A fluorescent sensor for rapid and quantitative aquatic bacteria detection based on bacterial reactive oxygen species.

A fluorescent sensor for rapid and quantitative aquatic bacteria detection based on bacterial reactive oxygen species. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article A fluorescent sensor for rapid and quantitative aquatic bacteria detection based on bacterial reactive oxygen species. tingshan song, ruilin xiao, baohua tian, xinxin li, haixia liang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4768162/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 25 Oct, 2024 Read the published version in Microchimica Acta → Version 1 posted 14 You are reading this latest preprint version Abstract Determination of total number of bacteria is vital for water pollution monitoring. However, fast, portable and quantitative on-site aquatic bacteria detection remains challenging. Here, a fluorescence sensor for bacterial concentration detection based on Ag@carbon dots composites (Ag@CDs) were developed. Fluorescence of CDs was quenched by plasmon-enhanced resonance energy transfer from CDs to nano Ag and recovered due to interaction with bacteria. Ag@CDs with controllable fluorescence “OFF-ON” was prepared through regulation of Ag and CDs composition reaction. Ag+ concentration-dependent fluorescence quenching and bacterial concentration-dependent fluorescence recovery provided Ag@CDs the support for bacterial quantitative detection. After exposure to Ag@CDs, bacteria quickly produced ROS which remained unchanged at 5 min, that is the basis of rapid test of bacteria. Ag@CDs-based fluorescence sensing system was optimized and exhibited a wide linear range of 7×104~4×107 CFU/mL, low detection limit of 4×104 CFU/mL, short response time of 5 min and high selectivity. The 96-well plate assay for quantitative measurement and test strips assay for semi-quantitation were developed for rapid in-field aquatic bacteria detection. Therefore, this study could give a new sight for application of Ag@CDs for rapid on-site aquatic bacteria detection in water quality monitoring. Bacteria detection Fluorescence sensor Reactive oxygen species Ag nanoparticles Carbon dots Full Text Additional Declarations No competing interests reported. Supplementary Files SupportingInformation.docx sts.2021.7.16.3.tif sts.2021.7.19.5.tif Cite Share Download PDF Status: Published Journal Publication published 25 Oct, 2024 Read the published version in Microchimica Acta → Version 1 posted Editorial decision: Revision requested 17 Aug, 2024 Reviews received at journal 07 Aug, 2024 Reviews received at journal 03 Aug, 2024 Reviewers agreed at journal 31 Jul, 2024 Reviews received at journal 31 Jul, 2024 Reviewers agreed at journal 27 Jul, 2024 Reviews received at journal 25 Jul, 2024 Reviewers agreed at journal 25 Jul, 2024 Reviewers agreed at journal 25 Jul, 2024 Reviewers agreed at journal 25 Jul, 2024 Reviewers invited by journal 25 Jul, 2024 Editor assigned by journal 24 Jul, 2024 Submission checks completed at journal 24 Jul, 2024 First submitted to journal 19 Jul, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4768162","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":341385180,"identity":"e769924d-eb11-4efb-8600-8f6724a29d5b","order_by":0,"name":"tingshan song","email":"","orcid":"","institution":"Taiyuan University of Technology","correspondingAuthor":false,"prefix":"","firstName":"tingshan","middleName":"","lastName":"song","suffix":""},{"id":341385181,"identity":"6af9593f-1197-41f3-abf6-c7c023cf1ab0","order_by":1,"name":"ruilin xiao","email":"","orcid":"","institution":"Taiyuan University of 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