Characterisation of the Pathogenicity of Beauveria sp. and Metarhizium sp. Fungi Against Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)

Previously, we assessed the pathogenicity of eleven endemic entomopathogenic fungi (EPF) including six Beauveria isolates, four Metarhizium isolates, and one M. pingshaense, against the agricultural pest Spodoptera frugiperda (fall armyworm, FAW). We found that four Beauveria and one Metarhizium isolates were effective, with Beauveria isolates B-0571 and B-1311 exhibited high mortality within 24 hours post spore application. This study aimed to identify and characterise entomopathogenesis mechanisms of these isolates as potential FAW biocontrol agents. All Beauveria isolates were determined as B. bassiana, the Metarhizium isolates as two M. robertsii, one M. majus, and an unknown Metarhizium species. Despite the high mortality from B-0571 and B-1311 isolates, scanning electron microscopy showed no fungal spore germination on dead larvae 24 hours after spore application. Four insecticide compound gene clusters, i.e., bassianolide, beauvericin, beauveriolide, and oosporein, were identified and characterised in all B. bassiana isolates. These compounds are hypothesised to contribute to the high early mortality rates in FAW. Identifying and characterising of gene clusters encoding these insecticide compounds in B-0571 and B-1311 will contribute to better understanding the entomopathogenicity of these isolates that will be vital to developing these EPF isolates as sustainable alternative FAW biocontrol agents.