Mesoscopic elasticity controls dynamin-driven fission of lipid tubules

Mesoscopic elasticity controls dynamin-driven fission of lipid tubules | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Mesoscopic elasticity controls dynamin-driven fission of lipid tubules Carlo Casciola, Marco Bussoletti, Mirko Gallo, Matteo Bottacchiari, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4059226/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 10 You are reading this latest preprint version Abstract Mesoscale physics bridges the gap between the microscopic degrees of freedom of a system and its large-scale continuous behavior and highlights the role of a few key quantities in complex and multiscale phenomena, like dynamin-driven fission of lipid membranes. The dynamin protein wraps the neck formed during clathrin-mediated endocytosis, for instance, and constricts it until severing occurs. Although ubiquitous and fundamental for life, the cooperation between the GTP-consuming conformational changes within the protein and the mesoscale response of the underlying lipid substrate is yet to be unraveled. In this work, we build an effective mesoscopic model from constriction to fission of lipid tubules based on continuum membrane elasticity and implicitly accounting for ratchet-like power strokes of dynamins. Localization of the fission event, the overall geometry, and the energy expenditure we predict comply with the major experimental findings. This bolsters the idea that a mesoscopic continuous picture emerges soon enough to relate dynamin polymerization length and membrane rigidity and tension with the optimal pathway to fission. We therefore suggest that dynamins found in in vivo processes may optimize their structure accordingly. Ultimately, we shed light on real-time conductance measurements available in literature and predict the fission time dependency on elastic parameters. Physical sciences/Physics/Biological physics Physical sciences/Mathematics and computing/Applied mathematics Biological sciences/Biophysics/Computational biophysics Biological sciences/Biophysics/Membrane biophysics Canham Helfrich Phase field Fluid lipid bilayer Membrane fission Topological transition Full Text Additional Declarations No competing interests reported. Supplementary Files MesoscopicelasticitycontrolsdynamindrivenfissionoflipidtubulesNATCOMMSUBMISSIONSupplementary.pdf Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 25 Apr, 2024 Reviews received at journal 25 Apr, 2024 Reviews received at journal 04 Apr, 2024 Reviewers agreed at journal 24 Mar, 2024 Reviewers agreed at journal 24 Mar, 2024 Reviewers invited by journal 24 Mar, 2024 Editor assigned by journal 24 Mar, 2024 Editor invited by journal 24 Mar, 2024 Submission checks completed at journal 21 Mar, 2024 First submitted to journal 09 Mar, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4059226","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":283274861,"identity":"f4c85006-e512-48b7-9beb-bca2b9076eb1","order_by":0,"name":"Carlo 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