In vitro calcification of bioprosthetic heart valves: test method validation on prosthetic heart valves

In vitro calcification of bioprosthetic heart valves: test method validation on prosthetic heart valves | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article In vitro calcification of bioprosthetic heart valves: test method validation on prosthetic heart valves Nicole Kiesendahl, Christoph Schmitz, Lars Peters, Marek Weiler, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4723288/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A major reason for the failure of bioprosthetic heart valves is calcification. Various pretreatment methods are developed to reduce the calcification behavior. The effectiveness of these methods has so far been investigated in expensive and time-consuming large animal studies. In order to provide a cost-, animal- and possibly also time-saving method, we developed an accelerated dynamic in vitro calcification test method. In the present study, we validated this method using a comparative test of two differently pretreated groups of porcine heart valve bioprostheses (T6 sodium dodecyl sulfate surfactant versus No-T6 treatment). Each group, whose calcification behavior was known from an animal study, contained N = 4 identical surgically implantable aortic bioprostheses. Calcification onsets, progression and extent were detected by high-speed video (HSV) documentation and microscopy. Structural identification of the deposits was carried out by X-ray powder diffraction (XRD). Semidestructive quantification of the calicifications was done by µ-CT as well as destructive chemical quantification of calcium masses per g tissue dry weight via colorimetry and compelxometry. The histomorphologic localization of the calcifications was examined by von Kossa staining. Structural analysis of the investigated deposits indicated “biological apatite” for both test groups. Histological examination revealed sandwich-like localization of the calcifications in the spongiosa zone of the cusps. Quantification of the calcifications showed a distinctly stronger calcification tendency of the No-T6 compared to the anti-calcifying pretreated T6 bioprostheses. We developed and validated a novel and unique test method for in vitro calcification assessment. The quantitative calcification tendencies of the two test groups are comparable with the results of an in vivo study in sheep of these two test groups, after a total of 20 weeks of testing. The structural findings are in line with published in vivo observations and the histomorphological localization appears as known for porcine prostheses. Biomedical Engineering Comparative calcification study HAP quantification via µ-CT complexometric Ca quantification histomorphologic localization of calcifications structural identification Full Text Additional Declarations The authors declare no competing interests. Supplementary Files ESM1.pdf ESM2.pdf ESM3.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4723288","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":325643372,"identity":"cca0ee79-c28d-42a3-b5b1-29c517ee5791","order_by":0,"name":"Nicole Kiesendahl","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Nicole","middleName":"","lastName":"Kiesendahl","suffix":""},{"id":325643373,"identity":"2c540e52-ff8a-496b-9d90-95299f112c8f","order_by":1,"name":"Christoph 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Various pretreatment methods are developed to reduce the calcification behavior. The effectiveness of these methods has so far been investigated in expensive and time-consuming large animal studies. In order to provide a cost-, animal- and possibly also time-saving method, we developed an accelerated dynamic in vitro calcification test method.\u003c/p\u003e\n\u003cp\u003eIn the present study, we validated this method using a comparative test of two differently pretreated groups of porcine heart valve bioprostheses (T6 sodium dodecyl sulfate surfactant versus No-T6 treatment). Each group, whose calcification behavior was known from an animal study, contained N = 4 identical surgically implantable aortic bioprostheses. Calcification onsets, progression and extent were detected by high-speed video (HSV) documentation and microscopy. Structural identification of the deposits was carried out by X-ray powder diffraction (XRD). 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The quantitative calcification tendencies of the two test groups are comparable with the results of an in vivo study in sheep of these two test groups, after a total of 20 weeks of testing. The structural findings are in line with published in vivo observations and the histomorphological localization appears as known for porcine prostheses.\u003c/p\u003e","manuscriptTitle":"In vitro calcification of bioprosthetic heart valves: test method validation on prosthetic heart valves","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-08-02 05:29:42","doi":"10.21203/rs.3.rs-4723288/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"dadc1ecf-22b6-4287-af68-7046d314adc3","owner":[],"postedDate":"August 2nd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":34451160,"name":"Biomedical Engineering"}],"tags":[],"updatedAt":"2024-08-02T05:29:42+00:00","versionOfRecord":[],"versionCreatedAt":"2024-08-02 05:29:42","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4723288","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4723288","identity":"rs-4723288","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}