Single-cell RNA sequencing revealed the T cell heterogeneity and exhaustion subsets of Helicobacter pylori-induced gastritis - cancer transformation

Single-cell RNA sequencing revealed the T cell heterogeneity and exhaustion subsets of Helicobacter pylori-induced gastritis - cancer transformation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL View This is a preprint and has not been peer reviewed. Data may be preliminary. 20 June 2025 V1 Latest version Share on Single-cell RNA sequencing revealed the T cell heterogeneity and exhaustion subsets of Helicobacter pylori-induced gastritis - cancer transformation Authors : Yanhui Yang , Yanxia Liu , Xiaole Guo , Zhaorun Li , Ziyu Liu , Shuming Zhang , Yuma Yang , … Show All … , Jun Liu , Shoucheng Ma , Linke Ma , Xianmei Chen , Xue Li , Yuanyuan Tang , Xiaoling Ding , Xiaofei Li , Qian Hao , Yanjie You , Tingting Yang , Qinrui Li , Xue Zhang , Sihan Zhang , Hengjun Gao , and Shengjuan Hu 0009-0006-3237-7242 [email protected] Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.175041561.11656852/v1 Published VIEW Version of record Peer review timeline 323 views 120 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background Gastric cancer (GC) is the third leading cause of cancer-related deaths worldwide. Helicobacter pylori (H. pylori) infection is a crucial initiative factor for the Correa cascade occurrence of GC. However, the immunological mechanisms of H. pylori-induced GC remain incompletely understood, particularly at the single-cell RNA sequencing (scRNA-seq) level. Methods We established a scRNA-seq atlas of gastric tumor, with a focus on T-cell-related genes for trajectory analysis,cell-cell communication analysis,and transcription factor regulatory network analysis. The results were validated by immunofluorescence staining of gastric tumors. Results We collected 63,603 cells from ten human gastric tissues and identified 20 distinct clusters, annotating 14 cell types based on canonical markers.In-depth dissection disclosed seven T cell subtypes in H. pylori positive gastric tumors. We further revealed two types of exhausted T cells CD4-C2-TIGIT(Tex)and CD8-C3-PDCD1(Tex). Among them, CD4-C2-TIGIT accounted for the highest proportion in H. pylori positive GC, from which we screened that the gene FYB1 was significantly correlated with prognosis. Immunofluorescence staining verified the localized expression of FYB1 in the T cell-rich areas of H. pylori positive tumors, further substantiating their role in the tumor immune landscape. Moreover, network interaction analysis demonstrated that immune cells, stromal cells, and epithelial cells interact in the tumor microenvironment (TME). Conclusion Our study constructs a comprehensive T cell atlas of GC, delineating T cell heterogeneity and their functional interactions within the H. pylori-driven TME. These findings highlight the prognostic potential of FYB1 in tumor immunity and provide new insights into the immunological mechanisms driving GC progression. Supplementary Material File (figure.docx) Download 2.37 MB File (manuscript.doc) Download 333.50 KB Information & Authors Information Version history V1 Version 1 20 June 2025 Peer review timeline Published VIEW Version of Record 27 Nov 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection View Keywords fyb1 gastric carcinogenesis helicobacter pylori immune microenvironment scrna-seq Authors Affiliations Yanhui Yang Ningxia Medical University View all articles by this author Yanxia Liu Ningxia Medical University View all articles by this author Xiaole Guo People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Zhaorun Li Fudan University Shanghai Cancer Center View all articles by this author Ziyu Liu National Engineering Research Center for Biochip at Shanghai View all articles by this author Shuming Zhang Ningxia Medical University View all articles by this author Yuma Yang Ningxia Medical University View all articles by this author Jun Liu People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Shoucheng Ma People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Linke Ma People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Xianmei Chen People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Xue Li People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Yuanyuan Tang People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Xiaoling Ding People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Xiaofei Li People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Qian Hao People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Yanjie You People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Tingting Yang Ningxia Medical University View all articles by this author Qinrui Li Ningxia Medical University View all articles by this author Xue Zhang Ningxia Medical University View all articles by this author Sihan Zhang Ningxia Medical University View all articles by this author Hengjun Gao Tongji University School of Medicine View all articles by this author Shengjuan Hu 0009-0006-3237-7242 [email protected] People's Hospital of Ningxia Hui Autonomous Region View all articles by this author Metrics & Citations Metrics Article Usage 323 views 120 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Yanhui Yang, Yanxia Liu, Xiaole Guo, et al. Single-cell RNA sequencing revealed the T cell heterogeneity and exhaustion subsets of Helicobacter pylori-induced gastritis - cancer transformation. Authorea . 20 June 2025. DOI: https://doi.org/10.22541/au.175041561.11656852/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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