Peer Review #3 of "BMSC-derived exosomal miR-27a-3p and miR-196b-5p regulate bone remodeling in ovariectomized rats (v0.1)"

Guohua Lai, Renli Zhao, Weida Zhuang, Zuoxu Hou, Zefeng Yang, Peipei He, Jiachang Wu, Hongxun Sang, B Abrahamsen, M Skjdt, P Vestergaard, C Chen, P Cheng, H Xie, H Zhou, X Wu, E Liao, X Luo, H Chen, Y Tung, C Chuang, M Tu, T Tsai, S Chang, S Chen, Y Tang, Y Liu, P Zhang, L Lv, X Zhang, L Jia, Y Zhou, G Furesi, A De Jesus Domingues, D Alexopoulou, A Dahl, M Hackl, J Schmidt, S Kalkhof, T Kurth, H Taipaleenmki, S Conrad, C Hofbauer, M Rauner, L Hofbauer, R Kalluri, V Lebleu, D Li, J Liu, B Guo, C Liang, L Dang, C Lu, X He, H Cheung, L Xu, B He, B Liu, A Shaikh, F Li, L Wang, Z Yang, D Au, S Peng, Z Zhang, B Zhang, X Pan, A Qian, P Shang, L Xiao, B Jiang, C Wong, J Xu, Z Bian, Z Liang, D Guo, H Zhu, W Tan, A Lu, G Zhang, W Liu, P Wang, Z Xie, S Wang, M Ma, J Li, M Li, S Cen, S Tang, G Zheng, G Ye, Y Wu, H Shen, D Lizneva, T Yuen, L Sun, S Kim, I Atabiekov, L Munshi, S Epstein, M New, M Zaidi, S Ma, C Tong, E Ibeagha-Awemu, X Zhao, Y Ma, Z Shan, J Ma, Q Wang, J Chu, P Xu, A Qin, Fan, S, S Manolagas, P Marie, M Kassem, F Marini, L Cianferotti, M Brandi, M Mittelbrunn, C Gutirrez-Vzquez, C Villarroya-Beltri, S Gonzlez, F Snchez-Cabo, M Gonzlez, A Bernad, F Snchez-Madrid, A Montecalvo, A Larregina, W Shufesky, D Stolz, M Sullivan, J Karlsson, C Baty, G Gibson, G Erdos, Z Wang, J Milosevic, O Tkacheva, S Divito, R Jordan, J Lyons-Weiler, S Watkins, A Morelli, S Ohno, M Takanashi, K Sudo, S Ueda, A Ishikawa, N Matsuyama, K Fujita, T Mizutani, T Ohgi, T Ochiya, N Gotoh, M Kuroda, F Pang, X Wang, Z Chen, M Zhang, C Wang, X Yang, Q An, L Du, F Wang, G Li, H Huang, B Li, Y Zheng, L Zhang, Wang, F, M Qiu, S Zhai, Q Fu, D Liu, L Ren, R Yao, X Gong, K Yang, K Sheinerman, V Tsivinsky, L Abdullah, F Crawford, S Umansky, T Su, Y Xiao, Q Guo, C Li, Y Huang, Q Deng, J Wen, F Zhou, W Sun, C Zhao, Y Li, G Nie, J Peng, A Wang, W Tian, Q Li, J Song, X Xu, Y Tian, D Zhao, Z Xu, G Zhong, B Han, S Ling, Y Chang, Y Takafuji, K Tatsumi, N Kawao, K Okada, M Muratani, H Kaji, C Thry, S Amigorena, G Raposo, A Clayton, H Valadi, K Ekstrm, A Bossios, M Sjstrand, J Lee, J Ltvall, W Wang, S Qiao, B Sun, J Yang, X Li, S Qian, Y Gu, H Lai, Z Hou, K Lv, G Kan, H Cao, H Wu, Q Sun, M Zhu, X Xie, T Tang, A Hong, Y Bai, F He, Y Wang, X Zhou, D Wang, J Wu, J Sun, Z Zhou, Y Zhang, Q Xu, Y Cui, J Luan, H Li, J Han, R Xu, X Shen, Y Si, Y Fu, W Zhu, T Xiao, Z Fu, J Cheng, H Jiang, Y Xu, Z Zhu, L Li, Y Jin, C Ma, W Zhang, Y Yang, A Chen, W Liang, G Liang, B Huang, Jin, D, L Yu, B Sui, W Fan, L Lei, L Zhou, L Yang, Y Diao, Z Li, X Hao, Z Zeng, L Fei, J Zuo, Z Huang, H Zhang, K, J Zhang, X Yu, Y Yu, Y Gong, H Zhao, X Han, T Zhao, P Qu, X Cao, P Li, Y Fan, X Ma, R Sun, W Li, L Zhong, H Fu, C Lv, X Jia
peer-review OA: gold CC0
AI-generated summary by claude@2026-06, 2026-06-08

BMSC-derived exosomes from ovariectomized rats enhanced osteoclast differentiation, with exosomal miR-27a-3p and miR-196b-5p identified as key regulators of bone remodeling.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Background.In the bone marrow microenvironment of postmenopausal osteoporosis (PMOP), bone marrow mesenchymal stem cell (BMSC)-derived exosomal miRNAs play an important role in bone formation and bone resorption, although the pathogenesis has yet to be clarified.Methods.BMSC-derived exosomes from ovariectomized rats (OVX-Exo) and sham-operated rats (Sham-Exo) were co-cultured with bone marrow-derived macrophages to study their effects on osteoclast differentiation.Next-generation sequencing was utilized to identify the differentially expressed miRNAs (DE-miRNAs) in OVX-Exo and Sham-Exo, while target genes were analyzed using bioinformatics.The regulatory effects of miR-27a-3p and miR-196b-5p on osteogenic differentiation of BMSCs and osteoclast differentiation were verified by gain-of-function and loss-of-function analyses. Results.Osteoclast differentiation was significantly enhanced in the OVX-Exo treatment group compared to the Sham-Exo group.Twenty DE-miRNAs were identified in OVX-Exo and Sham-Exo, among which miR-27a-3p and miR-196b-5p promoted the expressions of osteogenic differentiation markers in BMSCs.In contrast, knockdown of miR-27a-3p and miR-196b-5p increased the expressions of osteoclastic markers in osteoclast.These 20 DE-miRNAs were found to target 11435 mRNAs.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these target genes were involved in several biological processes and osteoporosis-related signaling pathways. Conclusion.BMSC-derived exosomal miR-27a-3p and miR-196b-5p may play a positive regulatory role in bone remodeling.

My notes (saved in your browser only)

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.

References (41)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK