Preparation of Ca-Mg double-doped mesoporous silica nanoparticles and their drug-loading and drug-releasing properties

Preparation of Ca-Mg double-doped mesoporous silica nanoparticles and their drug-loading and drug-releasing properties | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Preparation of Ca-Mg double-doped mesoporous silica nanoparticles and their drug-loading and drug-releasing properties Qian Zhang, Jiamin Huang, Chao Liu, Ruihua Chen, Xiaohui Tang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4546457/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective: To prepare Doxorubicin-loaded Ca-Mg double-doped mesoporous silica nanoparticles (Ca-Mg@SiO 2 @DOX) and examine their properties. Methods: Mesoporous silica nanoparticles (MSNs) and Ca-Mg@SiO 2 nanoparticles were prepared by hydrothermal method and evaluated for particle size, zeta potential, morphology, and elemental distribution. SiO 2 @DOX nanoparticles and Ca-Mg@SiO 2 @DOX nanoparticles were prepared by electrostatic adsorption to investigate their properties, such as particle size, zeta potential, encapsulation efficiency, drug loading, stability, release degree, biosafety, and in vitro antitumor property. Results: MSNs and SiO 2 @DOX nanoparticles were successfully prepared with a particle size of about 200-300 nm and negatively charged; the MSNs and SiO 2 @DOX nanoparticles were regular spherical, and the Ca and Mg doped nanoparticles showed irregular spherical shape, and mapping measured the Ca and Mg elements contains were 2.92% and 1.19% respectively. SiO 2 @DOX nanoparticles and Ca-Mg@SiO 2 @DOX nanoparticles were successfully prepared with a particle size of about 300-400 nm, also negatively charged, encapsulation efficiency of about 90%, and a drug loading efficiency of 10%, with a slower release compared to DOX of solution. The release of Ca-Mg@SiO 2 @DOX nanoparticles was faster in an acid condition. All samples’ hemolysis efficiency was less than 5%. Cellular toxicity showed that SiO 2 @DOX nanoparticles and Ca-Mg@SiO 2 @DOX nanoparticles are toxic to lower than free DOX. Living and dead cell staining experiments showed similar situations. Cell uptake showed that cell uptake is higher compared with free DOX Ca-Mg@SiO 2 @DOX nanoparticles. Conclusion: We successfully prepared Ca-Mg@SiO 2 @DOX nanoparticles, which have a suitable particle size, zeta potential, and stability, which are released faster in acid conditions, which can reduce the toxicity of DOX, boost cell uptake, and have good antitumor properties. Ca-Mg double-doped mesoporous silica Adriamycin Nanoparticles Breast cancer Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4546457","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":320885673,"identity":"bc0ddf5f-f0e5-4cfb-8307-cde3a3aef66a","order_by":0,"name":"Qian Zhang","email":"","orcid":"","institution":"School of Pharmacy Xinjiang Medical University","correspondingAuthor":false,"prefix":"","firstName":"Qian","middleName":"","lastName":"Zhang","suffix":""},{"id":320885674,"identity":"facc30a4-a279-46f0-8136-350be0d6db65","order_by":1,"name":"Jiamin Huang","email":"","orcid":"","institution":"School of Pharmacy Xinjiang Medical 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13:36:08","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4546457/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4546457/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":65541968,"identity":"129725f6-2b81-43d6-8c7f-38db1fb03471","added_by":"auto","created_at":"2024-09-29 15:53:37","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1575593,"visible":true,"origin":"","legend":"","description":"","filename":"PreparationofCaMgdoubledopedmesoporoussilicananoparticlesandtheirdrugloadinganddrugreleasingproperties.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4546457/v1_covered_c1eb9fe1-82bd-4b15-8470-8359d1ea1a58.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Preparation of Ca-Mg double-doped mesoporous silica nanoparticles and their drug-loading and drug-releasing properties","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Ca-Mg double-doped mesoporous silica, Adriamycin, Nanoparticles, Breast cancer","lastPublishedDoi":"10.21203/rs.3.rs-4546457/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4546457/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjective: \u003c/strong\u003eTo prepare Doxorubicin-loaded Ca-Mg double-doped mesoporous silica nanoparticles (Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX) and examine their properties.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods: \u003c/strong\u003eMesoporous silica nanoparticles (MSNs) and Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e nanoparticles were prepared by hydrothermal method and evaluated for particle size, zeta potential, morphology, and elemental distribution. SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles and Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles were prepared by electrostatic adsorption to investigate their properties, such as particle size, zeta potential, encapsulation efficiency, drug loading, stability, release degree, biosafety, and in vitro antitumor property.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults: \u003c/strong\u003eMSNs and SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles were successfully prepared with a particle size of about 200-300 nm and negatively charged; the MSNs and SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles were regular spherical, and the Ca and Mg doped nanoparticles showed irregular spherical shape, and mapping measured the Ca and Mg elements contains were 2.92% and 1.19% respectively. SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles and Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles were successfully prepared with a particle size of about 300-400 nm, also negatively charged, encapsulation efficiency of about 90%, and a drug loading efficiency of 10%, with a slower release compared to DOX of solution. The release of Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles\u0026nbsp;was faster in an acid condition. All samples’ hemolysis efficiency was less than 5%. Cellular toxicity showed that SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles and Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles are toxic to lower than free DOX. Living and dead cell staining experiments showed similar situations. Cell uptake showed that cell uptake is higher compared with free DOX Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion: \u003c/strong\u003eWe successfully prepared Ca-Mg@SiO\u003csub\u003e2\u003c/sub\u003e@DOX nanoparticles, which have a suitable particle size, zeta potential, and stability, which are released faster in acid conditions, which can reduce the toxicity of DOX, boost cell uptake, and have good antitumor properties.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e","manuscriptTitle":"Preparation of Ca-Mg double-doped mesoporous silica nanoparticles and their drug-loading and drug-releasing properties","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-07-04 18:00:32","doi":"10.21203/rs.3.rs-4546457/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"8a3fae9c-3829-4a76-a0c9-84e6abe917d0","owner":[],"postedDate":"July 4th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-09-29T15:53:10+00:00","versionOfRecord":[],"versionCreatedAt":"2024-07-04 18:00:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4546457","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4546457","identity":"rs-4546457","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}