An Optimized Method for Nucleus Extraction from Saccharomyces cerevisiae

An Optimized Method for Nucleus Extraction from Saccharomyces cerevisiae | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article An Optimized Method for Nucleus Extraction from Saccharomyces cerevisiae liao taohsueh This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5191303/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract In eukaryotic cells,the nucleus is a vital organelle housing genetic material, including chromosomes and histones.Different tissues comprise various cell types,some possessing robust cell walls,such as Saccharomyces cerevisiae.While mammalian cells like HeLa are commonly used for nuclear extraction studies,yeast offers advantages in cultivation speed and simplicity.Building upon methodologies described by Gregory et al. ( 1998 ) and Cote et al. (1995),we have developed an improved protocol for extracting nuclei from yeast cells.This study investigates the efficiency of a cell nuclei extraction protocol using image-based quantification methods.Microscopic images were analyzed before and after the extraction process using ImageJ-equivalent computer vision techniques. The results show a significant reduction in nuclei count post-extraction,demonstrating the effectiveness of the applied method.This method provides a valuable tool for biologists requiring efficient nucleus extraction from yeast. Biological sciences/Biological techniques/Biological models Biological sciences/Biotechnology/Biologics Eukaryotic cells Nucleus extraction Saccharomyces cerevisiae Yeast Cell wall HeLa cells Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5191303","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":364787693,"identity":"f88d7b93-821b-4f38-a96f-716f21768de4","order_by":0,"name":"liao 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