Establishment of Intestinal Organoids from Common Marmosets

Intestinal organoids are useful for in vitro investigation of the properties of intestinal epithelial cells and their interaction with gut microbiome. Here, we established cecal and colonic organoids from common marmosets, which are highlighted as model nonhuman primates but susceptible to gastrointestinal diseases. Established organoids were capable of passaging and long term culture. Results of quantitative reverse transcription PCR and immunostaining showed differentiation of the organoids into major cell types in the intestinal epithelium (colonocytes, goblet cells and enteroendocrine cells), enabling in vitro analysis of these cells in marmosets. The organoids will be a useful model for the investigation of gut physiology related to gastrointestinal diseases and gut-microbiome interactions, further expanding medical, biological and veterinary research in the future.