An Efficient And Quick Protocol For In Vitro Multiplication Of Snake Plant, Sansevieria Trifasciata Var. Laurentii [Prain]

Abstract An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments were surface sterilized and inoculated on Murashige and Skoog media supplemented with 3% sucrose, 0.8% agar, and various concentrations of indole-3-butyric acid. Cultures were maintained for 4-5 weeks at standard conditions to allow root induction and elongation. Shoot induction is triggered upon altering the culture room temperature to 37±2⁰C. Multiple shoots were witnessed at higher IBA concentrations. Another 5 weeks later, individual plantlets were excised and hardened for 2 weeks in soil preparation contained in small cups before transferring to big sized pots.