Inositol pyrophosphate profiling reveals regulatory roles of IP6K2-dependent enhanced IP7 metabolism in enteric nervous system

Inositol pyrophosphates (PP-IPs) regulate diverse physiological processes; to better understand their functional roles, assessing their tissue-specific distribution is important. Here, we profiled PP-IP levels in mammalian organs using a novel HILIC-MS/MS protocol and discovered that the gastrointestinal tract (GIT) contained the highest levels of IP 7 and its precursor IP 6 . Although their absolute levels in the GIT is diet-dependent, elevated IP 7 metabolism still exists under dietary regimes devoid of exogenous IP 7 . Of the major GIT cells, enteric neurons selectively express the IP 7 -synthesizing enzyme IP6K2. IP6K2 -knockout mice exhibited significantly impaired IP 7 metabolism in the various organs including the proximal GIT. Additionally, HILIC-MS/MS analysis displayed that genetic ablation of IP6K2 significantly impaired IP 7 metabolism in the gut and duodenal muscularis externa containing myenteric plexus. Whole transcriptome analysis of duodenal muscularis externa further suggested that IP6K2 inhibition induced the gene sets associated with mature neurons such as inhibitory, GABAergic and dopaminergic neurons, concomitantly with suppression of those for neural progenitor/stem cells and glial cells. In addition, IP6K2 inhibition explicitly affected transcript levels of certain genes modulating neuronal differentiation and functioning, implying critical roles of IP6K2-IP 7 axis in developmental and functional regulation of enteric nervous system. These results collectively reveal an unexpected role of mammalian IP 7 —a highly active IP6K2-IP 7 pathway is conducive to enteric nervous system.