Attenuated viral strains of priority pathogens for potential use in controlled human infection model studies: A scoping review

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Hamilton , V. Simpson , T. Fox , V. Lutje , A. Kohl , D. M. Ferreira , B. Morton doi: https://doi.org/10.1101/2025.07.04.25329749 D.O. Hamilton 1 Department of Clinical Sciences, Liverpool School Tropical Medicine , Liverpool, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for D.O. Hamilton For correspondence: oliver.hamilton{at}lstmed.ac.uk V. Simpson 1 Department of Clinical Sciences, Liverpool School Tropical Medicine , Liverpool, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site T. Fox 1 Department of Clinical Sciences, Liverpool School Tropical Medicine , Liverpool, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site V. Lutje 1 Department of Clinical Sciences, Liverpool School Tropical Medicine , Liverpool, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site A. Kohl 2 Centre for Neglected Tropical Diseases, Departments of Tropical Disease Biology and Vector Biology, Liverpool School of Tropical Medicine , Liverpool, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site D. M. Ferreira 1 Department of Clinical Sciences, Liverpool School Tropical Medicine , Liverpool, UK 3 Oxford Vaccine Group, Department of Paediatrics, University of Oxford , Oxford, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site B. Morton 1 Department of Clinical Sciences, Liverpool School Tropical Medicine , Liverpool, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site Abstract Full Text Info/History Metrics Supplementary material Data/Code Preview PDF Abstract Background There are several known pathogens and families identified as high risk for pandemic potential. It is essential to study these pathogens and develop medical countermeasures to mitigate disease prior to potential pandemics. Controlled human infection models (CHIMs) using attenuated viral strains may offer an efficient and safe way to do this. Objective Our aim was to systematically examine the literature for attenuated, but replication competent, strains of Coalition for Epidemic Preparedness Innovations (CEPI) identified priority pathogens (Ebola, Lassa, Nipah, Rift Valley fever, chikungunya and Middle East respiratory syndrome) that have been administered to humans. Design A comprehensive literature search of multiple databases was performed by an information specialist. All search results were screened by two authors against inclusion/exclusion criteria from a pre-specified protocol. The primary outcome was confirmation that the administered viral strain could subsequently be recovered from participants. The secondary outcome was attenuated virus safety. Results Our searches yielded 13078 results and 5998 articles remained for screening after removing duplicates and animal studies. Subsequently, 351 articles were selected for full text review and nine were included for data extraction. Four distinct attenuated strains were identified across two priority pathogens – TSI-GSD-218 and VLA1553 for chikungunya and MP-12 and hRVFV-4s for Rift Valley Fever. Attenuated virus was recovered for each strain except hRVFV-4s. There were no major safety concerns for these identified strains. Conclusions We have identified three attenuated viral strains that may be amenable to development into novel CHIMs for two priority pathogens. Of these, VLA1553 for chikungunya is in the most advanced stage of development. There is a research gap for the creation of new attenuated mutants that could be utilised in CHIM for other priority pathogens. Author summary There are several families of viruses that scientists predict are most likely to cause a future pandemic, such as coronaviruses or Ebola. Studying these viruses ahead of time might mean we have vaccines or drugs available ahead of time. Human challenge studies involve exposing healthy volunteers to germs that might make them sick. It wouldn’t be safe to do that for many illnesses that could cause a pandemic. However, it may be possible to use weakened versions of these viruses instead. We have performed a thorough search of scientific papers to look for candidates of weakened viruses to see if we could use them like this. The most promising is a version of the Chikungunya virus that is currently used as a vaccine. 1. Introduction Viruses with epidemic and pandemic potential risk destabilising international economies and social order; and could cause mass illness and deaths ( 1 ). This threat is increased by anthropogenic climate change; land-use ecosystem changes, increased human and livestock populations; and potentially due to bioterrorism ( 2 ). The Coalition for Epidemic Preparedness Innovations (CEPI) have targeted the manufacture of safe vaccines, therapeutics, and diagnostics within 100 days of identification of an emerging pandemic ( 3 ). Modelling data suggests that over 8 million deaths and $14 trillion could have been saved if this 100-day target had been met during the COVID-19 pandemic ( 4 ). Whilst the causative organism of the next viral pandemic may be entirely novel (so called “Disease X” ( 2 )), there are several known pathogens or families identified as high risk for pandemic potential, particularly coronaviridae , orthomyxoviridae and filoviridae ( 1 , 5 – 7 ). CEPI have defined nine diseases prioritised for development of medical countermeasures (MCMs) prior to a potential pandemic: Ebola (EBOV); Lassa (LV); Mpox; Nipah (NiV); Rift Valley fever (RVFV); chikungunya (CHIKV); COVID-19; Middle East respiratory syndrome (MERS); and “Disease X” ( 5 , 8 ). Development of MCMs against these viruses is essential to improve pandemic preparedness ( 1 , 5 ). As occurred in the COVID-19 pandemic, knowledge gained from studying one of these viruses may also expedite MCM development if a novel pandemic virus were to emerge from a related family or genus ( 9 , 10 ). Standard Phase 1-3 efficacy studies to develop experimental MCMs may be impossible due to limited or absent participants to enrol into trials prior to disease outbreaks. Controlled human infection models (CHIMs) may offer an efficient solution to this critical dilemma ( 11 ). Traditionally, CHIMs involve deliberate exposure of an infectious dose of a pathogen to carefully selected volunteers ( 12 ). CHIMs may significantly accelerate the development of MCMs in a safe, efficient and cost-effective way ( 13 – 15 ), for example by up- or down-selecting potential agents to ensure that only the most promising candidates are progressed to pivotal efficacy trials ( 14 , 16 ). CHIM derived data has recently been used for regulatory approvals for VaxChora for travellers ( 17 ) and the World Health Organization (WHO) pre-qualification of the use of a typhoid conjugate vaccine (TypbarTCV) in endemic regions ( 18 , 19 ). It is recognised that CHIMs may support the emergency use of an investigational vaccine in a pandemic scenario ( 20 ) and therefore could be a crucial tool in pandemic preparedness ( 4 , 16 ). Given the inherent risks from some of these priority pathogens, CHIMs that incorporate wild type viral infection would be unethical ( 21 – 23 ) and therefore novel approaches to study design are required. Previous studies have used attenuated versions of a disease-causing pathogen to mitigate these risks ( 14 , 21 , 24 ), usually repurposing an attenuated strain designed as a live-attenuated vaccine. This is sometimes termed ‘pseudochallenge’ ( 14 ). The approach has been successful in CHIM with several other diseases, most notably dengue virus ( 25 , 26 ), as well as tuberculosis ( 27 ), influenza ( 28 ), rotavirus, and poliovirus ( 14 ). Given the lack of precedent with CHIMs in these diseases and the presumed heterogeneity of studies, we have conducted a scoping review to systematically examine the literature for attenuated strains of CEPI priority pathogens that have already been administered to humans. This will identify candidates that may be developed into novel CHIMs to facilitate trials for MCMs. 2. Methods The objectives, eligibility criteria and methods for this scoping review were specified in advance and published in a prospectively registered protocol on the Open Science Framework ( https://osf.io/nu3bf/ ). The scoping review was conducted according to methodology from the JBI Manual for Evidence Synthesis ( 29 ) and incorporates the PRISMA Extension for Scoping Reviews (PRISMA-ScR) checklist ( 30 ) (see supplementary appendix S1). 2.1 Search strategy and eligibility criteria A comprehensive literature search was last performed on 24 th February 2025 in the Cochrane Central Register of Controlled Trials (CENTRAL, published in the Cochrane Library), MEDLINE (via OVID), Embase (via OVID), Science Citation Index (Web of Science), CAB Abstracts & Global Health (Web of Science) databases. We also searched the WHO International Clinical Trials Registry Platform (ICTRP; apps.who.int/trialsearch/) and ClinicalTrials.gov ( https://clinicaltrials.gov/ct2/home ) for trials in progress. The full search terms used are included in the supplementary appendix S2. There were no restrictions on language, region, date, participant demographics, or publication status. Additionally, references of all identified reviews were also hand-searched to identify potential additional eligible studies. 2.2 Study selection Inclusion criteria were determined a priori : adult humans (≥ 18 years old); deliberately exposed to a near-whole-genome, attenuated version of any of the following priority viruses: Ebolavirus; Lassa mammarenavirus; Nipah virus; Rift Valley fever phlebovirus; chikungunya virus; or Middle East respiratory syndrome–related coronavirus (CoV). For a successful CHIM, it is essential to confirm infection and clearance with microbiological or virological techniques ( 27 , 31 ). Therefore, we excluded studies of mutations that preclude representative viral replication: chemical inactivation; irradiation; sub-unit vaccines; virus-like particles; recombinant viruses with genetic material from another virus such as vesicular stomatitis virus or chimpanzee adenovirus; and mRNA or DNA vaccines. Studies without primary data, such as editorials or systematic reviews were also excluded. COVID-19 has already been developed into a well-established CHIM ( 32 , 33 ) and was therefore not included in this scoping review. CEPI lists a hypothetical, unknown “Disease X” as a priority pathogen for vaccine development. An unknown disease is not amenable to human challenge so this priority pathogen group was excluded. Some strategies for the rapid development of a CHIM for a new pathogen in a pandemic scenario are discussed elsewhere and are outside the scope of this review ( 16 , 34 – 36 ). Mpox was designated a CEPI Priority Pathogen online after completion of the scoping review protocol and was not included ( 8 ). Two investigators (DOH and VS) independently screened titles and abstracts using Rayyan ( https://www.rayyan.ai/ ) ( 37 ). The first 25 title and abstracts were screened together as a pilot to ensure consistency. No automated tools were used. All abstracts deemed potentially eligible by either author proceeded for full text review by both authors, recorded using Microsoft Excel (Microsoft, WA, US). Discrepancies of full text studies were resolved by discussion or by a third investigator (BM). 2.3 Data extraction The primary outcome was confirmation the administered viral strain could subsequently be recovered from participants. The secondary outcome was safety of the mutant viruses. Other outcomes were narratively summarised where reported, namely: author; year; institution; mutation from wild-type; study phase; dosage; sample size; comparator; adverse events (AE)/serious adverse events (SAE); follow-up length; and availability and regulatory requirements. The full framework for data extraction is presented in Supplementary Table S2. This was developed iteratively with input from authors expert in CHIM development as the search developed. Data was extracted by a single-author (DOH), recorded using Microsoft Excel, and checked by a second (VS). A risk of bias assessment was conducted by a single author (DOH). We used the original Cochrane Collaboration Tool ( 38 ) for randomised studies. This tool was the most appropriate because the outcome of interest in our review (viraemia) was not the primary outcome of the studies evaluated and this tool provides a general risk of bias assessment rather than against a particular outcome. Non-randomised studies were assessed using the ROBINS-E tool ( 39 ). 2.4 Data synthesis Some attenuated strains were investigated in more than one study. In those cases, the methodology and results of those studies are presented together – see Table 1 and Supplementary Table S2. We have provided a descriptive and quantitative (where appropriate) summary for each identified attenuated strain. A background for each pathogen is also presented prior to the description of any identified attenuated strains. View this table: View inline View popup Table 1: Studies included after full-text review 3. Results The literature search resulted in 13,078 studies (n=6242 for EBOV, n=2517 for MERS, n=1591 for CHIKV, n=1197 for RVFV, n=911 for LV and n=620 for NiV). We first removed 3653 duplicates and then, as per our protocol, removed 3427 articles found via search-terms that referenced non-human primates, with the option they could be included later if very limited human data was found (this step was not subsequently required). Thus, 5998 studies remained for title and abstract screening. Of these, 351 manuscripts were selected for full text review and nine were included for data extraction (see Figure 1 ). Table 1 and Supplementary Table S2 describe the characteristics of included studies and each pathogen is described separately. Table 2 presents the pipeline of attenuated viruses across the pathogens. All attenuated viruses identified in this scoping review had been developed as part of a search for an effective live-attenuated vaccine. Download figure Open in new tab Figure 1 PRISMA Flow Diagram for the Scoping Review process CHIKV = chikungunya, EBOV = Ebola, LV = Lassa, MERS = Middle East Respiratory Syndrome, NiV = Nipah Virus, RVFV = Rift Valley Fever View this table: View inline View popup Download powerpoint Table 2: Pipeline of attenuated virus controlled human infection model candidates for Coalition for Epidemic Preparedness Innovations (CEPI) identified priority pathogens (excluding COVID, mpox and “disease X”) across the translational development pipeline, organised by strains in most advanced stage of study. Of the nine included studies, five were randomised controlled trials ( 40 – 44 ), two were randomised controlled Phase 1 trials with a non-randomised safety or confirmatory cohort ( 45 , 46 ) and two were non-randomised interventional studies ( 47 , 48 ). Five of the nine studies investigated for recovery of attenuated virus ( 40 , 44 – 47 ). Figure 2 presents risk of bias assessments for the included studies. Only one study was found to be at high risk of bias ( 45 ). Download figure Open in new tab Figure 2: Risk of bias assessment performed on the included studies (Cochrane Collaboration tool for randomised studies ( 38 ) and ROBINS-E tool for non-randomised studies ( 39 )). C1 – random sequence generation (selection bias), C2 – allocation concealment (selection bias), C3 – blinding of participants and personnel (performance bias), C4 – blinding of outcome assessment (detection bias), C5-incomplete outcome data (attrition bias), C6 – selective reporting (reporting bias), C7 – other bias. D1 – risk of bias due to confounding, D2 – risk of bias arising from measurement of the exposure, D3 – risk of bias in selection of participants into the study, D4 – risk of bias due to post-exposure interventions, D5 – risk of bias due to missing data, D6 – risk of bias arising from measurement of the outcome, D7 – risk of bias in selection of the reported result. 3.1 CHIKV This alphavirus is transmitted by Aedes mosquitoes and can cause explosive epidemics, particularly in urban areas ( 23 , 60 , 61 ). At the time of writing, there is an active epidemic in La Réunion with >47,000 cases reported since August 2024 ( 62 ). CHIKV is endemic to several continents including Africa, Asia, the Americas and, more recently, southern Europe ( 63 , 64 ). There is international concern that climate change will increase the spread of CHIKV by expanding the habitat for its vector into previously infection-naïve populations ( 63 , 64 ). Chikungunya disease is characterised by fever, malaise and arthralgia with a case-fatality rate around 0.1%, although this can be higher in older or co-morbid adults ( 23 ). The disease is associated with a high level of long-term morbidity as it can lead to a chronic, debilitating arthritis, which accounts for a substantial global socioeconomic burden ( 65 ). There are no licenced antiviral treatments against this infection ( 66 ). There are two recently licensed vaccines against CHIKV: Ixchiq, a live-attenuated vaccine discussed in detail below ( 67 ); and Vimkunya, a virus-like particle (VLP) vaccine ( 68 , 69 ). There are multiple attenuated CHIKV strains that have been used in pre-clinical or animal models ( 70 – 79 ), although only two have proceeded to use in humans, VLA1553 and TSI-GSD-218. 3.1.1 VLA1553 VLA1553 (initially termed ‘Δ5nsP3’), was developed as a single-shot live-attenuated vaccine. First described by Hallengärd et al ( 80 ), attenuation was achieved by deleting part of the gene nsP3 encoding the non-structural replicase complex protein nsP to eliminate the risk of reversion. The candidate is based on the 2004-2005 epidemic La Réunion strain, produced in Vero cells and purified by centrifugation, ultrafiltration, batch-chromatography, and sucrose gradient centrifugation. The mutated virus was shown to be genetically stable, safe and protective in a mouse model ( 80 ), and later in a non-human primate model ( 81 ). Wressnigg et al. conducted a Phase 1 study in 120 healthy adults using three different doses of VLA1553 ( 46 ). Systemic solicited AEs were experienced by 52.5% (63/120) of participants and 10.8% (13/120) of participants experienced severe related adverse events. There were no adverse events of special interests (AESIs) and there was one unrelated SAE (Supplementary Table S2). All cohorts experienced a transient viraemia after the first dose, detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR) using a hydrolysis probe and primers specific to the CHIKV gene nsP1. This viraemia peaked at Day 3 and resolved by Day 14. Shedding of attenuated viruses was only detected at one time point in one participant. Of note, in 94 participants administered repeat immunisation 6-12 months later, viraemia was not detectable and only 3.2% (3/94) of participants experienced targeted systemic symptoms solicited by investigators. The safety of the candidate was demonstrated in a large double-blind, multicentre, placebo-controlled, randomised Phase 3 trial when given to 3093 participants ( 42 ). AEs were experienced by 62.5% (1926/3082) of participants who received VLA1553, with the majority headache, fatigue and myalgia, with 18.0% (554/3082) of the experienced arthralgia. SAEs were reported in 1.5% (46/3082) of participants exposed to VLA1553 compared to 0·8% (8/1033) of participants in the placebo arm, although only two of the SAEs were deemed related to the vaccine. Viraemia was not reported in this study. A second Phase 3 study of 408 participants examining three lots of VLA1553 further confirmed the safety of this attenuated virus, although this study did also not investigate for attenuated viraemia ( 43 ). The use of VLA1153 as a live-attenuated vaccine (licenced as ‘Ixchiq’) has been approved in the USA, Canada, the European Union and the UK ( 67 , 82 , 83 ). However, use has recently been paused in adults aged over 60 due to concern regarding post-marketing safety reports in this subpopulation ( 84 ). 3.1.2 TSI-GSD-218 An attenuated version of CHIKV, later termed TSI-GSD-218 and also known as CHIK 181/clone 25, was created by Levitt et al. in 1985 from a Thai strain serially passaged in primary green monkey kidney cells and later in Medical Research Council (MRC)-5 cells in an attempt to develop a live-attenuated vaccine ( 85 ). The attenuation is mediated by two point mutations in the E2 glycoprotein ( 86 ). Phase 1 studies were conducted by the US Military but have been only partially published ( 40 , 48 ). In the cohort of alphavirus-naïve participants a Phase 1 study ( 40 ), 36.8% (14/38) had detectable viraemia upon amplification in cell culture for 1-2 days from Day 4-8, although none could be directly plaqued from serum. The overall number of AEs is not reported although there was a low number of typical solicited AEs and the authors state that these were not significantly different from the placebo cohort. Summary data from unpublished Phase 1 studies are presented by Hoke et al. with no significant safety concerns reported ( 48 ). In a Phase 2 study, 73 healthy volunteers were recruited and randomised 4:1 to TSI-GSD-218 ( 41 ). Related AEs were similar across both cohorts (32% [19/59] vs 29% [4/14]) with two severe related AEs and 8% (5/59) experiencing temporary arthralgia compared to 0% in the placebo arm. The levels of viraemia are not reported in this study. Our search did not identify any studies involving TSI-GSD-218 that were actively recruiting since the year 2000, reportedly due to “changes in assessment of threats to military operations” alongside “anticipated difficulties” in demonstrating efficacy ( 48 ). 3.2 RVFV This bunyavirus is transmitted by multiple mosquito species between humans and ruminants ( 87 ). The resultant RVF disease is a major One Health and economic threat as it can cause epidemics of fatal disease in both humans and livestock ( 88 ). RVFV is endemic to sub-Saharan Africa and the Arabian Peninsula. There is concern it could spread further due to spill-over events from imported infected herds or via the increasing global reach of its vectors ( 89 ). RVFV causes a wide-spectrum of human disease including encephalitis, hepatitis, retinitis and viral haemorrhagic fever, fatal in around 20% of hospitalised patients ( 88 , 90 , 91 ). There are currently no licenced vaccines or antivirals against RVFV for use in humans. There are multiple attenuated versions of RVF in the scientific literature, often developed as a live-attenuated vaccine for animals ( 92 – 104 ), and based on our search, we identified two that have progressed to use in humans ( 44 , 45 , 47 ), strains MP-12 and hRVFV-4s. 3.2.1 MP-12 First developed in the 1980s by the US military, MP-12 is an attenuated strain of RVFV created for both human and veterinary use by performing 12 serial plaque passages of the Egyptian strain ZH548 through MRC-5 cells in the presence of 5-fluorouracil ( 105 ). The attenuation is based on mutations in all of the S-, M- and L-segments of the virus ( 87 ), providing some protection against reversion to wild-type ( 100 ). MP-12 has been shown to be generally safe and immunogenic in ruminants ( 106 – 109 ) and non-human primates ( 110 , 111 ), whilst also causing a low level viraemia. However, it was shown to be potentially teratogenic in early pregnancy and may cause a hepatitis in young animals ( 90 , 99 ). Two Phase 1 studies of MP-12 are unpublished but summarised by Pittman et al. in their Phase 1 dose escalation and route comparison study as part of the assessment of MP-12 as a live-attenuated vaccine ( 45 ). Firstly, four participants received undiluted MP-12 (10 4.4 plaque forming units [PFU]) as a subcutaneous (SC) injection. All four participants developed a mild-moderate transaminitis which resolved without sequelae. Attenuated virus was recovered from one participant using nucleic acid amplification. A further 22 participants were randomised to placebo or four different dilutions of MP-12 SC (Supplementary Table S2). Transaminitis, raised lactate dehydrogenase and creatinine kinase (CK) are reported but the authors summarise the vaccine as “remarkably safe” ( 45 ). In the published data of the Phase 1 study, performed in 1996 and published in 2016, 56 healthy volunteers were randomised to various doses of MP-12 either SC or intramuscular (IM) (Supplementary Table S2) ( 45 ). No SAEs and no significant solicited symptoms are reported, however there was one self-resolving Grade 4 transaminitis. Three Grade 4 rises in CK are also reported, although the authors comment that these were likely related to military exercises performed by participants. Virus could be recovered by direct plating of serum in one participant and a further six by tissue culture amplification and in situ detection via Enzyme-Linked Immunosorbent Assay (ELISA) (7/43 [16.3%] exposed participants). A Phase 2 study by the same group administered 10 5 PFU IM to 19 healthy volunteers ( 47 ). Solicited AEs, including headache, fever and injection site pain, were frequent but well tolerated (Supplementary Table S2). No significant related biochemical abnormalities and no SAEs are reported. Assessment of viraemia was performed on plasma and buffy coat specimens collected daily for 14 days post exposure using both plaque assay and blind, double passage on Vero cells. No viraemia was detected by direct plaque assay in any participant. Viraemia detection using blind, double passage on Vero cells was detectable in 5/19 (26.3%) of participants (1-4 isolates per subject, between Day 4 and Day 9). There was no reversion to wild-type in recovered virus between participants. We identified no studies that administered MP-12 to humans after 2008. There are reports that MP-12 vaccine candidate was paused due to cold-chain and BioSafety Level-3 requirements; and liver toxicity concerns ( 112 ). 3.2.2 hRVFV-4s hRVFV-4s is a mutant RVF virus created by splitting the glycoprotein precursor gene to produce a four-segment virus ( 113 ). It has been demonstrated to be safe in mice ( 113 ), ruminants ( 114 ) and non-human primates ( 115 ), whilst causing no detectable viraemia. A Phase 1 study of 75 participants in a placebo-controlled (3:1), dose-escalation study of hRVFV-4s demonstrated that the attenuated virus was well tolerated with only mild-moderate solicited symptoms and no related Grade 3-4 symptoms or SAEs ( 44 ). Importantly, no vaccine viral RNA was detected via RT-qPCR in any blood, urine, saliva or semen samples from participants at numerous timepoints (days 0, 1, 3, 7, 14, 28, and 180). 3.3 Ebolavirus species Viruses within the Filoviridae are RNA viruses which cause viral haemorrhagic fever (VHF) with high morbidity and mortality and is transmitted person-to-person via direct contact with infected bodily fluids ( 116 ). It has caused devastating epidemics across West and Central Africa, most notably the 2013-2016 West African epidemic which caused 11,325 deaths ( 117 ). There are four species of the genus Ebolavirus that cause disease in humans: Sudan ebolavirus (SUDV), Bundibugyo ebolavirus, Taï Forest ebolavirus (TAFV), and Zaire ebolavirus ( 118 ). The Sudan and Zaire species have historically caused most epidemics ( 117 ). Two vaccines have been licenced in the USA or Europe: rVSVΔG-ZEBOV-GP (Ervebo) ( 119 ) and Ad26.ZEBOV + heterologous MVA-BN-Filo boost (Zabdeno/Mvabea) ( 118 ). Only Mvabea may provide coverage against non-Zaire species as it expresses Zaire, SUDV & Marburg virus glycoproteins plus TAFV nucleoprotein ( 120 ). There are no licenced antiviral medications for EBOV ( 117 ). Attenuated whole-genome EBOV is uncommon in the pre-clinical literature ( 116 , 121 – 125 ), although one, EBOVΔVP30, has progressed to both non-human primate ( 126 ) and Phase 1 human study ( 51 ). 3.3.1 EBOVΔVP30 Halfmann et al. developed a mutant that lacks the viral protein (VP) 30 gene, known as EBOVΔVP30 ( 116 ). VP30 is an essential transcription factor for EBOV and hence this virus is replication-deficient outside of Vero cells expressing VP30 in trans . It has been shown to be genetically stable, morphologically indistinct from wild-type and safe in a mouse model ( 123 ). The mutant was further inactivated with hydrogen peroxide when transferred to a non-human primate model ( 126 ). According to the trial registry, in 2019, a Japanese group enrolled 15-30 healthy human volunteers to a Phase 1 study using EBOVΔVP30 (named ‘iEvac-Z’) ( 51 ). This study remains unpublished, although a conference abstract describes “a strong safety profile in humans” ( 127 ). As there were no published data to assess, the study did not meet our pre-specified inclusion criteria for this scoping review. There are press reports that state a second study was due to open in Sierra Leone in 2024 ( 128 ). 3.4 LV LV is an arenavirus endemic to West Africa ( 129 ). It causes Lassa fever, a VHF responsible for around 5000 deaths per year ( 130 ), for which there are no licenced vaccines or therapeutics. LV may be transmitted by rodents or person-to-person via infected bodily fluids ( 49 ). We identified no example of whole-genome attenuated LV that has been administered to non-human primates or humans. There are however recent examples of live attenuated viruses created by reverse genetics and administered to guinea pigs in an attempt to create a novel live-attenuated vaccine ( 49 , 50 ). The fact that Argentine haemorrhagic fever virus, another arenavirus causing VHF, has a licenced live-attenuated vaccine in current use ( 131 ) gives further credence to the idea that a safe attenuated LV mutant may be feasible. There is an example of an attenuated virus, ML29, rationally created by reassortment of LV and Mopeia virus (MOPV), an attenuated relative of LV, that has been administered to non-human primates ( 132 , 133 ). This carries the replication machinery of MOPV and expresses major antigens of LV, however as this is genetically distinct from LV itself and only results in a low, transient viraemia, it did not meet pre-specified criteria for inclusion in our scoping review ( 133 ). 3.5 NiV NiV is a henipavirus that has caused sporadic outbreaks throughout Asia. It is spread by bats, livestock or human-to-human transmission and therefore of high pandemic potential ( 134 , 135 ). It can cause a lethal encephalitis with a high case-fatality rate and has no licenced therapy or vaccination ( 135 , 136 ). Whilst there are attenuated whole-genome versions of NiV in a pre-clinical setting ( 58 , 59 ), we identified no studies that have progressed to human use. 3.6 MERS-CoV MERS-CoV is a coronavirus similar to severe acute respiratory syndrome coronavirus (SARS-CoV) and COVID-19, which can cause a fatal pneumonia with a high case-fatality rate ( 137 ). It is spread by dromedary camels or by person-to-person contact ( 138 ). MERS is largely contained within in the Arabian Peninsula, although it has caused a large outbreak in South Korea when imported by a returning traveller ( 139 ). There are no current licenced vaccines or antivirals for MERS ( 140 ). Whilst there are multiple attenuated whole-genome versions of MERS-CoV in pre-clinical use ( 52 – 57 ), we identified no studies that have progressed to human use. 4. Discussion We have conducted a rigorous scoping review to identify attenuated strains of six CEPI priority pathogens that have been used in humans that could potentially be progressed into novel human challenge models. We have identified four such strains across two priority pathogens, although only three strains (TSI-GSD-218 ( 40 , 41 , 48 ) and VLA1553 ( 42 , 43 , 46 ) for CHIKV and MP-12 ( 45 , 47 ) for RVFV) that produce the required virological response necessary for a CHIM ( 27 ). The final identified strain, hRVFV-4s of RVFV, does not cause viraemia in pre-clinical settings ( 115 ) nor was viraemia detected in humans despite robust RT-qPCR testing ( 44 ). There was insufficient published data to assess the attenuated EBOV strain EBOVΔVP30, although based on the pre-clinical data ( 126 ), it is unlikely that it would cause detectable viraemia for use as a primary endpoint within a CHIM. Three of the CEPI priority pathogens, NiV, MERS and LV, have no attenuated strains administered to humans. Of the three identified strains that produce an attenuated viraemia, only VLA1553 has progressed to Phase 3 testing and licensure in the form of the live-attenuated vaccine ‘Ixchiq’ ( 42 , 43 , 67 , 83 ). VLA1553 has been demonstrated to be tolerable and safe and appears to produce a consistent viraemia ( 46 , 83 , 141 ). The lack of viraemia and markedly reduced solicited adverse events seen following re-exposure 6-12 months later can be interpreted as a proof-of-concept that VLA1553 could be useful as a challenge agent investigating other MCMs against CHIKV with viraemia and symptoms as outcome measures. It is unknown if this viraemia would also be detectable if VLA1553 was administered SC or intra-dermally, which would imitate a mosquito bite more closely. Typically, live-attenuated viruses are developed as early vaccine candidates and have a long history of safe use, for example, in yellow fever, smallpox and polio ( 142 ). However, such candidates may be discontinued as potential vaccines if they are unacceptably reactogenic, even if they are safe and immunogenic. Reactogenicity is of lower concern in a human challenge agent if meticulous informed consent is obtained; symptoms are mild/moderate and participants are closely monitored. There is precedent with dengue for progressing an abandoned live-attenuated vaccine candidate (rDEN2Δ30) into a successful attenuated CHIM ( 25 , 143 ). The efficacy of the TV003 vaccine that was demonstrated in the CHIM by Kirkpatrick et al. ( 25 ) was later replicated in Phase 3 field trials ( 144 ). We postulate that this model could be imitated with VLA1553 in CHIKV to test novel therapeutics or additional vaccine candidates that may be more suitable for pregnant, older or immunocompromised patients than VLA1553. A safe CHIM for CHIKV would be an important advance due to: the limitations of animal models ( 145 ); the lack of a universally-accepted correlate of protection ( 23 , 146 ); the difficulties in conducting field tests due to unpredictable and often short-lived outbreaks ( 23 , 66 ); and the lack of licenced antiviral ( 23 , 66 ). Similarly, it may be possible to develop MP-12 into a CHIM for RVFV. It has been shown to be well tolerated and safe in Phase 1 and 2 studies when administered both SC and IM to around 100 healthy volunteers ( 45 , 47 ) and is conditionally licenced in animals ( 112 ). Whilst we identified no human studies that were actively recruiting since 2008, there is suggestion in the literature that this was still being developed as a live-attenuated vaccine in 2020 by the Sabin Vaccine Institute ( 100 ). In order to progress MP-12 into a CHIM, a method of recovering the strain by RT-qPCR would need to be developed. There are a number of other attenuated RVFV strains used in veterinary practice but none appear suitable for development into CHIM, either due to safety concerns in the case of the Smithburn strain ( 87 ) or the lack of viraemia in the Clone-13 strain ( 100 ). There are also several so-called “next-generation” MP-12 strains in pre-clinical development, such as r2segMP12, DDVax and RVax-1 ( 87 , 99 ). These are strains that have been attenuated through reverse genetics of the NSs protein, a major virulence factor, however their safety in humans or their ability to cause a viraemia is currently unknown ( 87 ) and further study would be required before incorporation into a CHIM. During the COVID-19 pandemic, SARS-CoV-2 CHIM studies were performed ( 32 , 33 ) after development of a robust ethical framework ( 16 , 147 ). These studies represent the first time a CHIM had been used in an active pandemic ( 32 ). COVID CHIM studies provided unique data on the underlying pathophysiology ( 33 ); transmission of the disease and the accuracy of lateral-flow testing ( 32 ). However, recruitment to these studies still took over a year from the initial onset of the pandemic ( 31 ), by which time vaccines had been developed and licensed using traditional (accelerated) routes. It is recognised that creating an attenuated virus may take at least a year of study during a pandemic ( 36 ), and therefore we have searched for strains that could be used in advance of an epidemic or pandemic. Our scoping review has several strengths. To our knowledge, it is the first review to systematically investigate attenuated viral strains of priority pathogens that could be re-purposed into a CHIM. We involved an information specialist to develop our search strategy and adhered closely to both our pre-specified protocol and recognised methodology for a scoping review ( 29 ). In common with all scoping reviews, our findings are dependent on the quality of the included studies. Whilst all individual candidate strains had at least one study reporting an attenuated viraemia, only four of the nine included studies reported these rates precisely ( 40 , 41 , 44 , 45 , 47 ). There was also incomplete reporting of overall adverse event rates, with five studies not reporting these data ( 40 , 44 , 45 , 48 ). We made a pragmatic decision to focus the scoping review on six viruses on the CEPI priority pathogen list ( 5 ), rather than the 23 viruses on the WHO “list of emerging pathogens for a potential future pandemic” ( 1 ), due to logistical capacity. However, the same methodology and data collection framework could be applied to other viruses with pandemic potential in future work. In conclusion, there are three attenuated viral strains of two CEPI priority pathogens, CHIKV and RVFV, that have been administered to humans that cause a viraemia and may therefore be amenable to development into a novel CHIM. Of these, VLA1553 of CHIKV is in the most advanced stage of study. There is a research gap for the creation of new attenuated mutants that could be utilised in CHIM for other priority pathogens, but the availability of reverse genetics systems and sound knowledge of proteins such interferon antagonists, or codon deoptimization strategies could allow the future development of such attenuated viruses. Data Availability N/A = scoping review Funding Study supported by Research, Evidence and Development Initiative (READ-It), LSTM, which is funded by UK aid from the UK government for the benefit of low- and middle-income countries (project number 300342-104). The views expressed do not necessarily reflect the UK government’s official policies. Conflicts of interest The authors have no conflicts of interest to disclose References 1. ↵ Ukoaka BM , Okesanya OJ , Daniel FM , Ahmed MM , Udam NG , Wagwula PM , et al. Updated WHO list of emerging pathogens for a potential future pandemic: Implications for public health and global preparedness . 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