Characterization and disease control potential of biogenic silver nanoparticles against angular leaf spot of cucurbits

Characterization and disease control potential of biogenic silver nanoparticles against angular leaf spot of cucurbits | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Characterization and disease control potential of biogenic silver nanoparticles against angular leaf spot of cucurbits Vivek Sharma, Sourbah Chauhan, Sneh Sharma This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8625380/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 9 You are reading this latest preprint version Abstract The present study focused on the biogenic formation of nanoparticles using leaf extract of Lantana camara. The phytochemical analysis of leaf extract revealed the presence of several bioactive compounds such as alkaloids, flavonoids, saponins, tannins, resin and oils. The synthesis of biogenic nanoparticles was confirmed through various characterization techniques viz. UV-Vis spectroscopy, FTIR analysis (The Fourier-transform infrared spectroscopy),SEM(Scanning Electron Microscope), EDS (The Energy-dispersive X-ray spectroscopy) and XRD (X-ray diffraction) for their optical properties, functional group, morphological, elemental composition and structural properties, respectively. Further, the antibacterial activity of characterized biogenic nanoparticles revealed inhibition zones of 18.17 ± 0.17,19.33 ± 0.17 and 20.17 ± 0.33 (mm) at various concentrations 150,185 and 200µg/ml respectively against Pseudomonas syringae (MTCC-7620). Additionally, the pathogenicity of the bacterial strain was assessed in the pot experiments on Luffa cylindrica plants (sponge gourd), where the characteristic symptoms appeared following inoculation. The biogenic nanoparticles were foliar applied through both the methods i.e. pre-treatment as well as post-inoculation treatments. It was observed that post-inoculation treatment (75ppm) had a higher reduction in disease severity (37.50%) as compared to pre-treatments (33.33%). Experimentally, 0.5% copper oxychloride as a pre-treatment and 1.5% as a post inoculation-treatment demonstrated 29.17% and 41.67% PDI respectively. These findings underscore the potential of L. camara leaf extract-mediated nanoparticles as effective antibacterial agents, offering new prospects alternatives to conventional bactericides. Lantana camara Angular leaf spot Biogenic synthesis Antibacterial activity Characterization Full Text Additional Declarations No competing interests reported. Table 1 to 5 are available in the Supplementary Files section. Supplementary Files Table.docx Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 24 Feb, 2026 Reviews received at journal 17 Feb, 2026 Reviews received at journal 15 Feb, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers invited by journal 09 Feb, 2026 Editor assigned by journal 31 Jan, 2026 Submission checks completed at journal 30 Jan, 2026 First submitted to journal 30 Jan, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8625380","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":588376948,"identity":"3b0da1fd-728e-42ed-91c8-34b6dda38b59","order_by":0,"name":"Vivek Sharma","email":"","orcid":"","institution":"Dr. YSP university of Horticulture and forestry, Solan","correspondingAuthor":false,"prefix":"","firstName":"Vivek","middleName":"","lastName":"Sharma","suffix":""},{"id":588376949,"identity":"05800cb9-05b0-4416-ad71-7d51bc6762e5","order_by":1,"name":"Sourbah Chauhan","email":"","orcid":"","institution":"Dr. YSP university of Horticulture and forestry, Solan","correspondingAuthor":false,"prefix":"","firstName":"Sourbah","middleName":"","lastName":"Chauhan","suffix":""},{"id":588376950,"identity":"67f95102-6095-40bf-ad43-75d07126344c","order_by":2,"name":"Sneh Sharma","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABCElEQVRIiWNgGAWjYHACNiA+AOPYMDBIIIkToyWNdC2HEVpwAfnZzcce/PhzR9582uEHjD/+nE/sn9188AFDjQ0Dn3QDVi0Gd46lG/a2PTOcczvNgJm37XbijDvHkg0YjqUxsMkcwK5FIsdMgrfhMOMM6QQDZsaG24kNN4AijA2HGdgkErA7bEb+N8k/fw7bz5BO/wB02LnE+YS0MNzIYZPmYTucOEM6x4CBh+1A4gZCWgxupJlJy7YdTgZqKQD6Jdl44420ZIOEY2k8uB2W/EzyzZ/DtkCHbQA6zE523o3kgw8+1NjIyc/A4TAkwP4DSDg2gJhAxTwE1cOAPdEqR8EoGAWjYMQAAPHJXsNZ3HDWAAAAAElFTkSuQmCC","orcid":"","institution":"Dr. YSP university of Horticulture and forestry, Solan","correspondingAuthor":true,"prefix":"","firstName":"Sneh","middleName":"","lastName":"Sharma","suffix":""}],"badges":[],"createdAt":"2026-01-17 10:53:17","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8625380/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8625380/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":102398144,"identity":"85a99d47-ee78-4ce8-8ca2-acf66c0f5c6f","added_by":"auto","created_at":"2026-02-11 10:21:15","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1614317,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8625380/v1_covered_af259a9f-9dbe-431b-a245-20da960530c1.pdf"},{"id":102375647,"identity":"eed831bb-b854-4614-9013-2f9032413f6d","added_by":"auto","created_at":"2026-02-11 05:21:10","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":19329,"visible":true,"origin":"","legend":"","description":"","filename":"Table.docx","url":"https://assets-eu.researchsquare.com/files/rs-8625380/v1/378c4879146df056280c18c5.docx"}],"financialInterests":"\u003cp\u003eNo competing interests reported.\u003c/p\u003e\n\u003cp\u003eTable 1 to 5 are available in the Supplementary Files section.\u003c/p\u003e","formattedTitle":"Characterization and disease control potential of biogenic silver nanoparticles against angular leaf spot of cucurbits","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"discover-plants","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Discover Plants](https://link.springer.com/journal/44372)","snPcode":"44372","submissionUrl":"https://submission.springernature.com/new-submission/44372/3","title":"Discover Plants","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Discover Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Lantana camara, Angular leaf spot, Biogenic synthesis, Antibacterial activity, Characterization","lastPublishedDoi":"10.21203/rs.3.rs-8625380/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8625380/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe present study focused on the biogenic formation of nanoparticles using leaf extract of \u003cem\u003eLantana camara.\u003c/em\u003e The phytochemical analysis of leaf extract revealed the presence of several bioactive compounds such as alkaloids, flavonoids, saponins, tannins, resin and oils. 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