfectly designed D. starbaeckii extract functionalized DSFAgNPs were characterized by UV spectroscopy, FTIR, SEM, EDS, TEM, and XRD. The microbial efficacy of DSFAgNPs & DSFAgNPs coated catheter (CTH3) was evaluated against nine human pathogenic gram (+/-) ive strains. Results indicated DSFAgNPs showed significant biological activity against both gram (+/-) ive bacteria with an average MIC of 4µl/ml. The most promising activity was observed against Helicobacter pylori . CTH3 treated bacterial strains showed a significant reduction in colony formation unit (CFU/ml) in broth culture assay. Antibiofilm activity of CTH3 showed strong inhibition of biofilm formation (85%).