Effects of p38 MAPK pathway inhibition on the metabolism of periodontal ligament fibroblasts during inflammation

Effects of p38 MAPK pathway inhibition on the metabolism of periodontal ligament fibroblasts during inflammation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Effects of p38 MAPK pathway inhibition on the metabolism of periodontal ligament fibroblasts during inflammation Cheng Qian, Yifan Gu, Feiyan Gao, Zenan Xu, Jiahui Li, Rui He, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6357141/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Transcription factor - related enzymes are involved in the inflammatory feedback of metabolic recombination. We aimed to understand how p38 MAPK deficiency impacts metabolic function in inflamed hPDLFs treated with P.g - LPS. CCK − 8, cell scratches, in vitro immunohistochemistry, etc. were used. A p38 MAPK inhibitor regulated cellular metabolism, and real - time PCR and western blot analyzed the P38 MAPK pathway's effect on metabolism. P.g - LPS - treated hPDLFs had metabolic reconfiguration with increased MMP − 1, 2, 3. Adding p38 MAPK inhibitors lessened P.g - LPS's harm and decreased metabolic recombination. Phosphorylated MMPs and p38 MAPK expression were reduced in inhibitor - treated hPDLFs. Blocking the P38 MAPK pathway reduces LPS - induced inflammation by altering metabolism, offering a new periodontal therapy approach. Biological sciences/Cell biology Biological sciences/Molecular biology Health sciences/Medical research Health sciences/Pathogenesis Metabolism p38 MAPK pathway P.gingivalis Periodontal inflammation Matrixmetallo proteinases Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6357141","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":452794821,"identity":"cda36b2e-b8d8-4a6e-a8d3-6dd630015e9a","order_by":0,"name":"Cheng Qian","email":"","orcid":"","institution":"SHAOXING CENTRAL HOSPITAL","correspondingAuthor":false,"prefix":"","firstName":"Cheng","middleName":"","lastName":"Qian","suffix":""},{"id":452794822,"identity":"f8f6b704-bb2b-4b52-8a49-5c8f95197b3a","order_by":1,"name":"Yifan Gu","email":"","orcid":"","institution":"SHAOXING KEQIAO WOMEN\u0026CHILDREN'S 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We aimed to understand how p38 MAPK deficiency impacts metabolic function in inflamed hPDLFs treated with P.g - LPS. CCK \u0026minus;\u0026thinsp;8, cell scratches, in vitro immunohistochemistry, etc. were used. A p38 MAPK inhibitor regulated cellular metabolism, and real - time PCR and western blot analyzed the P38 MAPK pathway's effect on metabolism. P.g - LPS - treated hPDLFs had metabolic reconfiguration with increased MMP \u0026minus;\u0026thinsp;1, 2, 3. Adding p38 MAPK inhibitors lessened P.g - LPS's harm and decreased metabolic recombination. Phosphorylated MMPs and p38 MAPK expression were reduced in inhibitor - treated hPDLFs. 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