Topography and Nanomechanics of the Tomato Brown Rugose Fruit Virus Suggest a Fragmentation-Driven Infection Mechanism

Tomato brown rugose fruit virus (ToBRFV) has been causing severe agricultural damages worldwide since its recent discovery. While related to tobacco mosaic virus, its properties and infection mechanisms are poorly understood. To uncover structure and nanomechanics, we carried out atomic force microscopy (AFM) measurements on individual ToBRFV particles. Virions are rod-shaped with a height and width of 9 and 30 nm, respectively. Length is widely distributed (5-1000 nm) with a mode at 30 nm. ToBRFV rods displayed a 22.4-nm axial periodicity related to structural units. Force spectroscopy revealed a Young modulus of 8.7 MPa, a spring constant of 0.25 N/m, and a rupture force of 1.7 nN. In the force curves a step was seen at a height of 3.3 nm, which is related to virion wall thickness. Wall thickness was also estimated by predicting coat-protein structure with AlphaFold, yielding a protein with a length of 7.3 nm. Accordingly, the structural element of ToBRFv is a right circular cylinder with equal height and diameter of ~22 nm, and a wall thickness between 3.3-7.3 nm. Thus, at least 4-9 serially-linked units are required to encapsidate a single, helically organized RNA genome. Fragmentation of ToBRFV into these cylindrical structural units may result in a facilitated release of the genome and thus efficient infection.